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Limiting Dilution Assays to Determine Frequencies of Lymphohematopoietic Progenitors   

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Original research article

A brief version of this protocol appeared in:
Immunity
Jun 2013

Abstract

This protocol is useful to determine the frequencies of lymphohematopoietic progenitors in tested samples. To effectively support the growth and differentiation of primitive lymphohematopoietic progenitors, complex signals from stromal cells are important. Several stromal cell lines are known to support both lymphoid and myeloid cells simultaneously in mouse. In this protocol, we introduce two stromal co-culture systems for murine lymphohematopoietic progenitors and their application for limiting dilution assays.

Copyright: © 2014 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Yokota, T. and Satoh, Y. (2014). Limiting Dilution Assays to Determine Frequencies of Lymphohematopoietic Progenitors. Bio-protocol 4(4): e1045. DOI: 10.21769/BioProtoc.1045.
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