Beyond NGS – Single-molecule genomics

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Beyond NGS – Single-molecule genomics

Speaker: Yuval Ebenstein Moderator: Alessandro Didonna

Online live: Mar 07, 2023 12:00 PM EST Posted: Apr 21, 2023 Views: 3002

Q&A

Abstract

Next generation sequencing (NGS) is revolutionizing all fields of biological research but it fails to extract the full range of information associated with genetic material. Complementary genomic technologies that analyze individual, unamplified genomic DNA are filling the gaps in the capabilities of NGS. Using such technologies we gain access to the structural variation and long range patterns of genetic and epigenetic information. Recent results from our lab demonstrate our ability to detect and map the epigenetic marks 5-methylacytosine and 5-hydroxymethylcytosine as well as various forms of DNA damage on individual genomic DNA molecules. This new technology allows genetic and epigenetic variation calling on the single cell level without the need to process single cells.

The speakers will discuss:

*Alternative approaches to studying the human genome

*Detect and map DNA damage and epigenetics marks on individual DNA Molecules

Speaker

Yuval Ebenstein, Ph.D.

Academic Director, Tel Aviv University

Next generation sequencing (NGS) is revolutionizing all fields of biological research but it fails to extract the full range of information associa...

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Moderator

Alessandro Didonna, Ph.D.

Assistant Professor, East Carolina University, USA

Dr. Didonna obtained his PhD degree in Functional and Structural Genomics from the International School for Advanced Studies (SISSA) in Italy, stud...

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Keywords

NGS, DNA damage, Epigenetics, Single molecules

References

Jeffet J, Margalit S, Michaeli Y, Ebenstein Y. Single-molecule optical genome mapping in nanochannels: multidisciplinarity at the nanoscale. Essays Biochem. 2021 Apr 16;65(1):51-66. doi: 10.1042/EBC20200021. PMID: 33739394; PMCID: PMC8056043.

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22 Q&A

How does proteomic studies help in plant biotechnology?

1 Answer 23 Views Feb 23, 2023

Surya Dev Mishra 1820115

PTMs in specific plant species helpful in understanding of overexpression of specific metabolites like plant based drug, foods and other crops productivity. Many enzymes that undergoes post translational modification and excellerate the pathways for specific productivity, environmental resistant etc. Moving towards chemical based plant proteomics helpful in tracking metabolites productivity, movement or cell stages. Nowadays many MS based technique available to screen these enzymes in cellular melieu to accelerate the plant productivity.

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