Measurement of Resting Energy Metabolism in Mice Using Oxymax Open Circuit Indirect Calorimeter
采用Oxymax 开放环路间接能量测定仪测量小鼠中的静息能量新陈代谢
发布: 2015年09月20日第5卷第18期 DOI: 10.21769/BioProtoc.1602 浏览次数: 10888
评审: Xuecai GeAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Aug 2014
Abstract
Indirect calorimeter is a powerful tool to monitor resting energy metabolism through the measurement of oxygen (O2) consumption and carbon dioxide (CO2) production. From the measurement of VO2 and VCO2, the respiratory exchange ratio (RER) can be calculated to assess energy fuel utilization and energy expenditure (Evan et al., 2012). Previously, indirect calorimeter has been widely used in metabolic disease research in mice to reveal the potential roles of specific genes or treatments in regulating energy metabolism (for example: Bi et al., 2014; Feng et al., 2014). Here, we described a protocol to evaluate the resting energy metabolism of C57BL/6 mice during dark and light cycles using the Oxymax Open Circuit indirect calorimeter.
Keywords: Brown adipose tissue (棕色脂肪组织)
Skeletal muscle (骨骼肌)
Oxygen consumption (耗氧)
Insulin resistance (胰岛素抵抗)
Diabetes (糖尿病)
Materials and Reagents
- Adult mice (C57BL/6 male mice at 3-month old were used for data acquisition in this protocol, but male or female mice of other genetic backgrounds or strains, at different ages can be used)
- Food (normal chow diet or high fat diet) and water (ad lib)
- Compressed gas mixture with the components of 4,929 PPM CO2, 20.47% O2 and Balance N2
Equipment
- Oxymax Open Circuit Indirect Calorimeter (Columbus Instruments, model: Open Circuit Indirect Calorimeter) (Figure 1)
Figure 1. Open circuit indirect calorimeter components - Computer with software provided by the manufacture (Columbus Instruments, model: Oxymax v4.91 )
Procedure
文章信息
版权信息
© 2015 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Nie, Y., Gavin, T. P. and Kuang, S. (2015). Measurement of Resting Energy Metabolism in Mice Using Oxymax Open Circuit Indirect Calorimeter. Bio-protocol 5(18): e1602. DOI: 10.21769/BioProtoc.1602.
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分类
细胞生物学 > 细胞新陈代谢 > 其它化合物
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