Cancer Biology

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model

Authors: Ruth A. Franklin
Ruth A. FranklinAffiliation 1: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
Affiliation 2: Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, USA
Present address: , Yale University School of Medicine, New Haven, USA
For correspondence: ruth.franklin@yale.edu
Bio-protocol author page: a2488
and Ming O. Li
Ming O. LiAffiliation: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
For correspondence: lim@mskcc.org
Bio-protocol author page: a1463
, date: 8/20/2015, 2435 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1567.

[Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore potential candidates for therapeutic targeting ...

Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)

Authors: Damya Laoui
Damya LaouiAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1803
, Eva Van Overmeire
Eva Van OvermeireAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1804
, Jiri Keirsse
Jiri KeirsseAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1805
, Kiavash Movahedi
Kiavash MovahediAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1806
and Jo A Van Ginderachter
Jo A Van GinderachterAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
For correspondence: jvangind@vub.ac.be
Bio-protocol author page: a1807
, date: 11/20/2014, 7734 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1294.

[Abstract] Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored by clinical studies showing a correlation ...

Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)

Authors: Damya Laoui
Damya LaouiAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1803
, Eva Van Overmeire
Eva Van OvermeireAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1804
, Jiri Keirsse
Jiri KeirsseAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1805
, Kiavash Movahedi
Kiavash MovahediAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1806
and Jo A Van Ginderachter
Jo A Van GinderachterAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
For correspondence: jvangind@vub.ac.be
Bio-protocol author page: a1807
, date: 11/20/2014, 7734 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1294.

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model

Authors: Ruth A. Franklin
Ruth A. FranklinAffiliation 1: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
Affiliation 2: Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, USA
Present address: , Yale University School of Medicine, New Haven, USA
For correspondence: ruth.franklin@yale.edu
Bio-protocol author page: a2488
and Ming O. Li
Ming O. LiAffiliation: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
For correspondence: lim@mskcc.org
Bio-protocol author page: a1463
, date: 8/20/2015, 2435 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1567.

Cancer Biology

Angiogenesis

Cancer biochemistry

Cancer stem cell

Cell cycle checkpoints

Cell death

Cellular energetics

General technique

Genome instability & mutation

Inflammation

Invasion & metastasis

Proliferative signaling

Replicative immortality

Tumor immunology

Animal models

Cell isolation and culture

Cell transfer therapy

Drug discovery and analysis

Genetics

Immunological assays

Tumor formation

Tumor microenvironment

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