1. Isolation by thermal induced migration-allows for the recovery of viable larvae but not dead or moribund larvae.
2. Isolation by digestion of the lung-allows for the recovery of both dead and viable larvae.
3. Isolation by bronchoalveolar lavage (BAL) -allows for the assessment of the number of molt 4 larvae on their way to the gut.
   These protocols can be used to follow the dynamics of worm migration during infection and the effect of the host immune system on worm viability and fecundity.
   
   

Part I. Isolation by migration

Materials and Reagents

1. Cheesecloth cut into 8 x 8 cm squares (any fabric store)
   
2. 8 x 8 cm plastic disposable weigh boats (Medi'Ray-Laboratory, Runlab Archives, catalog number: RL33102 )
   
3. 50 ml centrifuge tubes (Thermo Fisher Scientific, Falcon^TM, catalog number: 352070 / 14-432-22 ) and corresponding racks
   
4. Disposable Pasteur pipettes (Interab Supply, catalog number: KJ619-1 )
   
5. 60 x 15 mm TC dish (Thermo Fisher Scientific, Falcon^TM, catalog number: 353002 / 08-772B )
   
6. N. brasiliensis infected mice (Camberis et al., 2003)
   
7. 70% Ethanol
   
8. PBS (DPBS, no calcium, no magnesium) (Thermo Fisher Scientific, Gibco^TM, catalog number: 14190-144 )
   

Equipment

1. Iris scissors (Medicon, catalog number: 202.20.11 )
   
2. Forceps (Medicon, catalog number 07.55.20 )
   
3. Water-bath set at 37 °C (Biolab, model: Grant SUB Aqua 18 ) with water set to a depth not exceeding 2 cm from the top of the 50 ml tubes
   
4. Stereo-microscope (Olympus, model: SZX16 )
   

Procedure