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Coauthors
Patrick Sexton Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Australia
2 protocols

Denise Wootten Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Australia
2 protocols

Cassandra Koole
  • Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Australia
Contributions
  • 2 Author merit

Education

Ph.D., Department of Pharmacology, Monash University, Melbourne, Australia, 2012

Current position

NHMRC CJ Martin Postdoctoral Research Fellow

Publications

  1. Koole, C., Pabreja, K., Savage, E. E., Wootten, D., Furness, S. G., Miller, L. J., Christopoulos, A. and Sexton, P. M. (2013). Recent advances in understanding GLP-1R (glucagon-like peptide-1 receptor) function. Biochem Soc Trans 41(1): 172-179.
  2. Harikumar, K. G., Wootten, D., Pinon, D. I., Koole, C., Ball, A. M., Furness, S. G., Graham, B., Dong, M., Christopoulos, A., Miller, L. J. and Sexton, P. M. (2012). Glucagon-like peptide-1 receptor dimerization differentially regulates agonist signaling but does not affect small molecule allostery. Proc Natl Acad Sci U S A 109(45): 18607-18612.
  3. Koole, C., Wootten, D., Simms, J., Savage, E. E., Miller, L. J., Christopoulos, A. and Sexton, P. M. (2012). Second extracellular loop of human glucagon-like peptide-1 receptor (GLP-1R) differentially regulates orthosteric but not allosteric agonist binding and function. J Biol Chem 287(6): 3659-3673.
  4. Koole, C., Wootten, D., Simms, J., Miller, L. J., Christopoulos, A. and Sexton, P. M. (2012). Second extracellular loop of human glucagon-like peptide-1 receptor (GLP-1R) has a critical role in GLP-1 peptide binding and receptor activation. J Biol Chem 287(6): 3642-3658.
  5. Koole, C., Wootten, D., Simms, J., Valant, C., Miller, L. J., Christopoulos, A. and Sexton, P. M. (2011). Polymorphism and ligand dependent changes in human glucagon-like peptide-1 receptor (GLP-1R) function: allosteric rescue of loss of function mutation. Mol Pharmacol 80(3): 486-497.
  6. Wootten, D., Simms, J., Koole, C., Woodman, O. L., Summers, R. J., Christopoulos, A. and Sexton, P. M. (2011). Modulation of the glucagon-like peptide-1 receptor signaling by naturally occurring and synthetic flavonoids. J Pharmacol Exp Ther 336(2): 540-550.
  7. Koole, C., Wootten, D., Simms, J., Valant, C., Sridhar, R., Woodman, O. L., Miller, L. J., Summers, R. J., Christopoulos, A. and Sexton, P. M. (2010). Allosteric ligands of the glucagon-like peptide 1 receptor (GLP-1R) differentially modulate endogenous and exogenous peptide responses in a pathway-selective manner: implications for drug screening. Mol Pharmacol 78(3): 456-465.
  8. Koole, C., Wootten, D., Sexton, P.M. cAMP accumulation assays using the ALPHAscreen® kit (PerkinElmer). Bio-protocol.org, 3(13):http://www.bio-protocol.org/wenzhang.aspx?id=805.
  9. Koole, C., Wootten, D., Sexton, P.M. pERK detection assays using the Surefire ALPHAscreen® kit (TGR Biosciences and PerkinElmer). Bio-protocol.org, 3(13):http://www.bio-protocol.org/wenzhang.aspx?id=806.
2 Protocols published
pERK Detection Assays Using the Surefire AlphaScreen® Kit (TGR Biosciences and PerkinElmer)
Authors:  Cassandra Koole, Denise Wootten and Patrick Sexton, date: 07/05/2013, view: 4532, Q&A: 0
Extracellular signal-regulated kinase 1 and 2 (ERK1/2) are serine/threonine protein kinases that are phosphorylated on Thr202/Tyr204 (ERK1) and Thr185/Tyr187 (ERK2). Phosphorylation of ERK1/2 (pERK1/2) arises from multiple stimuli, resulting in ...
cAMP Accumulation Assays Using the AlphaScreen® Kit (PerkinElmer)
Authors:  Cassandra Koole, Denise Wootten and Patrick Sexton, date: 07/05/2013, view: 5918, Q&A: 0
Cyclic adenosine monophosphate (cAMP) is an intracellular signaling messenger derived from the catalytic conversion of ATP, and is a major product of activated Gs protein-coupled receptors. Conversely, formation of cAMP is inhibited by Gi ...