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Volume:4 Issue:14
July 20, 2014
Cancer Biology
Bone Resorption Assay
Authors:
Carina Scholtysek
Carina Scholtysek
Affiliation:
Department of Internal Medicine 3, University of Erlangen-Nuremberg, Institute of Rheumatology and Immunology, Erlangen, Germany
For correspondence:
carina.scholtysek@uk-erlangen.de
Bio-protocol author page:
a1500
,
Gerhard Krönke
Gerhard Krönke
Affiliation:
Department of Internal Medicine 3, University of Erlangen-Nuremberg, Institute of Rheumatology and Immunology, Erlangen, Germany
For correspondence:
gerhard.kroenke@uk-erlangen.de
Bio-protocol author page:
a290
and
Georg Schett
Georg Schett
Affiliation:
Department of Internal Medicine 3, University of Erlangen-Nuremberg, Institute of Rheumatology and Immunology, Erlangen, Germany
For correspondence:
georg.schett@uk-erlangen.de
Bio-protocol author page:
a1502
,
date: 7/20/2014, 4647 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1187
[Abstract] The Bone resorption assay provides an easy to use protocol for quantitatively measuring
in vitro
osteoclast-mediated bone resorption. Osteoclasts can be seeded onto the bone slices and formation of resorption pits can be quantified via toluidinblue staining ...
Article identifier: bio-protocol.org/e1187
Developmental Biology
Hedgehog (Hh) Reporter Activity Assay
Author:
Chen Zhao
Chen Zhao
Affiliation:
Department of Developmental Biology, Institute for Stem Cell and Regenerative Medicine, Stanford University, Stanford, USA
For correspondence:
chenzhao@stanford.edu
Bio-protocol author page:
a1513
,
date: 7/20/2014, 4700 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1182
[Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Hh ligands or Hh pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase ...
Article identifier: bio-protocol.org/e1182
Wnt Reporter Activity Assay
Author:
Chen Zhao
Chen Zhao
Affiliation:
Department of Developmental Biology, Institute for Stem Cell and Regenerative Medicine, Stanford University, Stanford, USA
For correspondence:
chenzhao@stanford.edu
Bio-protocol author page:
a1513
,
date: 7/20/2014, 8477 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1183
[Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Wnt ligands or Wnt pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase ...
Article identifier: bio-protocol.org/e1183
Fluorescence Microscopy for Cilia in Cultured Cells and Zebrafish Embryos
Authors:
Jingli Cao
Jingli Cao
Affiliation:
State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai, China
Bio-protocol author page:
a1497
,
Xueliang Zhu
Xueliang Zhu
Affiliation:
State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai, China
Bio-protocol author page:
a1498
and
Xiumin Yan
Xiumin Yan
Affiliation:
State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai, China
For correspondence:
yanx@sibcb.ac.cn
Bio-protocol author page:
a1499
,
date: 7/20/2014, 5113 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1188
[Abstract] Cilia are microtubule-based hair-like projections found in organisms, ranging from protozoa to mammals. This protocol provides methods for immunofluorescence staining of cilia in cultured cells and zebrafish embryos....
Article identifier: bio-protocol.org/e1188
Immunology
Isolation of ILC2 from Mouse Liver
Authors:
Tamar Mchedlidze
Tamar Mchedlidze
Affiliation:
Medical Department 1, FAU Erlangen-Nuremberg, Erlangen, Germany
Bio-protocol author page:
a1516
and
Stefan Wirtz
Stefan Wirtz
Affiliation:
Medical Department 1, FAU Erlangen-Nuremberg, Erlangen, Germany
For correspondence:
stefan.wirtz@uk-erlangen.de
Bio-protocol author page:
a1517
,
date: 7/20/2014, 3695 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1179
[Abstract] Group 2 innate lymphoid cells (ILC2) are a recently characterized cell population which lacks specific antigen receptors and contributes to immune responses at mucosal surfaces of lung and gut. Recently, we demonstrated that ILC2 expand in the context ...
