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Zhengjian Zhang


Ph.D., Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, 2004

Current position

Senior Scientist, Transcription Imaging Consortium, Janelia Research Campus of HHMI (2013-present)


  1. Zhang, Z.*, Boskovic, Z., Hussain, M. M., Hu, W., Inouye, C., Kim, H. J., Abole, A. K., Doud, M. K., Lewis, T. A., Koehler, A. N., Schreiber, S. L. and Tjian, R. (2015). Chemical perturbation of an intrinsically disordered region of TFIID distinguishes two modes of transcription initiation. Elife 4. (* Corresponding author)
  2. Grimm, J. B., English, B. P., Chen, J., Slaughter, J. P., Zhang, Z., Revyakin, A., Patel, R., Macklin, J. J., Normanno, D., Singer, R. H., Lionnet, T. and Lavis, L. D. (2015). A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods 12(3): 244-250, 243 p following 250.
  3. Chen, J., Zhang, Z., Li, L., Chen, B. C., Revyakin, A., Hajj, B., Legant, W., Dahan, M., Lionnet, T., Betzig, E., Tjian, R. and Liu, Z. (2014). Single-molecule dynamics of enhanceosome assembly in embryonic stem cells. Cell 156(6): 1274-1285.
  4. Zhang, Z.*, Revyakin, A.*, Grimm, J. B., Lavis, L. D. and Tjian, R. (2014). Single-molecule tracking of the transcription cycle by sub-second RNA detection. Elife 3: e01775. (* Co-first and corresponding authors)
  5. Zhou, H., Kaplan, T., Li, Y., Grubisic, I., Zhang, Z., Wang, P. J., Eisen, M. B. and Tjian, R. (2013). Dual functions of TAF7L in adipocyte differentiation. Elife 2: e00170.
  6. Revyakin, A.*, Zhang, Z.*, Coleman, R. A., Li, Y., Inouye, C., Lucas, J. K., Park, S. R., Chu, S. and Tjian, R. (2012). Transcription initiation by human RNA polymerase II visualized at single-molecule resolution. Genes Dev 26(15): 1691-1702. (* Co-first authors)
  7. Tomar, R. S., Psathas, J. N., Zhang, H., Zhang, Z. and Reese, J. C. (2009). A novel mechanism of antagonism between ATP-dependent chromatin remodeling complexes regulates RNR3 expression. Mol Cell Biol 29(12): 3255-3265.
  8. Reese, J. C., Zhang, H. and Zhang, Z. (2008). Isolation of highly purified yeast nuclei for nuclease mapping of chromatin structure. Methods Mol Biol 463: 43-53.
  9. Zhang, Z. and Reese, J. C. (2006). Isolation of yeast nuclei and micrococcal nuclease mapping of nucleosome positioning. Methods Mol Biol 313: 245-255.
  10. Zhang, Z. and Reese, J. C. (2005). Molecular genetic analysis of the yeast repressor Rfx1/Crt1 reveals a novel two-step regulatory mechanism. Mol Cell Biol 25(17): 7399-7411.
  11. Zhang, Z. and Reese, J. C. (2004). Redundant mechanisms are used by Ssn6-Tup1 in repressing chromosomal gene transcription in Saccharomyces cerevisiae. J Biol Chem 279(38): 39240-39250.
  12. Zhang, Z. and Reese, J. C. (2004). Ssn6-Tup1 requires the ISW2 complex to position nucleosomes in Saccharomyces cerevisiae. EMBO J 23(11): 2246-2257.
  13. Reese, J. C., Zhang, Z. and Kurpad, H. (2000). Identification of a yeast transcription factor IID subunit, TSG2/TAF48. J Biol Chem 275(23): 17391-17398.

Protocols by Zhengjian Zhang
  1. Cloud-point PEG Glass Surfaces for Imaging of Immobilized Single Molecules by Total-internal-reflection Microscopy