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Scratch Wound Healing Assay has been widely adapted and modified by researchers to study the effects of a variety of experimental conditions, for instance, gene-knockdown or chemical compound treatment, on cell migration and proliferation. The assay is simple and inexpensive, and the experimental conditions can be easily modified for different purposes. The basic principle of the assay is that, a “wound gap” in a cell monolayer is created by scratch, followed by monitoring the “healing” of this gap by cell migrating and growth towards the center of the gap, hereby filling up the “gap”. Factors that alter the motility and/or growth of the cell can lead to increased or decreased rate of “healing” of the gap (1). The assay is quantitative, and is suitable for high-throughput screen if antomated system is used (2).
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