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Cancer Biology

Measurement of Cytokines Authors:  Renaud Grépin and Gilles Pagès, date: 11/20/2012, view: 6346, Q&A: 2
This protocol allows to measure the levels cytokines - such as VEGFs, CXCLs cytokines, PDGF or FGF - from fresh samples but also frozen tumors. The advantage of this method is to use very few micrograms of biological material and the protocol is carried out quickly.
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Measurement of Hemoglobin Authors:  Renaud Grépin and Gilles Pagès, date: 11/20/2012, view: 10285, Q&A: 1
This protocol allows to measure the levels of intratumoral hemoglobin from human or rodent fresh samples but also frozen tumors. The advantage of this method is to use very few microliters of biological material for hemoglobin and the protocol is carried out quickly.
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Cell Biology

Measurement of Free Cytosolic Calcium Concentration ([Ca2+]i) in Single CHO-K1 Cells Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 6273, Q&A: 0
This is a protocol to analyze the functional response of single CHO-K1 cells to a given treatment in terms of changes in free cytosolic calcium concentration ([Ca2+]i). This is possible by using the Ca2+ indicator dye Fura-2 AM, a polyamino carboxylic acid that binds to free intracellular calcium and is excited at 340 nm and 380 nm. The ratio of ...
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Immunology

Determination of Toxoplasma gondii Replication in Naïve and Activated Macrophages Authors:  Emma Iaconetti, Brian Lynch, Nathaniel Kim and Dana G. Mordue, date: 11/20/2012, view: 6214, Q&A: 0
Toxoplasma gondii is an obligate intracellular protozoan parasite that causes the disease toxoplasmosis. Chronic infection is established through the formation of tissue cysts predominantly in cardiac and neurologic tissues. A defining characteristic of T. gondii is its ability to evade the host’s immune defenses; specifically, ...
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Molecular Biology

Isolation of Epithelial Cells from Mouse Gastrointestinal Tract for Western Blot or RNA Analysis Authors:  Maged Zeineldin and Kristi Neufeld, date: 11/20/2012, view: 10626, Q&A: 0
The gastrointestinal (GI) tract is lined by a single layer of epithelial cells which function in secretion, absorption, and digestion. In addition, most GI tract tumors develop from epithelial cells (carcinomas). This protocol describes isolation of the surface epithelium from the underlying stroma, muscular layer and submucosa in the GI tract. In ...
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GPCRs Interaction Measurement by Fluorescence Resonance Energy Transfer (FRET) Authors:  Manuel D. Gahete, Raúl M. Luque and Justo P. Castaño, date: 11/20/2012, view: 4631, Q&A: 0
This is a protocol to determine the physical interaction of a G-protein coupled receptor (GPCR) with itself (homodimerization) or with other GPCR (heterodimerazation) using fluorescence resonance energy transfer (FRET). FRET is a distance-dependent interaction between the electronic excited states of two dye molecules (in this case, CFP and YFP) ...
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Plant Science

Extraction of Coumarins from Leaves, Petioles, Stems and Roots of Ruta graveolens and Nicotiana benthamiana Authors:  Alain Hehn, Guilhem Vialart, Alexandre Olry and Frederic Bourgaud, date: 11/20/2012, view: 5249, Q&A: 0
This method describes the extraction of coumarins and furanocoumarins from leaves of Ruta graveolens (a natural furanocoumarin) producer, and Nicotiana benthamiana.
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Stem Cell

Mouse ESC Differentiation to Nkx2.1+ Lung and Thyroid Progenitors Authors:  Tyler A. Longmire, Laertis Ikonomou and Darrell N. Kotton, date: 11/20/2012, view: 6216, Q&A: 0
The de novo derivation of lung progenitors from pluripotent stem cells provides the opportunity to model early lung development in vitro and allows easy access to cells for tissue engineering or basic cell biology studies. This detailed protocol allows the generation of lung and thyroid progenitors from mouse embryonic stem cell ...
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