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Volume:4 Issue:21
November 05, 2014
Immunology
Binding Affinity Measurement of Antibodies from Crude Hybridoma Samples by SPR
Authors:
Dorin Mlaki Ndao
Dorin Mlaki Ndao
Affiliation:
AC Immune SA, Lausanne, Switzerland
Bio-protocol author page:
a1751
,
David T. Hickman
David T. Hickman
Affiliation:
AC Immune SA, Lausanne, Switzerland
Bio-protocol author page:
a1752
,
María Pilar López-Deber
María Pilar López-Deber
Affiliation:
AC Immune SA, Lausanne, Switzerland
Bio-protocol author page:
a1753
,
Aurélien Davranche
Aurélien Davranche
Affiliation:
AC Immune SA, Lausanne, Switzerland
Bio-protocol author page:
a1754
,
Andrea Pfeifer
Andrea Pfeifer
Affiliation:
AC Immune SA, Lausanne, Switzerland
Bio-protocol author page:
a1755
and
Andreas Muhs
Andreas Muhs
Affiliation:
AC Immune SA, Lausanne, Switzerland
For correspondence:
andreas.muhs@acimmune.com
Bio-protocol author page:
a1756
,
date: 11/5/2014, 4561 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1276
[Abstract] Surface Plasmon Resonance (SPR) is widely used to generate kinetic and affinity information on specific interactions between biomolecules. This technique is label-free and monitors the binding event in real-time. It is generally used for characterization ...
Article identifier: bio-protocol.org/e1276
Isolation and
In vitro
Activation of Mouse Peyer’s Patch Cells from Small Intestine Tissue
Authors:
Claudia Pastori
Claudia Pastori
Affiliation:
Division of Immunology, San Raffaele Scientific Institute, Milan, Italy
Bio-protocol author page:
a1763
and
Lucia Lopalco
Lucia Lopalco
Affiliation:
Division of Immunology, San Raffaele Scientific Institute, Milan, Italy
For correspondence:
lopalco.lucia@hsr.it
Bio-protocol author page:
a1764
,
date: 11/5/2014, 4463 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1282
[Abstract] The lumen of gastrointestinal tract is exposed to several potentially pathogenic microorganisms, thus it is extremely relevant to understand how immunosurveilance can be established. Peyer’s Patches (PPs) are oval or round lymphoid nodules that protrude ...
Article identifier: bio-protocol.org/e1282
Microbiology
Measurement of Proton-driven Antiport in
Escherichia coli
Authors:
Scarlett R. Holdsworth
Scarlett R. Holdsworth
Affiliation:
Institute for Global Food Security, Queen’s University Belfast, Belfast, UK
Bio-protocol author page:
a1759
and
Christopher J. Law
Christopher J. Law
Affiliation:
Institute for Global Food Security, Queen’s University Belfast, Belfast, UK
For correspondence:
c.law@qub.ac.uk
Bio-protocol author page:
a1760
,
date: 11/5/2014, 2603 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1278
[Abstract] Secondary active transport of substrates across the inner membrane is vital to the bacterial cell. Of the secondary active transporter families, the ubiquitous major facilitator superfamily (MFS) is the largest and most functionally diverse (Reddy
et ...
Article identifier: bio-protocol.org/e1278
Measurement of the Electrogenicity of Bile Salt/H
+
Antiport in
Escherichia coli
Authors:
Scarlett R. Holdsworth
Scarlett R. Holdsworth
Affiliation:
Institute for Global Food Security, Queen’s University Belfast, Belfast, UK
Bio-protocol author page:
a1759
and
Christopher J. Law
Christopher J. Law
Affiliation:
Institute for Global Food Security, Queen’s University Belfast, Belfast, UK
For correspondence:
c.law@qub.ac.uk
Bio-protocol author page:
a1760
,
date: 11/5/2014, 2205 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1279
[Abstract] The transmembrane proton gradient (ΔpH) is the primary source of energy exploited by secondary active substrate/H
+
antiporters to drive the electroneutral transport of substrates across the
Escherichia coli
(
E. coli
) inner membrane. Such electroneutral ...
Article identifier: bio-protocol.org/e1279
Purification and Detection of a PDGA Depolymerase from
Pusillimonas noertemannii
Authors:
David Negus
David Negus
Affiliation:
University College London School of Pharmacy, London, UK
For correspondence:
d.negus@ucl.ac.uk
Bio-protocol author page:
a1761
and
Peter W. Taylor
Peter W. Taylor
Affiliation:
University College London School of Pharmacy, London, UK
Bio-protocol author page:
a1762
,
date: 11/5/2014, 2464 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1280
[Abstract] The purification of a target protein from a complex mixture of proteins is a challenging undertaking. If the target protein has been previously characterised, then information such as subcellular location, function, molecular weight and pI can be used ...
