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Yeast Genomic DNA Miniprep Using A FastPrep Cell Lyser
Published: May 5, 2011 DOI: 10.21769/BioProtoc.64 Views: 11972
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Abstract
This method is a convenient way to purify high-quality genomic DNA from yeast cells. It is suitable for PCR and other assays that require genomic DNA of higher quality.
Keywords: Genomic DNA
Yeast
Small scale
FastPrep Cell Lyser
PCR
Materials and Reagents
- 5 M Ammonium acetate (pH 7.0)
- Chloroform
- Isopropanol
- 70% Ethanol
- Lysis buffer (see Recipes)
Equipment
- Adapted for Fastprep machine
- Screw-tube
- Glass beads
- Microfuge
Procedure
Copyright: © 2011 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > DNA extraction
Microbiology > Microbial genetics > DNA
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