Published: Vol 6, Iss 2, Jan 20, 2016 DOI: 10.21769/BioProtoc.1707 Views: 10716
Reviewed by: Arsalan DaudiTie LiuAnonymous reviewer(s)
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Abstract
Soybean cyst nematode (SCN; Heterodera glycines), an obligate parasite of plants, is the most damaging pathogen of soybean, causing $469 to $818 million in soybean yield losses annually in the United States. However, there are no soybean cultivars available that are resistant to all SCN populations. Therefore, much research is being conducted to develop soybean cultivars resistant to SCN (Matthews et al., 2013; Matthews et al., 2014; Youssef et al., 2013). Here we describe the rearing and harvesting of SCN, as well as how SCN can be assayed by determining the Female Index.
Materials and Reagents
Equipment
Procedure
Note: All procedures must be in compliance with APHIS requirements to prevent nematodes from escaping the laboratory and greenhouse.
Recipes
Acknowledgments
This protocol is adapted from our previous work, including, Matthews et al. (2013) and Youssef et al. (2013). We thank Peggy MacDonald for technical support. Mention of trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U. S. Department of Agriculture or imply its approval to the exclusion of other products or vendors that also may be suitable. The authors have no conflict of interest.
References
Article Information
Copyright
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Matthews, B. F. and Youssef, R. M. (2016). Soybean Cyst Nematode, Heterodera glycines, Infection Assay Using Soybean Roots. Bio-protocol 6(2): e1707. DOI: 10.21769/BioProtoc.1707.
Category
Plant Science > Plant immunity > Disease bioassay
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