Article identifier: bio-protocol.org/e1179
Macrophage Inflammatory Assay
Authors:
Thomas J. Bartosh
Thomas J. Bartosh
Affiliation:
Institute for Regenerative Medicine, Texas A&M University Health Science Center, Temple, USA
For correspondence:
bartosh@medicine.tamhsc.edu
Bio-protocol author page:
a1514
and
Joni H. Ylostalo
Joni H. Ylostalo
Affiliation:
Institute for Regenerative Medicine, Texas A&M University Health Science Center, Temple, USA
Bio-protocol author page:
a1515
,
date: 7/20/2014, 10537 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1180
[Abstract] Macrophages represent a widely distributed and functionally diverse population of innate myeloid cells involved in inflammatory response to pathogens, tissue homeostasis and tissue repair (Murray and Wynn, 2011). Macrophages can be broadly grouped into ...
Article identifier: bio-protocol.org/e1180
Microbiology
In vitro
EBV Infection of Mononuclear Cells that Have Been Cryo-preserved
Authors:
Shanie Saghafian-Hedengren
Shanie Saghafian-Hedengren
Affiliation:
Department of Molecular Biosciences, Stockholm University/Wenner-Gren Institute, Stockholm, Sweden
For correspondence:
shanie.hedengren@ki.se
Bio-protocol author page:
a1523
,
Ebba Sohlberg
Ebba Sohlberg
Affiliation:
Department of Molecular Biosciences, Stockholm University/Wenner-Gren Institute, Stockholm, Sweden
Bio-protocol author page:
a1524
,
Jakob Theorell
Jakob Theorell
Affiliation:
Department of Medicine , Center for Infectious Medicine/Karolinska Institute, Stockholm, Sweden
Bio-protocol author page:
a1525
,
Claudia Carvalho-Queiroz
Claudia Carvalho-Queiroz
Affiliation:
Department of Molecular Biosciences, Stockholm University/The Wenner-Gren Institute, Stockholm, Sweden
Bio-protocol author page:
a1526
,
Noémi Nagy
Noémi Nagy
Affiliation:
Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
Bio-protocol author page:
a1527
,
Jan-Olov Persson
Jan-Olov Persson
Affiliation:
Department of Mathematics, Stockholm University, Stockholm, Sweden
Bio-protocol author page:
a1528
,
Caroline Nilsson
Caroline Nilsson
Affiliation:
Department of Clinical Science and Education, Karolinska Institutet and Sachs' Children's Hospital, Stockholm, Sweden
Bio-protocol author page:
a1529
,
Yenan T. Bryceson
Yenan T. Bryceson
Affiliation:
Department of Medicine, Center for Infectious Medicine/Karolinska Institute, Stockholm, Sweden
Bio-protocol author page:
a1530
and
Eva Sverremark-Ekström
Eva Sverremark-Ekström
Affiliation:
Department of Molecular Biosciences, Stockholm University/Wenner-Gren Institute, Stockholm, Sweden
Bio-protocol author page:
a1531
,
date: 7/20/2014, 2243 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1177
[Abstract] Epstein-Barr Virus (EBV) is a B-lymphotropic herpesvirus which the majority of adult human population is latently-infected with. Various immunological and molecular
in vitro
studies have been facilitated by the use of EBV’s ability to infect and transform ...
Article identifier: bio-protocol.org/e1177
Affinofile Assay for Identifying Macrophage-Tropic HIV-1
Authors:
Sarah B. Joseph
Sarah B. Joseph
Affiliation:
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, USA
For correspondence:
sbjoseph@email.unc.edu
Bio-protocol author page:
a1510
,
Benhur Lee
Benhur Lee
Affiliation:
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, USA
Bio-protocol author page:
a1511
and
Ronald Swanstrom
Ronald Swanstrom
Affiliation 1:
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, USA
Affiliation 2:
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, USA
For correspondence:
risunc@med.unc.edu
Bio-protocol author page:
a1512
,
date: 7/20/2014, 2502 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1184
[Abstract] The ability to enter monocyte-derived macrophage (MDM)
in vitro
is commonly used to define macrophage-tropic HIV-1 despite the fact that viruses vary continuously in their ability to enter MDMs
in vitro
, and MDMs vary in their ability to support HIV-1 ...
