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In vitro Assay for Dendritic Spine Retraction of Hippocampal Neurons with Sparse Labeling

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Original research article

A brief version of this protocol appeared in:
The Journal of Neuroscience
Oct 2015

Abstract

Dendritic spines are the post-synaptic structures that play a central role in excitatory synaptic transmission. Developmental spinogenesis relies on a variety of stimuli such as those derived from cell-cell communication and their downstream signaling. Here, we describe an in vitro assay of dendritic spine retraction using hippocampal slice culture, in which individual neurons are sparsely and brightly labeled by the Supernova method, for the study of molecular mechanisms of spine development.

Keywords: Spine, Retraction assay, EphA, Spinogenesis

How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Iwata, R. and Iwasato, T. (2016). In vitro Assay for Dendritic Spine Retraction of Hippocampal Neurons with Sparse Labeling. Bio-protocol 6(18): e1937. DOI: 10.21769/BioProtoc.1937.
  2. Iwata, R., Matsukawa, H., Yasuda, K., Mizuno, H., Itohara, S. and Iwasato, T. (2015). Developmental RacGAP α2-chimaerin signaling is a determinant of the morphological features of dendritic spines in adulthood. J Neurosci 35(40): 13728-13744.
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