Published: Vol 4, Iss 6, Mar 20, 2014 DOI: 10.21769/BioProtoc.1084 Views: 13520
Reviewed by: Anonymous reviewer(s)
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Abstract
Two critical steps when investigating an animal model of a virus infection are consistently successfully infecting animals and accurately determining viral titers in tissue throughout the course of infection. Here we discuss in detail how to infect mice with SARS-CoV and then quantify the titer of virus in the lung.
Keywords: SARS-CoVMaterials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This work was supported by grants from the National Institutes of Health (PO1AI060699, RO1AI091322). The protocol described herein was based on the following manuscript: Zhao et al. (2010).
References
Article Information
Copyright
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Fett, C., Zhao, J. and Perlman, S. (2014). Virus Infection and Titration of SARS-CoV in Mouse Lung. Bio-protocol 4(6): e1084. DOI: 10.21769/BioProtoc.1084.
Category
Microbiology > Microbe-host interactions > In vivo model
Microbiology > Microbe-host interactions > Virus
Cell Biology > Tissue analysis > Tissue isolation
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