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Volume:3 Issue:22
November 20, 2013
Cell Biology
Harvest and Culture of Mouse Peritoneal Macrophages
Authors:
Mingfang Lu
Mingfang Lu
Affiliation:
Internal Medicine, University of Texas Southwestern Medical School, Dallas, TX, USA
Bio-protocol author page:
a990
and
Alan W. Varley
Alan W. Varley
Affiliation:
Internal Medicine, University of Texas Southwestern Medical School, Dallas, TX, USA
For correspondence:
alanwaynevarley@gmail.com
Bio-protocol author page:
a991
,
date: 11/20/2013, 13135 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.976
[Abstract] Peritoneal macrophages are used as primary macrophages in lots of studies, mainly because they are easy to obtain. Injection of thioglycollate broth i.p. induces inflammatory responses and elicits large numbers of macrophages. This protocol can be used ...
Article identifier: bio-protocol.org/e976
Immunology
Gastric Aspiration Models
Authors:
Bruce A. Davidson
Bruce A. Davidson
Affiliation:
Department of Anesthesiology, and Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, USA
For correspondence:
bdavidso@buffalo.edu
Bio-protocol author page:
a1019
and
Ravi Alluri
Ravi Alluri
Affiliation:
Department of Anesthesiology, and Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, USA
Bio-protocol author page:
a1020
,
date: 11/20/2013, 7446 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.968
[Abstract] The procedures described below are for producing gastric aspiration pneumonitis in mice with alterations for rats and rabbits described parenthetically. We use 4 different injury vehicles delivered intratracheally to investigate the inflammatory responses ...
Article identifier: bio-protocol.org/e968
Immunocytochemical Detection of Recombinant Biomphalysin on
Schistosoma mansoni
Sporocysts
Authors:
David Duval
David Duval
Affiliation:
Ecologie et Evolution des Interactions (2EI), UPVD, CNRS, UMR 5244, Perpignan, France
For correspondence:
david.duval@univ-perp.fr
Bio-protocol author page:
a1014
,
Richard Galinier
Richard Galinier
Affiliation:
Ecologie et Evolution des Interactions (2EI), UPVD, CNRS, UMR 5244, Perpignan, France
Bio-protocol author page:
a1015
,
Julien Portela
Julien Portela
Affiliation:
Ecologie et Evolution des Interactions (2EI), UPVD, CNRS, UMR 5244, Perpignan, France
Bio-protocol author page:
a1016
,
Guillaume Mitta
Guillaume Mitta
Affiliation:
Ecologie et Evolution des Interactions (2EI), UPVD, CNRS, UMR 5244, Perpignan, France
Bio-protocol author page:
a1017
and
Benjamin Gourbal
Benjamin Gourbal
Affiliation:
Ecologie et Evolution des Interactions (2EI), UPVD, CNRS, UMR 5244, Perpignan, France
Bio-protocol author page:
a1018
,
date: 11/20/2013, 2903 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.969
[Abstract] Schistosomiasis, or bilharzia, is a tropical disease caused by worms of the genus
Schistosoma
which infect about 200 million people. The life cycle of the parasite requires
Biomphalaria
, a specific genus of freshwater snails, as intermediate. Using an ...
Article identifier: bio-protocol.org/e969
Assessment of Human Dendritic Cell Antigen Uptake by Flow Cytometry
Authors:
Ana Luque
Ana Luque
Affiliation:
Human Molecular Genetics Department, IDIBELL, L'Hospitalet de Llobregat, Spain
Bio-protocol author page:
a995
,
Sonia Cárdenas-Brito
Sonia Cárdenas-Brito
Affiliation:
Human Molecular Genetics Department, IDIBELL, L'Hospitalet de Llobregat, Spain
Bio-protocol author page:
a996
,
Rut Olivar
Rut Olivar
Affiliation:
Human Molecular Genetics Department, IDIBELL, L'Hospitalet de Llobregat, Spain
Bio-protocol author page:
a997
and
Josep M. Aran
Josep M. Aran
Affiliation:
Human Molecular Genetics Department, IDIBELL, L'Hospitalet de Llobregat, Spain
For correspondence:
jaran@idibell.cat
Bio-protocol author page:
a998
,
date: 11/20/2013, 4766 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.974
[Abstract] Antigen uptake by dendritic cells is the first key step towards induction of antigen-specific T-cell responses. This flow cytometry-based protocol describes the analysis of dendritic cell uptake of soluble antigens through two different mechanisms: non-specific ...