Article identifier: bio-protocol.org/e1184
Chromatin Fractionation Assay in Fission Yeast
Authors:
Tatsuki Kunoh
Tatsuki Kunoh
Affiliation:
Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan
For correspondence:
tkunoh06@gmail.com
Bio-protocol author page:
a1508
and
Toshiyuki Habu
Toshiyuki Habu
Affiliation:
Radiation Biology Center, Kyoto University, Kyoto, Japan
Bio-protocol author page:
a1509
,
date: 7/20/2014, 3136 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1185
[Abstract] The protein recruitment onto chromatin is a critical process for DNA metabolism, including DNA replication, DNA repair and DNA recombination. Especially DNA modification enzymes and checkpoint proteins are loaded onto DNA damage sites in a context-dependent ...
Article identifier: bio-protocol.org/e1185
Development of a Novel Assay for Synthesis and Hydrolysis of Sedoheptulose 1,7-bisphosphate (SBP)
in vitro
by Combinations of Purified Fructose 1,6-bisphosphate aldolases (FBA) Proteins and Fructose 1,6-bisphosphatases (FBPase) Proteins from
Bacillus methanolicus
MGA3
Authors:
Jessica Stolzenberger
Jessica Stolzenberger
Affiliation:
Genetics of Prokaryotes, Bielefeld University, Bielefeld, Germany
Bio-protocol author page:
a1503
,
Steffen N. Lindner
Steffen N. Lindner
Affiliation:
Genetics of Prokaryotes, Bielefeld University, Bielefeld, Germany
Bio-protocol author page:
a1504
,
Marcus Persicke
Marcus Persicke
Affiliation:
Institute for Genome Research and Systems Biology, Bielefeld University, Bielefeld, Germany
Bio-protocol author page:
a1505
,
Trygve Brautaset
Trygve Brautaset
Affiliation:
Biotechnology and Nanomedicine, SINTEF Materials and Chemistry, Trondheim, Norway
Bio-protocol author page:
a1506
and
Volker F. Wendisch
Volker F. Wendisch
Affiliation:
Genetics of Prokaryotes, Bielefeld University, Bielefeld, Germany
For correspondence:
volker.wendisch@uni-bielefeld.de
Bio-protocol author page:
a1507
,
date: 7/20/2014, 2720 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1186
[Abstract]
Bacillus methanolicus
(
B. methanolicus
) is a Gram-positive, thermotolerant, and facultative methylotrophic bacterium that can use the one-carbon (C1) compound methanol as a source of carbon and energy (Schendel
et al.
, 1990; Arfman
et al.
, 1997; Arfman ...
Article identifier: bio-protocol.org/e1186
Dye Release Experiments with Dextran Loaded Vesicles
Authors:
Marc-Antoine Sani
Marc-Antoine Sani
Affiliation:
School of Chemistry, The University of Melbourne, Melbourne, Australia
For correspondence:
msani@unimelb.edu.au
Bio-protocol author page:
a1492
,
Neil M. O’Brien-Simpson
Neil M. O’Brien-Simpson
Affiliation:
Melbourne Dental School, The University of Melbourne, Melbourne, Australia
Bio-protocol author page:
a1488
and
Frances Separovic
Frances Separovic
Affiliation:
School of Chemistry, The University of Melbourne, Melbourne, Australia
Bio-protocol author page:
a1493
,
date: 7/20/2014, 2741 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1190
[Abstract] Dye release experiments are a widely used method to assess the interactions between membrane-active molecules and lipid membranes. Of particular interest is the ability to assess the degree of the lipid bilayer perturbation by simultaneously encapsulating ...