Article identifier: bio-protocol.org/e1280
Accelerated Storage Stability Testing of a Potential Anti-Anthrax Therapeutic, EnvD
Authors:
David Negus
David Negus
Affiliation:
University College London School of Pharmacy, London, UK
For correspondence:
d.negus@ucl.ac.uk
Bio-protocol author page:
a1761
and
Peter W. Taylor
Peter W. Taylor
Affiliation:
University College London School of Pharmacy, London, UK
Bio-protocol author page:
a1762
,
date: 11/5/2014, 2245 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1281
[Abstract] The purpose of stability testing is to determine how the properties of a particular therapeutic vary with time under the influence of specific environmental factors. Information regarding the long-term stability of therapeutics can be extrapolated by ...
Article identifier: bio-protocol.org/e1281
Stable Transformation of Cyanobacterium
Synechocystis
sp.
Author:
Reinhard K Proels
Reinhard K Proels
Affiliation:
Chair of Phytopathology, Technische Universität München, Freising, Germany
For correspondence:
proels@wzw.tum.de
Bio-protocol author page:
a1779
,
date: 11/5/2014, 3757 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1286
[Abstract] Cyanobacteria are prokaryotes, which perform oxygenic photosynthesis. Among them, the unicellular cyanobacterium
Synechocystis
sp. PCC 6803 (hereafter
Synechocystis
) is a well characterized model system for studies on oxygenic photosynthesis, light signal ...
Article identifier: bio-protocol.org/e1286
Fractionation by Ultracentrifugation of Gram Negative Cytoplasmic and Membrane Proteins
Authors:
Sara M Sandrini
Sara M Sandrini
Affiliation:
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK
For correspondence:
sms26@le.ac.uk
Bio-protocol author page:
a1780
,
Richard Haigh
Richard Haigh
Affiliation:
Department of Genetics, University of Leicester, Leicester, UK
Bio-protocol author page:
a1781
and
Primrose P. E. Freestone
Primrose P. E. Freestone
Affiliation:
Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, UK
Bio-protocol author page:
a1782
,
date: 11/5/2014, 5870 views,
1 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1287
[Abstract] Protein fractionation is a useful separation process which divides membrane proteins (including those located in the outer and inner membrane) and cytoplasmic proteins into discrete fractions. Fractionation of proteins can simplify analysis of the numbers ...
Article identifier: bio-protocol.org/e1287
Neuroscience
An Experimental Model of Neonatal Nociceptive Stimulation in Rats
Authors:
Jackeline Moraes Malheiros
Jackeline Moraes Malheiros
Affiliation:
Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, Brazil
Bio-protocol author page:
a1765
,
Cristiane Amaral
Cristiane Amaral
Affiliation:
Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, Brazil
Bio-protocol author page:
a1766
,
Ana Teresa S Leslie
Ana Teresa S Leslie
Affiliation:
Departamento de Pediatria, Universidade Federal de São Paulo, São Paulo, Brazil
Bio-protocol author page:
a1767
,
Ruth Guinsburg
Ruth Guinsburg
Affiliation:
Departamento de Pediatria, Universidade Federal de São Paulo, São Paulo, Brazil
Bio-protocol author page:
a1768
and
Luciene Covolan
Luciene Covolan
Affiliation:
Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, Brazil
For correspondence:
lcovolan@unifesp.br
Bio-protocol author page:
a1769
,
date: 11/5/2014, 3423 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1283
[Abstract] In order to survive, preterm and/or sick neonates need diagnostic and therapeutic measures that may cause discomfort, stress and pain during a critical period of intense growing and modeling of the central nervous system (Anand
et al.
, 2013). Scientific ...
Article identifier: bio-protocol.org/e1283
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Pain Assessment Using the Rat and Mouse Formalin Tests
Authors:
Nian Gong
Nian Gong
Affiliation:
King’s Lab, Shanghai Jiao Tong University School of Pharmacy, Shanghai, China
Bio-protocol author page:
a1783
,
Qian Huang
Qian Huang
Affiliation:
King’s Lab, Shanghai Jiao Tong University School of Pharmacy, Shanghai, China
Bio-protocol author page:
a1784
,
Yuan Chen
Yuan Chen
Affiliation:
King’s Lab, Shanghai Jiao Tong University School of Pharmacy, Shanghai, China
Bio-protocol author page:
a1785
,
Meng Xu
Meng Xu
Affiliation:
King’s Lab, Shanghai Jiao Tong University School of Pharmacy, Shanghai, China
Bio-protocol author page:
a1786
,
Shuai Ma
Shuai Ma
Affiliation:
King’s Lab, Shanghai Jiao Tong University School of Pharmacy, Shanghai, China
Bio-protocol author page:
a1787
and
Yong-Xiang Wang
Yong-Xiang Wang
Affiliation:
King’s Lab, Shanghai Jiao Tong University School of Pharmacy, Shanghai, China
For correspondence:
yxwang@sjtu.edu.cn
Bio-protocol author page:
a1788
,
date: 11/5/2014, 7421 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1288
[Abstract] The formalin test was originally developed by Dubuisson and Dennis (1977), and has since been extensively used to assess pain-related responses. Rats and mice are the most frequently used animal models, though other species including cats, rabbits, guinea ...
