Published: Vol 3, Iss 4, Feb 20, 2013 DOI: 10.21769/BioProtoc.331 Views: 9796
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Abstract
We have established the use of Candida albicans as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular networks including cellular metabolism and the cell cycle. Unlike the model lab yeast Saccharomyces cerevisiae, C. albicans is not subject to the Crabtree effect and hence grows aerobically in glucose when oxygen is present. Therefore the control of mitochondrial biogenesis in C. albicans is more typical of eukaryotic cells. C. albicans has a fully sequenced genome and there are many published tools for genetic manipulation facilitating Systems Biology approaches. The isolation of mitochondria (as described in the protocol: Preparation of Mitochondria from Candida albicans) (Hewitt et al., 2013) produces a more simplified system that can be interrogated using the standard tools of molecular biology. In addition, the import of radiolabelled proteins described in this protocol is a sensitive technique that can be used to determine details of kinetics and interactions of imported proteins.
Keywords: MitochondriaMaterials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The protocol was adapted from: Hewitt et al. (2012). The work in the Traven lab and Lithgow lab on Candida albicans mitochondria is supported by a project grant from the Australian National Health and Medical Research Council (APP1023973). V.H. is the recipient of an Australian Postgraduate Award.
References
Article Information
Copyright
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Hewitt, V. L., Lithgow, T. and Traven, A. (2013). Candida albicans Mitochondrial Protein Import Assay. Bio-protocol 3(4): e331. DOI: 10.21769/BioProtoc.331.
Category
Cell Biology > Cell-based analysis > Transport
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