Ginseng refers to species within the genus Panax and is a slow-growing perennial herb from the Araliaceae family. The most widely used Panax species is Panax ginseng Meyer (Korean ginseng). Panax japonicus (Japanese ginseng), Panax notoginseng (Chinese ginseng), and Panax quinquefolium (American ginseng). Due to the various pharmaceutical importance of ginsenosides, ginseng plant has been cultivated for its highly valued root over 2,000 years as a medicinal plant in East Asian countries particularly in China, Korea, and Japan and North America. Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Cultivar “Yunpoong” has characteristics to have more axillary shoots and lateral roots compared to other cultivars. Thus “Yunpoong”: Ginseng seeds are relatively more feasible for regeneration of adventitious roots during gene transformation. Here, we describe how to prepare and treat ginseng seeds after harvest till the ginseng immature embryos are ready for the germination, and to be used in Agrobacterium tumefaciens-mediated gene transformation.
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