Article identifier: bio-protocol.org/e974
Microbiology
Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a
Bordetella pertussis
Response Regulator,
in vivo
and
in vitro
Authors:
Qing Chen
Qing Chen
Affiliation:
Center For Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA
Bio-protocol author page:
a1010
,
Alice Boulanger
Alice Boulanger
Affiliation:
NIDDK, National Institutes of Health, Bethesda, MD, USA
Bio-protocol author page:
a1011
,
Deborah M. Hinton
Deborah M. Hinton
Affiliation:
NIDDK, National Institutes of Health, Bethesda, MD, USA
For correspondence:
dhinton@helix.nih.gov
Bio-protocol author page:
a1012
and
Scott Stibitz
Scott Stibitz
Affiliation:
Center For Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA
Bio-protocol author page:
a1013
,
date: 11/20/2013, 4224 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.970
[Abstract] Protein phosphorylation plays a central role in signal transduction in bacteria. However, separation and detection of the phosphorylated protein from its nonphosphorylated form remain challenging. Here we describe a method to detect phosphorylation of ...
Article identifier: bio-protocol.org/e970
Transport Assays in
Aspergillus nidulans
Authors:
Emilia Krypotou
Emilia Krypotou
Affiliation:
Faculty of Biology, University of Athens, Athens, Greece
Bio-protocol author page:
a1008
and
George Diallinas
George Diallinas
Affiliation:
Faculty of Biology, University of Athens, Athens, Greece
For correspondence:
diallina@biol.uoa.gr
Bio-protocol author page:
a1009
,
date: 11/20/2013, 2708 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.971
[Abstract] Transport assays allow the direct kinetic analysis of a specific transporter by measuring apparent
K
m
and
V
max
values, and permit the characterization of substrate specificity profiles through competition assays. In this protocol, we describe a rapid ...
Article identifier: bio-protocol.org/e971
Shigella
IpaD and IpaB Surface Localizations
Authors:
Lionel Schiavolin
Lionel Schiavolin
Affiliation:
Molecular Bacteriology Lab, Université Libre de Bruxelles, Anderlecht, Belgium
For correspondence:
lschiavo@ulb.ac.be
Bio-protocol author page:
a992
,
Alaeddine Meghraoui
Alaeddine Meghraoui
Affiliation:
Molecular Bacteriology Lab, Université Libre de Bruxelles, Anderlecht, Belgium
Bio-protocol author page:
a993
and
Abdelmounnaim Allaoui
Abdelmounnaim Allaoui
Affiliation:
Molecular Bacteriology Lab, Université Libre de Bruxelles, Anderlecht, Belgium
Bio-protocol author page:
a994
,
date: 11/20/2013, 3459 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.975
[Abstract]
Shigella
uses a type III secretion system to invade host cell and to cause disease. Secretion control and insertion of a translocation pore into cell membrane are critical steps for pathogenesis and are tightly linked to the formation of the needle tip ...
Article identifier: bio-protocol.org/e975
Assay to Evaluate Vascular Permeability Induction in Mice
Authors:
Henry Puerta-Guardo
Henry Puerta-Guardo
Affiliation:
Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a980
,
Arturo Raya-Sandino
Arturo Raya-Sandino
Affiliation:
Department of Physiolology, Biophysics and Neurosciences, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a981
,
Lorenza González-Mariscal
Lorenza González-Mariscal
Affiliation:
Department of Physiolology, Biophysics and Neurosciences, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a982
,
Victor H. Rosales
Victor H. Rosales
Affiliation:
General Services Laboratory, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a983
,
José Ayala-Dávila
José Ayala-Dávila
Affiliation:
Department of Physiolology, Biophysics and Neurosciences, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a984
,
Bibiana Chávez-Mungía
Bibiana Chávez-Mungía
Affiliation:
Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a985
,
Daniel Martínez-Fong
Daniel Martínez-Fong
Affiliation:
Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a986
,
Fernando Medina
Fernando Medina
Affiliation:
Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
Bio-protocol author page:
a987
,
Juan E. Ludert
Juan E. Ludert
Affiliation:
Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
For correspondence:
jludert@cinvestav.mx
Bio-protocol author page:
a988
and
Rosa María del Angel
Rosa María del Angel
Affiliation:
Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, Instituto Politécnico Nacional (CINVESTAV-IPN), Mexico City, México
For correspondence:
rmangel@cinvestav.mx
Bio-protocol author page:
a989
,
date: 11/20/2013, 9127 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.977
[Abstract] Dengue virus infection usually courses as a benign self-limited fever, called dengue fever. However, on occasions it can progress to a life-threatening complication known as severe dengue (SD). A hallmark of SD is a sharp increase in vascular permeability. ...
