Materials and Reagents

1. Purified protein with chitin binding affinity
   Note: We used histidine-tagged chitin binding protein CbpD. The protein was purified by affinity chromatography onto a 5-ml HisTrap nickel column (Pharmacia) on an Äkta system (Amersham Biosciences). The complete purification protocol is described in details in Cadoret et al. (2014).
   
2. Chitin beads (New England Biolabs, catalog number: S6651 )
   
3. Tris Base
   
4. EDTA
   
5. NaCl
   
6. Tween 100
   
7. Freshly made solution of chitin binding buffer (see Recipes)
   

Equipment

1. Laboratory vortex adapted to Eppendorf tubes
   
2. Laboratory rotating wheel adapted to Eppendorf tubes
   
3. Cold room or refrigerated incubator (4 °C)
   

Procedure