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Volume:3 Issue:8
April 20, 2013
Cell Biology
Flow Cytometric Detection of Mitochondrial Membrane Potential
Authors:
Hsin-Yi Chang
Hsin-Yi Chang
Affiliation:
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taipei
Bio-protocol author page:
a571
,
Hsuan-Cheng Huang
Hsuan-Cheng Huang
Affiliation:
Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan
Bio-protocol author page:
a572
,
Tsui-Chin Huang
Tsui-Chin Huang
Affiliation:
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
Bio-protocol author page:
a573
,
Pan-Chyr Yang
Pan-Chyr Yang
Affiliation:
Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan
Bio-protocol author page:
a574
,
Yi-Ching Wang
Yi-Ching Wang
Affiliation:
Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan
Bio-protocol author page:
a575
and
Hsueh-Fen Juan
Hsueh-Fen Juan
Affiliation:
Department of Life Science, Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
For correspondence:
yukijuan@ntu.edu.tw
Bio-protocol author page:
a324
,
date: 4/20/2013, 10246 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.430
[Abstract] Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function and an indicator of cell health. Depletion of Δψm suggests the loss of mitochondrial membrane integrity reflecting the initiation of the proapoptotic signal. Recently, ...
Article identifier: bio-protocol.org/e430
Flow Cytometric Detection of Reactive Oxygen Species
Authors:
Hsin-Yi Chang
Hsin-Yi Chang
Affiliation:
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
Bio-protocol author page:
a571
,
Hsuan-Cheng Huang
Hsuan-Cheng Huang
Affiliation:
Institute of Biomedical Informatics, National Yang-Ming University, Taipei, Taiwan
Bio-protocol author page:
a572
,
Tsui-Chin Huang
Tsui-Chin Huang
Affiliation:
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
Bio-protocol author page:
a573
,
Pan-Chyr Yang
Pan-Chyr Yang
Affiliation:
Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan
Bio-protocol author page:
a574
,
Yi-Ching Wang
Yi-Ching Wang
Affiliation:
Department of Pharmacology, National Cheng Kung University, Tainan, Taiwan
Bio-protocol author page:
a575
and
Hsueh-Fen Juan
Hsueh-Fen Juan
Affiliation:
Department of Life Science, Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
For correspondence:
yukijuan@ntu.edu.tw
Bio-protocol author page:
a324
,
date: 4/20/2013, 15801 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.431
[Abstract] Reactive oxygen species (ROS) are molecules containing hydroxyl radicals or peroxides with unpaired electrons. In healthy aerobic cells, ROS are produced naturally as a byproduct of oxidative phosphorylation, oxidoreductase enzymes, or metal catalyzed ...
Article identifier: bio-protocol.org/e431
Immunology
Evaluation of Caspase-1 Activation and IL-1β Production in A Kainic Acid Microdyalisis Brain Injury Model
Authors:
Antonio S. Herranz
Antonio S. Herranz
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
For correspondence:
antonio.sanchez@hrc.es
Bio-protocol author page:
a583
,
Eulalia Bazán
Eulalia Bazán
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a584
,
Diana Reimers
Diana Reimers
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a585
,
María T. Montero-Vega
María T. Montero-Vega
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a586
,
Adriano Jménez-Escrig
Adriano Jménez-Escrig
Affiliation:
Medicina y Especialidades Médicas, Universidad de Alcalá, Madrid, Spain
Bio-protocol author page:
a587
and
Pablo Pelegrín
Pablo Pelegrín
Affiliation:
Inflammation and Experimental Surgery Unit, University Clinical Hospital Virgen Arrixaca, Murcia, Spain
For correspondence:
pablo.pelegrin@ffis.es
Bio-protocol author page:
a326
,
date: 4/20/2013, 3671 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.433
[Abstract] Intracerebral infusion of kainic acid (KA) by a microdialysis probe induces a focal swelling in the brain-perfused area which promotes inflammation (Compan
et al
., 2012; Oprica
et al
., 2003). The microdialysis technique allows the local
in vivo
perfusion ...
Article identifier: bio-protocol.org/e433
Microbiology
Plasmodium falciparum
Rosette Disruption Assay
Authors:
Micheline Guillotte
Micheline Guillotte
Affiliation:
Parasite Molecular Immunology Unit, Institut Pasteur, Paris, France
For correspondence:
micheline.guillotte-blisnick@pasteur.fr
Bio-protocol author page:
a576
,
Odile Mercereau-Puijalon
Odile Mercereau-Puijalon
Affiliation:
Parasite Molecular Immunology Unit, Institut Pasteur, Paris, France
Bio-protocol author page:
a308
and
Inès Vigan-Womas
Inès Vigan-Womas
Affiliation:
Parasite Molecular Immunology Unit, Institut Pasteur, Paris, France
Bio-protocol author page:
a577
,
date: 4/20/2013, 4196 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.411
[Abstract] Rosetting,
i.e.
the capacity of
Plasmodium falciparum
-infected red blood cells (iRBCs) to bind two or more uninfected red blood cells (RBCs) is associated with severe malaria in African children. Disruption of rosettes using small soluble inhibitors ...
Article identifier: bio-protocol.org/e411
Plasmodium falciparum
Rosette Formation Assay
Authors:
Inès Vigan-Womas
Inès Vigan-Womas
Affiliation:
Parasite Molecular Immunology Unit, Institut Pasteur, Paris, France
For correspondence:
ines.vigan-womas@pasteur.fr
Bio-protocol author page:
a577
,
Micheline Guillotte
Micheline Guillotte
Affiliation:
Parasite Molecular Immunology Unit, Institut Pasteur, Paris, France
Bio-protocol author page:
a576
and
Odile Mercereau-Puijalon
Odile Mercereau-Puijalon
Affiliation:
Parasite Molecular Immunology Unit, Institut Pasteur, Paris, France
Bio-protocol author page:
a308
,
date: 4/20/2013, 5735 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.412
[Abstract] Rosetting,
i.e.
the capacity of red blood cells (iRBCs) infected with mature parasite stages to bind two or more uninfected red blood cells (RBCs) is a virulence factor of
Plasmodium falciparum
. This protocol describes an
in vitro
assay to monitor rosette ...