Article identifier: bio-protocol.org/e977
Neuroscience
Cell Cycle Analysis in the Vertebrate Brain Using Immunolabeled Fresh Cell Nuclei
Authors:
Noelia Lopez-Sanchez
Noelia Lopez-Sanchez
Affiliation:
Molecular, Cellular and Developmental Neurobiology, Cajal Institute, CSIC, Madrid, Spain
Bio-protocol author page:
a999
and
Jose M. Frade
Jose M. Frade
Affiliation:
Molecular, Cellular and Developmental Neurobiology, Cajal Institute, CSIC, Madrid, Spain
For correspondence:
frade@cajal.csic.es
Bio-protocol author page:
a1000
,
date: 11/20/2013, 7123 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.973
[Abstract] Flow cytometry, a standard technique used for quantitative analysis of isolated cells, is routinely employed by immunologists and oncologists to study DNA content, protein expression, and other functional parameters in blood and tumor cells. Unfortunately, ...
Article identifier: bio-protocol.org/e973
Plant Science
Mapping and Analysis of Illumina Reads for Transcriptome of
Medicago Truncatula
During the Early Organogenesis of the Nodule
Authors:
Alexandre Boscari
Alexandre Boscari
Affiliation:
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1355, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7254, and Université de Nice-Sophia Antipolis, Unité Mixte de Recherche Institut Sophia Agrobiotech, Sophia Antipolis, France
For correspondence:
alexandre.boscari@sophia.inra.fr
Bio-protocol author page:
a504
,
Alberto Ferrarini
Alberto Ferrarini
Affiliation:
Università degli Studi di Verona, Dipartimento di Biotecnologie, Centro di Genomica Funzionale, Verona, Italy
Bio-protocol author page:
a1025
,
Jennifer del Giudice
Jennifer del Giudice
Affiliation:
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1355, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7254, and Université de Nice-Sophia Antipolis, Unité Mixte de Recherche Institut Sophia Agrobiotech, Sophia Antipolis, France
Bio-protocol author page:
a1026
,
Luca Venturini
Luca Venturini
Affiliation:
Università degli Studi di Verona, Dipartimento di Biotecnologie, Centro di Genomica Funzionale, Verona, Italy
Bio-protocol author page:
a1027
,
Massimo Delledone
Massimo Delledone
Affiliation:
Università degli Studi di Verona, Dipartimento di Biotecnologie, Centro di Genomica Funzionale, Verona, Italy
Bio-protocol author page:
a1028
and
Alain Puppo
Alain Puppo
Affiliation:
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1355, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7254, and Université de Nice-Sophia Antipolis, Unité Mixte de Recherche Institut Sophia Agrobiotech, Sophia Antipolis, France
For correspondence:
puppo@unice.fr
Bio-protocol author page:
a1029
,
date: 11/20/2013, 4214 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.966
[Abstract]
Medicago truncatula
serves as a model plant for legume genetics and genomics. We used RNA-Seq to characterize the transcriptome during the early organogenesis of the nodule and during its functioning. We generated approximately 135.5 million high-quality ...
Article identifier: bio-protocol.org/e966
Analysis of RNA-protein Interactions Using Electrophoretic Mobility Shift Assay (Gel Shift Assay)
Authors:
Saiprasad Goud Palusa
Saiprasad Goud Palusa
Affiliation:
Department of Biology, Colorado State University, Fort Collins, USA
Bio-protocol author page:
a1022
and
Anireddy S. N. Reddy
Anireddy S. N. Reddy
Affiliation:
Department of Biology, Colorado State University, Fort Collins, USA
For correspondence:
reddy@colostate.edu
Bio-protocol author page:
a1023
,
date: 11/20/2013, 10892 views,
4 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.967
[Abstract] RNA binding proteins (RBPs) play a crucial role in regulating gene expression at the post-transcriptional level at multiple steps including pre-mRNA splicing, polyadenylation, mRNA stability, mRNA localization and translation. RBPs regulate these processes ...
