Materials and Reagents

1. Paper towels
2. Laboratory rats (3 months old and weight between 200 and 240 g)
   The rats are housed in groups of 4 per cage and kept in an environment with temperature (23 ± 2 °C) and humidity control. The animals have a 12-12 h light-dark cycle and food and water ad libitum.
3. Ethanol (70%) for cleaning (CID: 702)
4. Plastic sheets (PVC wall panel) (Dacheng, model: DCb-008 )
5. Adhesive glue for plastics (SG300-05)
   

Equipment

1. Home cage (Tecniplast, catalog number: 1291H001, EUROSTANDARD TYPE III H, 425 x 266 x 185 mm)
2. Classic Labyrinth for rats [a square shaped plastic enclosure (125 x 125 x 40 cm) with several labyrinth passages of identical width and height (25 x 35 cm) but variable length according to the crossing. This Labyrinth can be assembled manually as shown in Figure 1, where it is made of white plastic sheets. These plates are smooth and solid and should be easy to clean and unbreakable or damaged by the animal. The maze is formed at 125 x 125 x 40 cm by welding the plastic sheets together with adhesive glue. According to Figure 1, after the maze is made, it is placed on a wooden table 90 cm high on the ground (Figure 1)]
3. USB Camera Lenses (ITEM: 60528/ Lens for 60516, Vari-focal, 2.8-12 mm)
   Note: It was placed directly in the middle above the Labyrinth at the height of 1.20 m so that only the labyrinth was exposed.
4. Chronometer (LCD Digital Portátil Reloj Alarma Temporizador by SYMTOP)
   
   
   Figure 1. Classic Labyrinth and its dimensions

Software

1. Ethovision video tracking system software (version 10, Noldus Information Technology, France)
2. Microsoft Excel (Microsoft Corp^® 2016, USA)
3. Minitab (Minitab^® version 17.1, USA)
   

Procedure