Abstract

Structural DNA nanotechnology has enabled the creation of user-defined DNA origami nanostructures (DNs) for diverse applications such as drug delivery, vaccines, and biosensors. However, understanding DN interactions and distribution within cells and tissues has been limited by the drawbacks of current tracking methods, including low sensitivity and dye-induced artifacts.

OrigamiFISH addresses these challenges by targeting conserved DN scaffold sequences with hybridization chain reaction probes, achieving thousand-fold signal amplification. This innovative technique reveals cell type–specific and DN shape–specific distribution patterns within minutes of uptake at picomolar concentrations, far surpassing the sensitivity of existing methods. Additionally, origamiFISH is optimized for compatibility with immunofluorescence and tissue clearing, enabling detailed visualization of DN distribution in various biological contexts.

This webinar will cover the principles of origamiFISH, demonstrate its application in visualizing DNs across different microscopy modalities, and discuss its potential for advancing DN-based therapeutic development. 

Highlights

- Introduction to origamiFISH

- Technical innovations and biological applications of origamiFISH

- Compatibility and visualization techniques associated with origamiFISH

- Impact on DNA Nanotechnology