Article identifier: bio-protocol.org/e967
Cotton Ovules Culture and Analysis
Authors:
Jiafu Tan
Jiafu Tan
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Bio-protocol author page:
a1001
,
Fenglin Deng
Fenglin Deng
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Bio-protocol author page:
a1002
,
Wenxin Tang
Wenxin Tang
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Bio-protocol author page:
a1003
,
Jie Han
Jie Han
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Bio-protocol author page:
a1004
,
Guo Kai
Guo Kai
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Bio-protocol author page:
a1005
,
Lili Tu
Lili Tu
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Bio-protocol author page:
a1006
and
Xianlong Zhang
Xianlong Zhang
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
For correspondence:
xlzhang@mail.hzau.edu.cn
Bio-protocol author page:
a1007
,
date: 11/20/2013, 2873 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.972
[Abstract] The cotton ovules culture was innovated by Beasley and Ting (1973), and named after them. It is a convenient system to analyze the effect of chemical or environmental treatment on fiber development directly on ovules. This protocol was generated according ...
Article identifier: bio-protocol.org/e972
Shikimate Hydroxycinnamoyl Transferase (HCT) Activity Assays in
Populus nigra
Authors:
Igor Cesarino
Igor Cesarino
Affiliation:
Department of Plant Systems Biology / Department of Plant Biotechnology and Bioinformatics, VIB / Ghent University, Gent, Belgium
Bio-protocol author page:
a975
,
Ruben Vanholme
Ruben Vanholme
Affiliation:
Department of Plant Systems Biology / Department of Plant Biotechnology and Bioinformatics, VIB / Ghent University, Gent, Belgium
Bio-protocol author page:
a976
,
Geert Goeminne
Geert Goeminne
Affiliation:
Department of Plant Systems Biology / Department of Plant Biotechnology and Bioinformatics, VIB / Ghent University, Gent, Belgium
Bio-protocol author page:
a977
,
Bartel Vanholme
Bartel Vanholme
Affiliation:
Department of Plant Systems Biology / Department of Plant Biotechnology and Bioinformatics, VIB / Ghent University, Gent, Belgium
Bio-protocol author page:
a978
and
Wout Boerjan
Wout Boerjan
Affiliation:
Department of Plant Systems Biology / Department of Plant Biotechnology and Bioinformatics, VIB / Ghent University, Gent, Belgium
For correspondence:
wout.boerjan@psb.vib-ugent.be
Bio-protocol author page:
a979
,
date: 11/20/2013, 4557 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.978
[Abstract] Lignin is a complex phenolic polymer deposited in secondarily-thickened plant cell walls. The polymer is mainly derived from the three primary monolignols:
p
-coumaryl, coniferyl and sinapyl alcohol which give rise to p-hydroxyphenyl, guaiacyl and syringyl ...
Article identifier: bio-protocol.org/e978
Bimolecular Fluorescence Complementation (BIFC) Protocol for Rice Protoplast Transformation
Authors:
Kun Wang
Kun Wang
Affiliation 1:
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China
Affiliation 2:
Wuhan Botanical Garden, Chinese Academy of Science, Wuhan, China
Bio-protocol author page:
a973
,
Ying Liu
Ying Liu
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China
Bio-protocol author page:
a1825
and
Shaoqing Li
Shaoqing Li
Affiliation:
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China
For correspondence:
shaoqingli@whu.edu.cn
Bio-protocol author page:
a974
,
date: 11/20/2013, 4067 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.979
[Abstract] After the plant cells are removed the cell walls by digestive enzyme, the plant protoplasts still have good cell activity. The protoplasts can be used to transiently express proteins of target genes in living plant cells through polyethylene glycol (PEG) ...
Article identifier: bio-protocol.org/e979
Binding Assay of Cytosolic Proteins to the Cytoskeleton
Authors:
Stefano Del Duca
Stefano Del Duca
Affiliation:
Dipartimento di Scienze Biologiche, Geologiche ed Ambientali, University of Bologna, Bologna, Italy
For correspondence:
stefano.delduca@unibo.it
Bio-protocol author page:
a971
and
Giampiero Cai
Giampiero Cai
Affiliation:
Dipartimento Scienze della Vita, University of Siena, Siena, Italy
Bio-protocol author page:
a972
,
date: 11/20/2013, 3608 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.980
[Abstract] Many cellular proteins interact with the cytoskeleton (both actin filaments and microtubules), either dynamically or permanently. This interaction is required during different aspects of the cell life, for example during the process of cell division. ...
Article identifier: bio-protocol.org/e980
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