Abstract

Single-cell genomic technologies have revolutionized the way we study cell function and gene expression. However, these methods often require immediate sample processing to preserve fragile cell populations, posing logistical challenges for decentralized workflows and multi-center studies. FixNCut, a reversible sample fixation strategy followed by tissue dissociation, addresses these limitations by decoupling sample collection from downstream processing steps. By applying FixNCut, RNA and DNA integrity are preserved and cellular composition is maintained while minimizing stress-related artifacts. 

In this webinar, we present an optimized guide to the FixNCut protocol, showcasing its utility for single-cell genomic assays like scRNA-seq and ATAC-seq. We will highlight how FixNCut enables a robust, reproducible workflow suitable for various biological samples and unconventional study designs. Join us to explore how this innovative approach minimizes artifacts, preserves cellular integrity, and facilitates collaborative research across diverse settings.

Highlights

- Introduction to FixNCut protocol: A versatile method for decoupling sample collection from downstream processing steps.

- Optimization for single-cell assays: Supports scRNA-seq, scATAC-seq, and other single-cell techniques involving cell labeling and spatial technologies.

- Minimizing artifacts and enabling collaboration: Reduces artifacts and supports multi-center research in diverse settings.