Welcome guest, Login |
Thanks for your further question/comment. It has been sent to the author(s) of this protocol. You will receive a notification once your question/comment is addressed again by the author(s).
Meanwhile, it would be great if you could help us to spread the word about Bio-protocol.
RNA interference is a powerful genetic approach for efficiently silencing target genes. Expression of short hairpin RNAs (shRNAs) allows analysis of the consequences of stably silencing genes. This protocol describes a method to stably integrate shRNA constructs with pSUPER.retro vectors and Lipofectamine in RKO and H1299 cells. This method can be applied to cells from other lines with modification of drug selection and cell conditions. pSUPER.retro vectors can be also transfected into packaging cells by this same method to produce retroviral supernatants.
Materials and Reagents
This protocol was adapted from Cecchinelli et al. (2006). This work was supported by grants from Associazione Italiana per la Ricerca sul Cancro (to S. Soddu). I am particularly grateful to Dr. Cinzia Rinaldo and Dr. Andrea Prodosmo for technical support.
Bio-protocol's major goal is to make reproducing an experiment an easier task. If you have used this protocol, it would be great if you could share your experience by leaving some comments, uploading images or even sharing some videos. Please login to post your feedback.
Login | Register
About Us |
What's New |
Author Map |
Terms of Service |
Copyright & IP Policy |
© 2015 Bio-protocol LLC. ISSN: 2331-8325