Published: Vol 3, Iss 3, Feb 5, 2013 DOI: 10.21769/BioProtoc.323 Views: 96793
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Abstract
Peripheral blood mononuclear cells (PBMCs) are chiefly lymphocytes and monocytes. PBMCs are separated from the whole blood by a density gradient centrifugation method using Ficoll Histopaque.
Keywords: PBMCMaterials and Reagents
Equipment
Procedure
Recipes
Technical notes
Acknowledgments
The laboratory protocol was evolved over time in the senior authors’ laboratory using a template that was published in 1986 in Handbook of Experimental Immunology / edited by D. M. Weir; co-editors, L. A. Herzenberg, Caroline Blackwell, Leonore A. Herzenberg. Blackwell Scientific Publications. Institute of Life Sciences is funded by Department of Biotechnology, Govt of India and SP was supported by a fellowship grant from Indian Council of Medical Research.
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© 2013 The Authors; exclusive licensee Bio-protocol LLC.
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Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
Category
Immunology > Immune cell isolation > Lymphocyte
Cell Biology > Cell isolation and culture > Cell isolation
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