Published: Vol 4, Iss 23, Dec 5, 2014 DOI: 10.21769/BioProtoc.1308 Views: 47799
Reviewed by: Arsalan DaudiKanika GeraAnonymous reviewer(s)
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Abstract
Minimal inhibition concentration (MIC) is the lowest concentration of an antimicrobial agent that can inhibit the visible growth of a microorganism after overnight incubation. MIC determination is used as not only a diagnostic tool in treating bacterial infections for clinicians but also a research method in evaluating the efficacy of an antimicrobial. Multidrug resistance Acinetobacter baumannii (A. baumannii) has emerged in recent years. Accurate determination of resistance by MIC assay is important in coping with this superbug. Here we described a protocol for determining MIC for A. baumannii in hope of assisting researchers and physicians in confirming resistance of clinical isolates correctly.
Keywords: Acinetobacter baumanniiMaterials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The development of this protocol was funded by a grant from the National Taiwan University Hospital, Chu-Tung Branch.
References
Article Information
Copyright
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Lin, M., Lin, Y. and Lan, C. (2014). Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii. Bio-protocol 4(23): e1308. DOI: 10.21769/BioProtoc.1308.
Category
Microbiology > Antimicrobial assay > Antibacterial assay
Cell Biology > Cell isolation and culture > Cell growth
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