1-MCP (1-methylcyclopropene) Treatment Protocol for Fruit or Vegetables
水果或蔬菜的1-MCP（1-甲基环丙烯）处理实验方案
发布: 2017年05月20日第7卷第10期 DOI: 10.21769/BioProtoc.2278 浏览次数: 15784
评审: Arsalan DaudiWenrong HeSimab Kanwal
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参见作者原研究论文
The authors used this protocol in:
Mar 2016
Abstract
1-MCP (1-methylcyclopropene) is a simple synthetic hydrocarbon molecule that interacts with the ethylene receptor and inhibits the response of fruit or plant to ethylene. 1-MCP has opened new opportunities in handling harvested crops and serves as a powerful tool to learn about plant response to ethylene (Watkins and Miller, 2006). 1-MCP is manufactured by Agrofresh and known by its commercial name SmartfreshSM.
Keywords: Ripening inhibition (成熟抑制)
Ethylene (乙烯)
Fruit (水果)
Postharvest (采后)
Storage (存储)
Background
Application of 1-MCP can serve as a powerful tool to examine the response of the plant to ethylene. The commercial production of 1-MCP by Agrofresh allows simple practice of this treatment, especially postharvest. The application of 1-MCP to plants, fruit or vegetable was mentioned in many articles but was not described in detail. This was the motivation to provide the following protocol. In this protocol tomatoes served as candidates for the treatment.
Materials and Reagents
- Hand gloves (latex or nitrile)
- Rubber septa (for the volumetric flask) (Sigma-Aldrich, catalog number: Z553964 )
- Syringe 10 ml (OMG, catalog number: OMG-W-10M )
- Needle 0.60 x 30 mm (Pic, catalog number: 03.070140.300.800 )
- Weighing papers for the 1-MCP powder (Whatman, catalog number: 28414662 )
- Freshly harvested tomatoes (any type and origin)
- 1-MCP powder (Agrofresh Inc.) containing 0.14% 1-MCP as active ingredient
- Tap water
Equipment
- 30 L airtight HDPE plastic barrel wide mouth and cover with lid equipped with flexible latex hose or rubber septa (Chen Samuel chemicals, catalog number: 0100230 )
- Brand volumetric flask 1,000 ml (Sigma-Aldrich, catalog number: Z326828 )
- Benchtop weight scales with 0.01 g resolution (Vibra, catalog number: AJ-3200CE )
Procedure
文章信息
版权信息
© 2017 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Dan, G., Martin, G., Yosi, S., Dario, B. and Asaph, A. (2017). 1-MCP (1-methylcyclopropene) Treatment Protocol for Fruit or Vegetables. Bio-protocol 7(10): e2278. DOI: 10.21769/BioProtoc.2278.
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分类
植物科学 > 植物新陈代谢 > 其它化合物
细胞生物学 > 细胞信号传导 > 发育
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