外周神经损伤：神经性疼痛小鼠模型

Takahiro  Masuda; Yuta  Kohro; Kazuhide  Inoue; Makoto  Tsuda

doi:10.21769/BioProtoc.2252

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Peer-reviewed

Peripheral Nerve Injury: a Mouse Model of Neuropathic Pain

外周神经损伤：神经性疼痛小鼠模型

TM Takahiro Masuda

YK Yuta Kohro

KI Kazuhide Inoue

MT Makoto Tsuda email

发布: 2017年05月05日第7卷第9期 DOI: 10.21769/BioProtoc.2252 浏览次数: 11902

评审: Andrea PuharHélène M. LégerAnonymous reviewer(s)

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Cover of Nature Communications, featuring study using the protocol.

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Abstract

Neuropathic pain is one of the highly debilitating chronic pain conditions, for which, currently, there is no therapeutic treatment. In order to reveal the underlying mechanism for neuropathic pain, various animal models have been established (Burma et al., 2016). This protocol describes how to prepare spinal nerve injury model (Kim and Chung, 1992; Rigaud et al., 2008; Masuda et al., 2016), one of the most frequently-used and highly reproducible models in which multiple alterations occur both in the peripheral and central nervous system.

Keywords: Neuropathic pain (神经性疼痛)

Nerve injury (神经损伤)

Spinal cord (脊髓)

Mouse (小鼠)

Dorsal root ganglion (DRG) (背根神经节（DRG）)

Allodynia (触诱发痛)

Background

Revealing the underlying mechanism of neuropathic pain is necessary to develop effective therapy for its optimal management. Therefore, various animal models for neuropathic pain have been developed. In particularly, rodent models have been frequently used because they are highly reproducible and exhibit pain hypersensitivity that is also observed in patients with neuropathic pain. In this protocol, we describe how to prepare the mouse spinal nerve injury model.

Materials and Reagents

5-0 silk suture (Alfresa Pharma, catalog number: GA05SW )
Sterile scalpel blades (FATHER Safety Razor, catalog number: No.10 )
Wild-type C57BL/6 mice (6-15 weeks old) (Japan Clea)
Isoflurane (Mylan)
75 % ethanol (Takasugi Pharmaceutical)

Equipment

Electric clippers (Daito Electric Machine Industry, catalog number: 605AD , Special C)
Note: This product has been discontinued.
Agricola retractor (Fine Science Tools, catalog number: 17005-04 )
Forceps (NATSUME SEISAKUSHO, catalog number: A-14 )
Vannas spring scissors (Fine Science Tools, catalog number: 15070-08 )
Electric drill (URAWA Kogyo, model: MINITORJET UC210 )
Scalpel holder (NATSUME SEISAKUSHO, catalog number: D-11 )
Heating pad (VIVARIA, catalog number: MP-916-NV)
Double sided micro spatula (Fine Science Tools, catalog number: 10091-12 )
Isoflurane dispenser (FORWICK, MURACO Medical)

Procedure

English

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如何引用

Masuda, T., Kohro, Y., Inoue, K. and Tsuda, M. (2017). Peripheral Nerve Injury: a Mouse Model of Neuropathic Pain. Bio-protocol 7(9): e2252. DOI: 10.21769/BioProtoc.2252.