搜索

Plant Materials and Growth Conditions of Japanese Morning Glory (Ipomoea nil cv. Violet)
日本牵牛花培养条件的建立及植物材料的分析   

评审
匿名评审
下载 PDF 引用 收藏 提问与回复 分享您的反馈 Cited by

本文章节

Abstract

Japanese morning glory (Ipomoea nil) is a summer annual vine that typically produces ephemeral flowers. This plant has been used extensively to investigate flowers, including studies on flowering, flower color, and petal senescence. Here we describe the materials and optimal growth conditions used to grow the Japanese morning glory cultivar, “Violet”, in a growth chamber. Violet plants are transformable with Agrobacterium (Rhizobium), and we have used this cultivar to examine petal senescence (Shibuya et al., 2009; Shibuya et al., 2014).

Keywords: Morning glory(牵牛花), Ipomoea(甘薯), Flower(花), Plant(植物)

Materials and Reagents

  1. Japanese morning glory cultivar, “Violet” (Ipomoea nil cv. Violet) seeds
    Note: Seeds of the Japanese morning glory cultivar, “Violet” (Ipomoea nil cv. Violet), as well as other cultivars can be obtained through the National BioResource Project, Japan (http://www.shigen.nig.ac.jp/asagao/).
  2. Horticultural soil (Nippi Horticulture soil No. 1; Nihon Hiryo)
  3. Fertilizer (Hyponex, catalog number: 15-30-15 )

Equipment

  1. Growth chamber
  2. 12 cm-diameter pots
  3. Metal wire (1 m)
  4. Utility knife

Procedure

  1. Soil preparation
    1. We use a granular horticultural soil with fertilizer (Nippi Horticulture soil No. 1) but most types of horticultural soil for flowers or vegetables can be used to grow Japanese morning glory.
    2. Place the soil into pots; we use 12 cm-diameter pots with a hole in the bottom. The hole is covered with a net to prevent the loss of soil, and the soil is moistened.

  2. Seed preparation
    Shave off a thin slice of the seed coat opposite the embryo with a knife to increase imbibition (Figure 1), taking care not to make the incision too deep. If you see the white underlying endosperm, then the depth of the incision is sufficient. This procedure ensures that the time required to germination is more uniform.


    Figure 1. A Violet seed with a thin slice of the seed coat removed. E, Embryo; *area of shaved seed coat

  3. Planting
    1. Plant the prepared seeds in soil at a depth of 1-1.5 cm (one seed per pot).
    2. To grow the Violet plants, place the pots on a tray.
    3. The tray containing the pots is then placed in a growth chamber at 24 °C and a relative humidity of 70%. The photoperiod used is 16L/8D (light/dark) with light provided by white-fluorescent lamps at approximately 100 µmol/m/s. Plants typically germinate after 3-4 days.
    4. Plants are grown for 1 month under the 16L/8D long-day photoperiod.
    5. Plants are fertilized with 1 g/L Hyponex 15-30-15 (150-200 ml per pot) once a week after the first true leaf expands.
      Notes:
      1. There are unlikely to be any pests in a clean growth chamber. However, if whiteflies are problem, scatter granular “Orthoran” on the soil surface after germination.
      2. We pinch the tip of vine to induce axillary bud formation when the plants have five true leaves (Figure 2), but this procedure is not essential for flower production.


      Figure 2. A drawing of the Violet plant with five true leaves. An arrow indicates a position for pinching out the vine.

    6. Wind the developing vines around a support. We use metal wire as a support (Figure 3).
    7. After 1 month, change the photoperiod to a 12L/12D cycle to induce flowering.
      Note: The Japanese morning glory is an absolute short-day plant. If you want to produce flowers more quickly, place the pots under a photoperiod of 12L/12D from the very beginning (i.e. immediately after sowing). If you want to induce the production of larger plants with more flowers, grow the plants under a long-day photoperiod (16L/8D) for more than 1 month.
    8. The plants should produce flower buds approximately 1 month after the photoperiod is changed to the 12L/12D cycle (Figure 3).
      Notes:
      1. If the flowers are removed before the seeds are set (preferably, before the flowers wilt) then the plants will keep flowering for several months as seed setting markedly increases plant death.
      2. Under a photoperiod of 12L/12L, the flowers will be almost fully open at the start of the light period.
      3. If you want to harvest seeds, mature seeds can be obtained approximately 6 weeks after the flowers open.


      Figure 3. A Violet plant after 11 weeks in a growth chamber

Acknowledgments

This work was supported by grants from the National Agriculture and Food Research Organization, Japan. We thank the National BioResource Project, Japan for providing seeds of Japanese morning glory cultivar, “Violet”.

References

  1. Shibuya, K., Shimizu, K., Niki, T. and Ichimura, K. (2014). Identification of a NAC transcription factor, EPHEMERAL1, that controls petal senescence in Japanese morning glory. Plant J 79(6): 1044-1051.
  2. Shibuya, K., Yamada, T., Suzuki, T., Shimizu, K. and Ichimura, K. (2009). InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Japanese morning glory. Plant Physiol 149(2): 816-824.

