高脂饮食诱导的大鼠乳腺癌模型

Meng Ju Wu; Chun Ju Chang

doi:10.21769/BioProtoc.1852

Improve Research Reproducibility A Bio-protocol resource

提交稿件
订阅
CN
- EN - English
- CN - 中文

Peer-reviewed

High Fat Diet-induced Breast Cancer Model in Rat

高脂饮食诱导的大鼠乳腺癌模型

Meng Ju Wu

CC Chun Ju Chang email

发布: 2016年07月05日第6卷第13期 DOI: 10.21769/BioProtoc.1852 浏览次数: 8059

评审: Masahiro MoritaPooja MehtaAnonymous reviewer(s)

下载 PDF

Q&A

引用

Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Cancer Research, featuring study using the protocol.

Jun 2015

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

相关实验方案

患者来源的子宫内膜癌类器官的构建与维持

Mali Barbi [...] Semir Beyaz

2024年10月20日 1072 阅读

小鼠时间与空间特异性控制的前列腺癌模型

Sen Liu [...] Qiuyang Zhang

2025年01月05日 1560 阅读

优化的患者来源类器官与匹配癌相关成纤维细胞同步培养方案

Jenny M. Högström and Taru Muranen

2025年01月20日 3081 阅读

Abstract

Obesity has been linked to breast cancer progression but the underlying mechanisms remain obscure. Being overweight or obese for a woman at the time she is diagnosed with breast cancer is linked to a high risk of recurrence regardless of treatment factors. In rodents, high body weight is also associated with increased incidence of spontaneous and chemically induced tumors. To study the complex interaction between the mammary epithelia and the microenvironment, with a focus on the mechanism underlying the role obesity plays in the regulation of the cancer stem cell traits and the development of mammary cancer in vivo, we have established a diet-induced obesity (DIO) rat model of breast cancer (Chang et al., 2015).

Keywords: Obesity (肥胖)

High-fat diet (高脂饮食)

Breast cancer (乳腺癌)

Materials and Reagents

40 µm cell strainer (STEMCELL Technologies, catalog number: 27305 )
Female Sprague-Dawley rats (Envigo, Harlan Laboratories, catalog number: Sprague Dawley^® SD^®)
1 ml, 26 G x 3/8 in. BD Tuberculin syringe with detachable needle, slip tip (BD Biosciences, catalog number: 309625 )
N-methylnitrosourea (Sigma-Aldrich, catalog number: N1517 )
Sodium chloride (Sigma-Aldrich, catalog number: S7653 )
10% neutral formalin (Sigma-Aldrich, catalog number: HT501128 )
EpiCult^TM-B Mouse Medium Kit (STEMCELL Technologies, catalog number: 05610 )
10x Gentle Collagenase/Hyaluronidase (STEMCELL Technologies, catalog number: 07919 )
Hanks’ balanced salt solution modified (HBSS) (STEMCELL Technologies, catalog number: 37150 )
Trypsin-EDTA
Ammonium chloride (NH₄Cl) (STEMCELL Technologies, catalog number: 07800 )
40 µm cell strainer (STEMCELL Technologies, catalog number: 27305)
Fetal bovine serum (FBS) (Corning, catalog number: 35-011-CV )
Flow Cytometry Stain Buffer (BD Biosciences, catalog number: 554656 )
FITC-conjugated anti-OBR antibody (aa956-986, polyclonal antibody) (Lifespan Biosciences, catalog number: LS-C261832 )
Regular rat chow diet (Envigo, catalog number: 8604 ) (see Recipes)
Western diet (Research Diets, catalog number: D12079B ) (see Recipes)
0.9% saline (see Recipes)
N-methylnitrosourea solution (see Recipes)