Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed
tooltip

Mouse Model of Dextran Sodium Sulfate (DSS)-induced Colitis

葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠模型

Srustidhar Das Srustidhar Das
SB Surinder K. Batra
SR Satyanarayana Rachagani

发布: 2017年08月20日第7卷第16期 DOI: 10.21769/BioProtoc.2515 浏览次数: 21247

评审: Pasquale PellegriniAnonymous reviewer(s)

download pdf PDF PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Oncogene, featuring study using the protocol.

May 2016

Advertisement

Technologies for RNA Detection
Special Issue on Protocols for Organoid Research
“科研可重复性与创新”系列网络研讨会
Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

相关实验方案

采用快速10分钟硝酸银染色法观察骨细胞陷窝-小管系统

采用快速10分钟硝酸银染色法观察骨细胞陷窝-小管系统

Jinlian Wu [...] Libo Wang

2026年08月05日 227 阅读

石蜡包埋乳腺癌及腋窝淋巴结人体组织样本的光学透明化与小分子标记

石蜡包埋乳腺癌及腋窝淋巴结人体组织样本的光学透明化与小分子标记

Lisa D.J. Schiffelers [...] Anna Schueth

2026年08月20日 527 阅读

基于 Ventana Discovery 平台的自动化成像流程：同步定量 FFPE 人组织中的 B 细胞、浆细胞和浆母细胞

基于 Ventana Discovery 平台的自动化成像流程：同步定量 FFPE 人组织中的 B 细胞、浆细胞和浆母细胞

Patrick S. Chang [...] Cary D. Austin

2026年09月05日 102 阅读

Abstract

Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the intestinal tract and is primarily comprised of Crohn’s disease (CD) and ulcerative colitis (UC). Several murine models that include both chemical induced and genetic models have been developed that mimic some aspects of either CD or UC. These models have been instrumental in our understanding of IBD. Of the chemical induced colitis models, dextran sodium sulfate (DSS) induced colitis model is a relatively simple and very widely used model of experimental colitis.

Keywords: Dextran sodium sulfate (DSS) (葡聚糖硫酸钠（DSS）) search Colitis (结肠炎) search Inflammatory bowel disease (IBD) (炎性肠病（IBD）) search

Background

Inflammatory bowel disease (IBD) is a complex and multifactorial disease of unknown etiology (Sartor, 2006). However, multiple factors are considered to be critical in conferring IBD susceptibility, e.g., defects in host genetics, environmental triggers, aberrant immune response against microbial and dietary antigens (Sartor, 2006). Several murine models that address specific aspects of the disease process are available (Mizoguchi, 2012). DSS induced experimental colitis is a rapid and widely used model of intestinal inflammation (Perse and Cerar, 2012). Although the exact mechanism of DSS induced colitis is not well understood, it is widely accepted that disruption of the epithelial monolayer resulting in exposure of the underlying immune system to the intestinal contents containing microbiota and microbial products (Perse and Cerar, 2012). DSS induced colonic inflammation can be adapted as acute, chronic or relapsing model of intestinal inflammation by changing the concentration, duration and cycles of administration of DSS in drinking water. In this protocol, we provide a detailed description of procedures, important considerations when performing the protocol. We have used this protocol to address the role of Muc4 in DSS induced colitis in our previous work (Das et al., 2015).

Materials and Reagents

  1. Sterile Eppendorf tubes (National Scientific, catalog number: CN1700-BP )
  2. Tissue Path Macrosette processing cassettes (Fisher Scientific, catalog number: 15-182-706 )
  3. Glass slides
  4. 8-10 week old mice generated in house or obtained from the commercial vendors
    Note: Mice used in the study of Das et al., (2015) were of 129/Sv and C57BL/6J mixed background. Mice in this study were littermates and included both males and females housed under specific pathogen free (SPF) conditions.
  5. Dextran sodium sulfate (DSS) (TdB Consultancy, catalog number: DB001 , molecular weight: 35-55 kDa)
    Note: Stored in a dry place at room temperature.
  6. Liquid nitrogen
  7. Autoclaved drinking water
  8. Anesthesia (Isofluorane or CO2)
  9. 10% buffered formalin (Fisher Scientific, catalog number: SF100-4 )
  10. Ethanol
  11. Xylene
  12. Paraffin
  13. mirVana miRNA isolation kit (Thermo Fisher Scientific, InvitrogenTM, catalog number: AM1560 )
  14. Bioanalyzer (Agilent Technologies, Waldbronn, Germany)
  15. DNase I (QIAGEN, catalog number: 79254 )
  16. Oligo-dT or random hexamer primers (Thermo Fisher Scientific, InvitrogenTM, catalog number: 100023441 )
  17. SuperScript reverse transcriptase II (Thermo Fisher Scientific, InvitrogenTM, catalog number: 18064014 )
  18. Light Cycler 480 SYBR Green mix (Roche Molecular Systems, catalog number: 04707516001 )
  19. Hematoxylin and eosin (H & E)

Equipment

  1. Measuring scale/Ruler or Vernier calipers
  2. Animal weighing balance
  3. Feeding bottles
  4. Tissue-Tek VIP processing machine
  5. Dissection equipment
  6. Mouse microisolator chambers
  7. Pestle and mortar

Procedure

English
中文翻译

文章信息

版权信息

© 2017 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Das, S., Batra, S. K. and Rachagani, S. (2017). Mouse Model of Dextran Sodium Sulfate (DSS)-induced Colitis. Bio-protocol 7(16): e2515. DOI: 10.21769/BioProtoc.2515.

ris ris Download Citation in RIS Format

分类

癌症生物学 > 炎症 > 动物模型

细胞生物学 > 组织分析 > 组织染色

您对这篇实验方案有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

post 发布问题
0 Q&A
pen 提交稿件