Article identifier: bio-protocol.org/e1190
Bacterial Fluorescent-dextran Diffusion Assay
Authors:
Neil M. O’Brien-Simpson
Neil M. O’Brien-Simpson
Affiliation:
Melbourne Dental School, The University of Melbourne, Melbourne, Australia
For correspondence:
neil.obs@unimelb.edu.au
Bio-protocol author page:
a1488
,
Namfon Pantarat
Namfon Pantarat
Affiliation:
Melbourne Dental School, The University of Melbourne, Melbourne, Australia
Bio-protocol author page:
a1489
,
Katrina A. Walsh
Katrina A. Walsh
Affiliation:
Melbourne Dental School, The University of Melbourne, Melbourne, Australia
Bio-protocol author page:
a1490
,
Eric C. Reynolds
Eric C. Reynolds
Affiliation:
Melbourne Dental School, The University of Melbourne, Melbourne, Australia
Bio-protocol author page:
a1491
,
Marc-Antoine Sani
Marc-Antoine Sani
Affiliation:
School of Chemistry, University of Melbourne, Melbourne, Australia
Bio-protocol author page:
a1492
and
Frances Separovic
Frances Separovic
Affiliation:
School of Chemistry, University of Melbourne, Melbourne, Australia
Bio-protocol author page:
a1493
,
date: 7/20/2014, 2655 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1191
[Abstract] Antimicrobial peptides are known to disrupt bacterial membranes allowing solutes to flow across the membrane in an unregulated manner resulting in death of the organism. Disrupting the bacterial membrane would thus perturb the cells osmotic balance resulting ...
Article identifier: bio-protocol.org/e1191
Plant Science
Rice Meiotic Chromosome Spread Preparation of Pollen Mother Cells
Authors:
Xingwang Li
Xingwang Li
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
Bio-protocol author page:
a1495
and
Changyin Wu
Changyin Wu
Affiliation:
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China
For correspondence:
cywu@mail.hzau.edu.cn
Bio-protocol author page:
a1496
,
date: 7/20/2014, 2869 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1189
[Abstract] In this protocol, we describe a simple and efficient method for meiotic chromosome spread preparation in rice pollen mother cells. Meiotic chromosome preparation by spreading itself is an important technique for plant cytogenetics (Higgins
et al.
, 2004; ...
Article identifier: bio-protocol.org/e1189
Stem Cell
Coculture between hMADS and Mouse Adult CM
Authors:
Florence Figeac
Florence Figeac
Affiliation:
INSERM U955, INSERM, Créteil , France
Bio-protocol author page:
a1518
,
Adrien Acquistapace
Adrien Acquistapace
Affiliation:
INSERM U955, INSERM, Créteil, France
Bio-protocol author page:
a1519
,
Olivier Le Coz
Olivier Le Coz
Affiliation:
INSERM U955, INSERM, Créteil, France
Bio-protocol author page:
a1520
,
Pierre François Lesault
Pierre François Lesault
Affiliation:
INSERM U955, INSERM, Créteil, France
Bio-protocol author page:
a1521
and
Anne-Marie Rodriguez
Anne-Marie Rodriguez
Affiliation:
INSERM U955, INSERM, Créteil, France
For correspondence:
anne-marie.rodriguez@inserm.fr
Bio-protocol author page:
a1522
,
date: 7/20/2014, 3345 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1178
[Abstract] Heart failure occurring after acute myocardial infarction (MI) is among the main causes of death in western countries. Cell therapies, particularly those based on mesenchymal stem cells (MSC), represent one of the most promising approaches to repair ...
Article identifier: bio-protocol.org/e1178
Mesenchymal Stem Cell (MSC) Aggregate Formation
in vivo
Authors:
Thomas J. Bartosh
Thomas J. Bartosh
Affiliation:
Institute for Regenerative Medicine, Texas A&M University Health Science Center, Temple, USA
For correspondence:
bartosh@medicine.tamhsc.edu
Bio-protocol author page:
a1514
and
Joni H. Ylostalo
Joni H. Ylostalo
Affiliation:
Institute for Regenerative Medicine, Texas A&M University Health Science Center, Temple, USA
Bio-protocol author page:
a1515
,
date: 7/20/2014, 3992 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1181
[Abstract] Human mesenchymal stem/progenitor cells (MSCs) isolated from various adult tissues show remarkable therapeutic potential and are being employed in clinical trials for the treatment of numerous diseases (Prockop
et al.
, 2010). While routes of cell administration ...
Article identifier: bio-protocol.org/e1181
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