Article identifier: bio-protocol.org/e412
Packaging of Retroviral RNA into Viral Particles Analyzed by Quantitative Reverse Transcriptase-PCR
Authors:
Bianca Hoffmann
Bianca Hoffmann
Affiliation:
Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany
Bio-protocol author page:
a446
and
Bastian Grewe
Bastian Grewe
Affiliation:
Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany
For correspondence:
bastian.grewe@rub.de
Bio-protocol author page:
a371
,
date: 4/20/2013, 6282 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.684
[Abstract] Formation of viral particles and packaging of genomic retroviral RNA into these particles are important steps in the late phase of the viral replication cycle. The efficiency of the incorporation of viral or cellular RNAs into viral particles can be ...
Article identifier: bio-protocol.org/e684
Molecular Biology
High-throughput Method for Determination of Seed Paternity by Microsatellite Markers
Authors:
Samik Bhattacharya
Samik Bhattacharya
Affiliation:
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page:
a582
and
Ian T. Baldwin
Ian T. Baldwin
Affiliation:
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
For correspondence:
baldwin@ice.mpg.de
Bio-protocol author page:
a581
,
date: 4/20/2013, 5014 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.452
[Abstract] In this protocol, determination of seed paternity by microsatellite markers in
Nicotiana attenuata
is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a ...
Article identifier: bio-protocol.org/e452
Detection and Cloning of Spliced Transcripts by RT-PCR
Authors:
Bianca Hoffmann
Bianca Hoffmann
Affiliation:
Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany
Bio-protocol author page:
a446
and
Bastian Grewe
Bastian Grewe
Affiliation:
Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany
For correspondence:
bastian.grewe@rub.de
Bio-protocol author page:
a371
,
date: 4/20/2013, 4841 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.486
[Abstract] Using a Reverse Transcriptase-PCR approach spliced transcripts can be converted to cDNA, amplified and cloned into an expression plasmid. Sequencing of the obtained cDNA allows identification of the splicing events that generated the detected RNA (Grewe ...
Article identifier: bio-protocol.org/e486
p65 Chromatin Immunoprecipitation Protocol
Author:
Crissy Dudgeon
Crissy Dudgeon
Affiliation:
Pharmacology and Chemical Biology Department, University of Pittsburgh, Pittsburgh, USA
For correspondence:
dudgeonc@upmc.edu
Bio-protocol author page:
a570
,
date: 4/20/2013, 3931 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.683
[Abstract] Chromatin Immunoprecipitation (ChIP) is an important procedure that allows you to verify if a certain protein is physically located at a regulatory region. This information, taken together with other procedures such as luciferase assays and EMSAs, will ...
Article identifier: bio-protocol.org/e683
Neuroscience
Primary Culture of Cortical Neurons
Authors:
Rieko Muramatsu
Rieko Muramatsu
Affiliation:
Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, Suita, Japan
For correspondence:
muramatsu@molneu.med.osaka-u.ac.jp
Bio-protocol author page:
a566
and
Toshihide Yamashita
Toshihide Yamashita
Affiliation:
Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, Suita, Japan
For correspondence:
yamashita@molneu.med.osaka-u.ac.jp
Bio-protocol author page:
a379
,
date: 4/20/2013, 7622 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.496
[Abstract] Primary culture of neurons from cerebral cortex is a popular model to study neuronal function
in vitro
and to explore the molecular mechanism of neurite outgrowth in the developing and adult central nervous system. This protocol is for preparing a culture ...
Article identifier: bio-protocol.org/e496
Culture of Rat Olfactory Ensheathing Cells Using EasySep® Magnetic Nanoparticle Separation
Authors:
Susan Louise Lindsay
Susan Louise Lindsay
Affiliation:
Glial Cell Biology Group, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK
Bio-protocol author page:
a568
and
Susan Carol Barnett
Susan Carol Barnett
Affiliation:
Glial Cell Biology Group, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK
For correspondence:
susan.barnett@glasgow.ac.uk
Bio-protocol author page:
a372
,
date: 4/20/2013, 3339 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.682
[Abstract] Olfactory ensheathing cells (OECs) can be isolated and purified from a range of postnatal day 7-day to 10-day rat olfactory bulbs. Rat OECs express the CD271/p75NTR receptor and using the “Do-It-Yourself” magnetic nanoparticle EasySep kit from STEMCELL ...
Article identifier: bio-protocol.org/e682
Plant Science
Determination of Nectar Nicotine Concentration in
N. attenuata
Authors:
Eva Rothe
Eva Rothe
Affiliation:
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page:
a578
,
Matthias Schöttner
Matthias Schöttner
Affiliation:
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page:
a579
,
Danny Kessler
Danny Kessler
Affiliation:
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page:
a580
and
Ian T. Baldwin
Ian T. Baldwin
Affiliation:
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
For correspondence:
baldwin@ice.mpg.de
Bio-protocol author page:
a581
,
date: 4/20/2013, 3997 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.451
[Abstract] In this protocol, the determination of the nicotine concentration in nectar of
Nicotiana attenuata
is described. This method is applicable for the investigation of small amounts of nectar (above 1 μl). It is a high-throughput protocol optimized and streamlined ...
Article identifier: bio-protocol.org/e451
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