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Jeffrey H. Withey


Ph.D. in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI, 2000

Current position

Associate Professor, Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI (2013-present)


*Equal contributors
#Denotes a WSU Ph.D. student in my laboratory

  1. Withey, J. H., Nag, D., Plecha, S. C.#, Sinha, R. and Koley, H. (2015). Conjugated linoleic acid reduces cholera toxin production in vitro and in vivo by inhibiting Vibrio cholerae ToxT activity. Antimicrob Agents Chemother 59(12): 7471-7476.
  2. Plecha, S. C. and Withey, J. H. (2015). [14C] Linoleic acid uptake and fractionation assay in Vibrio cholerae. Bio-protocol 5(24): e1682.
  3. Nag, D., Koley, H., Sinha, R., Mukherjee, P., Sarkar, C., Withey, J. H. and Gachhui, R. (2015). Immunization of mice with a live transconjugant Shigella hybrid strain induced Th1 and Th17 cell mediated immune responses and confirmed passive protection against heterologous Shigellae. Scand J Immunol. (Epub ahead of print)
  4. Plecha, S. C.# and Withey, J. H. (2015). Mechanism for inhibition of Vibrio cholerae ToxT activity by the unsaturated fatty acid components of bile. J Bacteriol 197(10): 1716-1725.
  5. Thomson, J. J.#, Plecha, S. C. and Withey, J. H. (2015). A small unstructured region in Vibrio cholerae ToxT mediates the response to positive and negative effectors and ToxT proteolysis. J Bacteriol 197(3): 654-668.
  6. Park, B., Zielke, R., Wierzbicki, I., Mitchell, K. C.#, Withey, J. H., and Sikora, A. (2015). A new metalloprotease secreted by the Type II Secretion System links Vibrio cholerae with collagen. J Bacteriol 197: 1051-1064.
    This publication also produced the cover photo for this issue of Journal of Bacteriology.
  7. Rowe, H. M., Withey, J. H. and Neely, M. N. (2014). Zebrafish as a model for zoonotic aquatic pathogens. Dev Comp Immunol 46(1): 96-107.
  8. Thomson, J. J.# and Withey, J. H. (2014). Bicarbonate increases binding affinity of Vibrio cholerae ToxT to virulence gene promoters. J Bacteriol 196(22): 3872-3880.
  9. Runft, D. L., Mitchell, K. C.#, Abuaita, B. H.#, Allen, J. P., Bajer, S., Ginsburg, K., Neely, M. N. and Withey, J. H. (2014). Zebrafish as a natural host model for Vibrio cholerae colonization and transmission. Appl Environ Microbiol 80(5): 1710-1717.
  10. Dittmer, J. B.# and Withey, J. H. (2012). Identification and characterization of the functional toxboxes in the Vibrio cholerae cholera toxin promoter. J Bacteriol 194(19): 5255-5263.
  11. Abuaita, B. H.# and Withey, J. H. (2011). Termination of Vibrio cholerae virulence gene expression is mediated by proteolysis of the major virulence activator, ToxT. Mol Microbiol 81(6): 1640-1653.
  12. Abuaita, B. H.# and Withey, J. H. (2011). Genetic screening for bacterial mutants in liquid growth media by fluorescence-activated cell sorting. J Microbiol Methods 84(1): 109-113.
  13. Richard, A. L.*, Withey, J. H.*, Beyhan, S., Yildiz, F. and DiRita, V. J. (2010). The Vibrio cholerae virulence regulatory cascade controls glucose uptake through activation of TarA, a small regulatory RNA. Mol Microbiol 78(5): 1171-1181.
  14. Abuaita, B. H.# and Withey, J. H. (2009). Bicarbonate Induces Vibrio cholerae virulence gene expression by enhancing ToxT activity. Infect Immun 77(9): 4111-4120.
  15. Withey, J. H. (2009). Control of Vibrio cholerae virulence gene expression by ToxT. Research Advances in Bacteriology.
  16. Bellair, M. and Withey, J. H. (2008). Flexibility of Vibrio cholerae ToxT in transcription activation of genes having altered promoter spacing. J Bacteriol 190(24): 7925-7931.
  17. Matson, J. S., Withey, J. H. and DiRita, V. J. (2007). Regulatory networks controlling Vibrio cholerae virulence gene expression. Infect Immun 75(12): 5542-5549.
  18. Withey, J. H. and DiRita, V. J. (2006). The toxbox: specific DNA sequence requirements for activation of Vibrio cholerae virulence genes by ToxT. Mol Microbiol 59(6): 1779-1789.
  19. Withey, J. H. and DiRita, V. J. (2005). Activation of both acfA and acfD transcription by Vibrio cholerae ToxT requires binding to two centrally located DNA sites in an inverted repeat conformation. Mol Microbiol 56(4): 1062-1077.
  20. Withey, J. H. and Dirita, V. J. (2005). Vibrio cholerae ToxT independently activates the divergently transcribed aldA and tagA genes. J Bacteriol 187(23): 7890-7900.
  21. Withey, J. H. and Friedman, D. I. (2003). A salvage pathway for protein structures: tmRNA and trans-translation. Annu Rev Microbiol 57: 101-123.
  22. Withey, J. H. and Friedman, D. I. (2002). The biological roles of trans-translation. Curr Opin Microbiol 5(2): 154-159.
  23. Huang, C., Wolfgang, M. C., Withey, J., Koomey, M. and Friedman, D. I. (2000). Charged tmRNA but not tmRNA-mediated proteolysis is essential for Neisseria gonorrhoeae viability. EMBO J 19(5): 1098-1107.
  24. Withey, J. and Friedman, D. (1999). Analysis of the role of trans-translation in the requirement of tmRNA for lambdaimmP22 growth in Escherichia coli. J Bacteriol 181(7): 2148-2157.
Protocols by Jeffrey H. Withey
  1. [14C] Linoleic Acid Uptake and Fractionation Assay in Vibrio cholerae