Materials and Reagents

1. Reporter line seeds (this protocol was developed using luciferase under control of a minimal promoter containing four copies of the rapid stress response element - 4xRSRE:LUCIFERASE)
   
2. Murashige and Skoog basal medium (Sigma-Aldrich, catalog number: M0404 )
   
3. Phytoagar (PlantMedia, catalog number: 40100072-2 )
   
4. Sterile disposable reagent reservoir (Corning, catalog number: 07-200-128 )
   
5. Bleach (Clorox concentrated, 8.25% sodium hypochlorite)
   
6. Hydrochloric acid (HCl)
   
7. Chemical library (source may vary)
   
8. Potassium luciferin (Gold Biotechnology, model: LUCK-1G )
   
9. ¼ strength MS media (see Recipes)
   
10. 1 mM luciferin (see Recipes)
    

Equipment

1. Micropore surgical tape (3M, model: 1530-0 )
   
2. 1.5 ml tubes (SealRite, catalog number: 1615-5500 )
   
3. 96 well plates with lid: Flat bottom, sterile but not tissue culture treated (SARSTEDT AG, catalog number: 82.1571.001 )
   
4. Filter paper (here used Whatman 1440 125, pore size does not matter)
   
5. Toothpicks
   
6. Laminar flow hood or biosafety cabinet (here used SterilGARD ii, the Baker Company)
   
7. Chemical fume hood (here used St. Charles)
   
8. Vacuum chamber
   
9. Multichannel pipette (2-20 and 20-200 µl) (Rainin)
   
10. Temperature-controlled growth cabinet (here used Conviron, model: GR48 )
    
11. Charge-Couple Device camera (CCD camera; Andor DU434-BV CCD)
    
12. Dehesh lab Perl scripts (http://www-plb.ucdavis.edu/labs/dehesh/dehesh-lab-code.html)
    

Software

1. ANDOR Solis analysis software (Andor technology, v15, http://www.andor.com/scientific-software)
   
2. ImageJ image analysis software (http://imagej.nih.gov/ij/)
   

Procedure