简介

日本牵牛花( Ipomoea nil )是一种夏天的年生藤,通常产生短暂的花。 这种植物已被广泛用于调查花,包括开花,花色和花瓣衰老的研究。 在这里,我们描述了用于在生长室中培养日本牵牛花栽培品种"紫罗兰"的材料和最佳生长条件。 紫色植物可用土壤杆菌属(根瘤菌)进行转化,并且我们使用该栽培种来检查花瓣衰老(Shibuya等人,2009; Shibuya et al。,2014)。

关键字:牵牛花, 甘薯, 花, 植物

材料和试剂

  1. 日本牵牛花品种"紫罗兰"(番薯属 cv。紫罗兰)种子
    注意:日本牵牛花品种"紫罗兰"(番薯)的种子以及其他品种可以通过日本国家生物资源计划获得(http://www.shigen.nig.ac.jp/asagao/)。
  2. 园艺土壤(Nippi Horticulture soil No.1; Nihon Hiryo)
  3. 肥料(Hyponex,目录号:15-30-15)

设备

  1. 生长室
  2. 12厘米直径的锅
  3. 金属线(1 m)
  4. 实用刀

程序

  1. 土壤准备
    1. 我们使用粒状园艺土壤与肥料(尼皮园艺   土壤1号),但大多数类型的园艺土壤为花或 蔬菜可以用来种植日本牵牛花。
    2. 放置   土壤 我们使用12厘米直径的锅底部有一个洞。 孔用网覆盖以防止土壤和土壤的损失   被润湿。

  2. 种子准备
    用刀子刮掉胚胎对面的种皮的薄片以增加吸收(图1),注意不要使切口太深。 如果你看到白色的底层胚乳,那么切口的深度是 足够。 该程序确保发芽所需的时间更均匀。


    图1.除去种皮薄片的紫罗兰种子。 E,胚胎; *刨花面积

  3. 种植
    1. 将准备的种子播种在土壤中,深度为1-1.5厘米(每盆一粒种子)
    2. 要种植紫罗兰植物,将盆放在托盘上。
    3. 然后将含有罐的托盘放置在生长室中 24℃和相对湿度为70%。 使用的光周期为16L/8D (亮/暗)与由白色荧光灯提供的光 约100μmol/m/s。 植物通常在3-4天后发芽
    4. 植物在16L/8D长日光周期下生长1个月。
    5. 在第一次真叶膨胀后,植物每周用1g/L Hyponex 15-30-15(每盆150-200ml)受精。
      注意:
      1. 在清洁的生长室中不可能有任何害虫。 然而,如果粉虱是问题,散射颗粒"Orthoran"上 土壤表面发芽后。
      2. 我们捏藤蔓的尖端 当植物有五个真叶时诱导腋芽形成 (图2),但此程序对花卉生产不是必需的。


      图2.紫罗兰植物有五片真叶的图。 箭头表示拉出葡萄树的位置。

    6. 将发展中的葡萄树绕在支撑物上。 我们使用金属丝作为支撑(图3)。
    7. 1个月后,将光周期更改为12L/12D周期以诱导开花 注意:日本牵牛花是一个绝对的短日植物。 如果你   想要更快地生产花,把盆放在下 12L/12D的光周期从一开始(即紧接着) 播种)。 如果你想诱导生产更大的植物   花,在长日光周期(16L/8D)下种植植物更多   超过1个月。
    8. 植物应该产生花芽 大约在光周期变为12L/12D后1个月 循环(图3)。
      注意:
      1. 如果花以前被删除 种子被设置(优选地,在花枯萎之前)然后植物 将保持开花几个月作为种子设置显着 增加植物死亡。
      2. 在12L/12L的照片周期下,花将几乎完全打开。
      3. 如果你想收获种子,成熟的种子可以在大约开花6周后获得。


      图3.在生长室中11周后的紫罗兰植物

致谢

这项工作得到了日本国家农业和食品研究组织的资助。 我们感谢国家生物资源项目,日本提供日本牵牛花栽培品种"紫罗兰"的种子。

参考文献

  1. Shibuya,K.,Shimizu,K.,Niki,T。和Ichimura,K。(2014)。 鉴定NAC转录因子,EPHEMERAL1,其控制日本牵牛花中的花瓣衰老。/a> Plant J 79(6):1044-1051
  2. Shibuya,K.,Yamada,T.,Suzuki,T.,Shimizu,K.and Ichimura,K。(2009)。 InPSR26,一种假定的膜蛋白,在日本牵牛花的花瓣衰老期间调节程序性细胞死亡。 a> Plant Physiol 149(2):816-824。
  • English
  • 中文翻译
免责声明 × 为了向广大用户提供经翻译的内容,www.bio-protocol.org 采用人工翻译与计算机翻译结合的技术翻译了本文章。基于计算机的翻译质量再高,也不及 100% 的人工翻译的质量。为此,我们始终建议用户参考原始英文版本。 Bio-protocol., LLC对翻译版本的准确性不承担任何责任。
Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.
引用:Shibuya, K. (2015). Plant Materials and Growth Conditions of Japanese Morning Glory (Ipomoea nil cv. Violet). Bio-protocol 5(10): e1478. DOI: 10.21769/BioProtoc.1478.
提问与回复

(提问前,请先登录)bio-protocol作为媒介平台,会将您的问题转发给作者,并将作者的回复发送至您的邮箱(在bio-protocol注册时所用的邮箱)。为了作者与用户间沟通流畅(作者能准确理解您所遇到的问题并给与正确的建议),我们鼓励用户用图片或者视频的形式来说明遇到的问题。由于本平台用Youtube储存、播放视频,作者需要google 账户来上传视频。

当遇到任务问题时,强烈推荐您提交相关数据(如截屏或视频)。由于Bio-protocol使用Youtube存储、播放视频,如需上传视频,您可能需要一个谷歌账号。