Article identifier: bio-protocol.org/e1288
Plant Science
Preparation of Multiplexed Small RNA Libraries From Plants
Authors:
Kerrigan B. Gilbert
Kerrigan B. Gilbert
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, USA
Bio-protocol author page:
a1745
,
Noah Fahlgren
Noah Fahlgren
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, USA
Bio-protocol author page:
a1746
,
Kristin D. Kasschau
Kristin D. Kasschau
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, USA
Bio-protocol author page:
a1747
,
Elisabeth J. Chapman
Elisabeth J. Chapman
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, USA
Bio-protocol author page:
a1748
,
James C. Carrington
James C. Carrington
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, USA
Bio-protocol author page:
a1749
and
Alberto Carbonell
Alberto Carbonell
Affiliation:
Donald Danforth Plant Science Center, Saint Louis, USA
For correspondence:
acarbonell@ibmcp.upv.es
Bio-protocol author page:
a1750
,
date: 11/5/2014, 4851 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1275
[Abstract] High-throughput sequencing is a powerful tool for exploring small RNA populations in plants. The ever-increasing output from an Illumina Sequencing System allows for multiplexing multiple samples while still obtaining sufficient data for small RNA discovery ...
Article identifier: bio-protocol.org/e1275
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Extraction of Total Proteins from Rice Plant
Authors:
Da-Gin Lin
Da-Gin Lin
Affiliation:
Department of Agronomy, National Chung-Hsing University, Taichung, Taiwan
Bio-protocol author page:
a1757
and
Chang-Sheng Wang
Chang-Sheng Wang
Affiliation:
Department of Agronomy, National Chung-Hsing University, Taichung, Taiwan
For correspondence:
wangchansen@nchu.edu.tw
Bio-protocol author page:
a1758
,
date: 11/5/2014, 9241 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1277
[Abstract] This protocol provides an efficient method for preparation of high-quality proteins from rice leaves and grains. The method involves phenol extraction to separate proteins from the non-protein components such as polysaccharides, lipids and phenolic compounds ...
Article identifier: bio-protocol.org/e1277
Measurement of the Number of Peroxisomes
Authors:
Michitaro Shibata
Michitaro Shibata
Affiliation:
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan
Bio-protocol author page:
a1770
,
Kazusato Oikawa
Kazusato Oikawa
Affiliation:
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan
Bio-protocol author page:
a1771
,
Shoji Mano
Shoji Mano
Affiliation:
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan
Bio-protocol author page:
a1772
and
Mikio Nishimura
Mikio Nishimura
Affiliation:
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan
For correspondence:
mikion@nibb.ac.jp
Bio-protocol author page:
a1773
,
date: 11/5/2014, 2834 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1284
[Abstract] This is the detailed protocol for the measurement of the number of peroxisomes described by Shibata
et al.
(2013). It is difficult to count the number of organelles in a cell because of the thickness of plant leaves. To overcome this challenge, protoplasts ...
Article identifier: bio-protocol.org/e1284
In vitro
Assay of the Glycosyltransferase Activity of a Heterologously Expressed Plant Protein
Authors:
Carsten P Ade
Carsten P Ade
Affiliation 1:
Scion, Rotorua, New Zealand
Affiliation 2:
School of Biological Sciences, The University of Auckland, Auckland, New Zealand
Present address:
Department of Biochemistry and Molecular Biology, University of Wuerzburg, Wuerzburg, Germany
Bio-protocol author page:
a1774
,
Felix Bemm
Felix Bemm
Affiliation:
Department of Bioinformatics, University of Wuerzburg, Wuerzburg, Germany
Bio-protocol author page:
a1775
,
James M J Dickson
James M J Dickson
Affiliation:
School of Biological Sciences, The University of Auckland, Auckland, New Zealand
Bio-protocol author page:
a1776
,
Christian Walter
Christian Walter
Affiliation:
Scion, Rotorua, New Zealand
Bio-protocol author page:
a1777
and
Philip J Harris
Philip J Harris
Affiliation:
School of Biological Sciences, The University of Auckland, Auckland, New Zealand
For correspondence:
p.harris@auckland.ac.nz
Bio-protocol author page:
a1778
,
date: 11/5/2014, 3096 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1285
[Abstract] Glycosyltransferases are carbohydrate active enzymes containing catalytic modules involved in catalysing the biosynthesis of glycosidic bonds in oligo- and polysaccharides and glycoconjugates. One of the most comprehensive collections of Carbohydrate ...
Article identifier: bio-protocol.org/e1285
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