Materials and Reagents

1. L. campestre seeds
   
2. Agrobacterium strain GV3101 (carrying a binary vector for plant transformation including resistance gene for bacterial selection and a T-DNA containing a Basta-resistance gene)
   
3. Seedling substrate (60% white peat; 20% frozen black peat; 20% coconut pulp) (Klasmann-Deilmann GmbH, recipe number: 080 )
   
4. Osmocote mini (The Scotts Company)
   
5. Triabon (COMPO)
   
6. Beef extract
   
7. Yeast extract
   
8. Peptone
   
9. Sucrose
   
10. MgSO₄
    
11. Rifampicin (chromosomal resistance)
    
12. Gentamycin (helper plasmid resistance)
    
13. Further antibiotics depending on the transformation vector
    
14. Silwet L-77 (Lehle Seeds, catalog number: VIS-02 )
    
15. 0.01% basta solution (Bayer CropScience GmbH, catalog number: 79011725 )
    
16. Soil (see Recipes)
    
17. YEB Medium (see Recipes)
    
18. Infiltration medium (see Recipes)
    

Equipment

1. Greenhouse for plant cultivation
   
2. Cold-room for plant vernalization
   
3. Vermiculite (1-2 mm)
   
4. 28 °C incubator with shaking
   
5. Beaker
   
6. Centrifuge with temperature control
   
7. Spectrophotometer
   
8. Magnetic stirrer
   
9. Small plastic bags
   

Procedure