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Open-field test

BC Byung Tae Choi
DJ Da Hee Jung

Last updated date: Apr 16, 2021 Views: 1709 Forks: 0

original An abbreviated version of this protocol was published in eLife in Sep, 2020
Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
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Open-field test 

1. This take was used a black square box (60 × 60 × 30 cm3) including a center (30 × 30 cm2).

 

Note: Behavior was measured in a quiet and low-light intensity (<50 lx) room. 

2. Rat was placed in a black square box for 5 min to habituation.

Note: When placing the rat in the box, place it in the center to begin exploring (staring point=center zone).

3. After habituation, the measurement has started the box for 15 min.

4. Behavior was video-recorded for later analysis using a SMART v3.0 video tracking system (Panlab, S.L.U., Barcelona, Spain). 

5. We analyzed the total distance (cm), velocity (cm/s), and resting times in the center zone (s).

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Choi, B and Jung, D(2021). Open-field test. Bio-protocol Preprint. bio-protocol.org/prep1019.
  2. Jung, D. H., Ahn, S. M., Pak, M. E., Lee, H. J., Jung, Y. J., Kim, K. B., Shin, Y., Shin, H. K. and Choi, B. T.(2020). Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD. eLife. DOI: 10.7554/eLife.56359

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1 Q&A

Hi, I want to ask what is the right time to do this open field test? is it done in the morning, afternoon or evening?
edit 1 Answer 68 Views Mar 4, 2023
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