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MIC-Drop- a platform for large-scale in vivo CRISPR screens

Speaker: Saba Parvez Moderator: Zach Brandt

Online live: Sep 05, 2023 12:00 PM EST Posted: Oct 10, 2023 Views: 4294

Q&A Q&A

Abstract

CRISPR-Cas9 is a powerful tool to interrogate gene function in a targeted and systematic manner. Although the technology has been scaled up for large-scale genetic screens in cell culture, similar scale screens in vivo have been extremely challenging due to the cost, labor, and time required to generate and keep track of thousands of mutant animals. We reported the development of Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform that makes large-scale reverse genetic screens possible in zebrafish. In this webinar, we will share the details of this technique and discuss best practices for performing MIC-Drop screens in zebrafish.


The speakers will discuss:

·  Steps to successfully perform a large-scale CRISPR screen in zebrafish using the MIC-Drop platform

·  Possible directions to expand the applicability of MIC-Drop

Speaker

Saba Parvez

Saba Parvez, Ph.D.

Postdoctoral fellow, Department of Pharmacology & Toxicology, University of Utah, Salt Lake City, UT

Saba Parvez is a postdoctoral fellow in Dr. Randy Peterson’s laboratory at the University of Utah. Saba received his PhD in Chemistry and Chemical ...

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Moderator

Zach Brandt

Zach Brandt, Ph.D.

Postdoctoral fellow, Department of Pharmacology & Toxicology, University of Utah

Zach Brandt is a postdoctoral fellow in Dr. Randall Peterson’s laboratory at the University of Utah. Zach received his PhD in Cell and Developmenta...

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Keywords

Zebrafish, High-throughput CRISPR screen, Microfluidics

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31 Q&A

Fundamentals in CRISPR - Cas 9

How to design the sequence of the gene to be modified with crispr cas9? What are the steps from choosing the gene to preparing it for order?

I have observed others using snap gene, I am not confident in navigating through it.

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edit 2 Answers 13 Views Aug 24, 2023
SK SIYAB KHAN

NCBI or other platform are used to find functional region in gene(exon).and for that specific region you will design sgRNA.

helpful 0 helpful
NM Ngueliatou Njoyong Morain

Here, DNA sequencing helps you identify your target(s) genes. After which you design your guide dsRNA molecule.

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I will be more then happy if they can suggest how we can use this technique in plant science application
edit 1 Answer 3 Views Aug 23, 2023
NM Ngueliatou Njoyong Morain

Yes, it is possible. For example, in the production of genetically modified plants resistant to drought and specific diseases. Also by reducing the expression of the cellulose gene one could have plant biomass more suited for bio-ethanol production.

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que precauciones se podria tener en cuenta SOBRE platafomas MIC-Drop?

que precauciones se podria tener en cuenta SOBRE platafomas MIC-Drop?

edit 0 Answer 9 Views Aug 3, 2023

Has CRISPR been used successfully in cure of any human ailments?
edit 0 Answer 6 Views Aug 23, 2023

How canIusing CRISPR technology for build up biomedical device for disease detection
edit 0 Answer 6 Views Aug 23, 2023

CRISPR technology experiments biosafety and biosecurity in animal studies

Will CRISPR protocols be available for all to adopt?

edit 0 Answer 5 Views Aug 24, 2023

Can it be applied to other species? If so, what modifications are necessary? Example, C. elegance or plants such as Arabidopsis

If we are working on other species and want to implement this technology in our target species, what modifications can be done to the system? Example species would be C. elegance, plant species such as Arabidopsis

edit 0 Answer 5 Views Aug 24, 2023

Can be use this technology for anticancer therapies
edit 0 Answer 4 Views Nov 10, 2023

How the technology could be optimized to identify key functional genes in the gut microbiome contributing to dysbiosis and gut inflammation?
edit 0 Answer 3 Views Aug 25, 2023

How to identify the mutants generated by crispr using cost effective n fastest way?
edit 0 Answer 3 Views Aug 23, 2023

It's protocolfor utilisationim both invivo and invitr.

I want it to be used in conjunction to microfluidics and make something of a point care device for theranoatics.


edit 0 Answer 3 Views Aug 23, 2023

What will be the implementation of CRISPR technology in developing drugs against MDR/PDR/XDR organisms?
edit 0 Answer 3 Views Aug 23, 2023

What is MIC-Drop- a platform

What is MIC-Drop- a platform

edit 0 Answer 3 Views Aug 23, 2023

How does this method accounts for cell and tissue selectivity , precision and DNA damage responses?

Most diseases are due to a several mutations in different cells and tissue

edit 0 Answer 2 Views Sep 4, 2023

posibility to expand to C. elegans
edit 0 Answer 2 Views Aug 31, 2023

Which features of cell line(s) determines the number of guide RNA used to silence the target gene and what are the considerations for their design?
edit 0 Answer 2 Views Aug 25, 2023

How to resolve the current problem of having to do Multiple Knockouts in In-vivo models?

When it comes to cancer, aberrations in multiple downstream molecules involved in multiple pathways leads to deviation from normal physiology and hence multiple targets need to be knocked-out in order to establish homeostasis. So how can we solve this issue of having multiple targets being knocked-out simultaneously while having keeping off-target effects into consideration?

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edit 0 Answer 2 Views Aug 25, 2023

Please share the software and its procedure to calculate latency time Zebrafish from the recorded video in haloperidol induced catalepsy Model.
edit 0 Answer 2 Views Aug 25, 2023

Developing anti-drug resistance mechanism

studying the mechanism of drug resistance and developing anti-microbial mechanisms using CRISPR

edit 0 Answer 2 Views Aug 24, 2023

What is exactly MIC-Drop?
edit 0 Answer 2 Views Aug 23, 2023

How much helpful this technology to Academicians
edit 0 Answer 2 Views Aug 23, 2023

What is the best way to determine how efficient the knockouts are in the F0s? Do you experience genetic mosaicism in the F0s, & how to overcome this?
edit 0 Answer 2 Views Aug 22, 2023

Thank you And how I can use this technolog to control the harmful effect of bacteria
edit 0 Answer 1 View Aug 29, 2023

What is mic drop? What is it doing? How it is working?
edit 0 Answer 1 View Aug 25, 2023

Therapy of monogenic disorders using CRISPR-Cas 9 Technology

Therapy of monogenic disorders using CRISPR-Cas 9 Technology


I am curious to know about CRISPR-Cas9 Technology from the scratch. #

edit 0 Answer 1 View Aug 24, 2023

What is the most recent implementation of CRISPR in TCR or CAR T technology?
edit 0 Answer 1 View Aug 23, 2023

What is the implication of MIC-Drop platform in HIV Associated Neurocognitive disorder or NeuroAIDS field?
edit 0 Answer 1 View Aug 23, 2023

Is the technology being tried in other biological systems such as developing chicken embryos
edit 0 Answer 1 View Aug 23, 2023

Can we apply this technique in Nematodes?
edit 0 Answer 1 View Aug 23, 2023

How can we use it in our microbiolgy
edit 0 Answer 1 View Aug 23, 2023

Can use this technical in antiangegnesis for drug test?
edit 0 Answer 1 View Aug 23, 2023

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