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RcGTA, a small phage-like particle produced by Rhodobacter capsulatus, was initially identified in culture filtrates as a DNase-resistant form of DNA transfer between R. capsulatus cells. This gene transfer assay has been used to identify RcGTA-producing strains and to help determine the roles of genes thought to be responsible for RcGTA production.
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APH was supported by fellowships from Memorial University School of Graduate Studies and the Natural Sciences and Engineering Research Council (NSERC) of Canada. This research in ASL’s lab was supported by grants from NSERC, the Canada Foundation for Innovation, and the Newfoundland and Labrador Research & Development Corporation. This protocol is based on work originally reported in: Hynes et al. (2012).
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