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Cancer Biology

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model

Authors: Ruth A. Franklin
Ruth A. FranklinAffiliation 1: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
Affiliation 2: Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, USA
Present address: , Yale University School of Medicine, New Haven, USA
For correspondence: ruth.franklin@yale.edu
Bio-protocol author page: a2488
 and Ming O. Li
Ming O. LiAffiliation: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
For correspondence: lim@mskcc.org
Bio-protocol author page: a1463
,  date: 8/20/2015, 2435 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1567.

[Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore potential candidates for therapeutic targeting ...

Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model

Authors: Libo Yan
Libo YanAffiliation: The institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China
Bio-protocol author page: a2461
 and Yan Xu
Yan XuAffiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, USA
For correspondence: xu2@iupui.edu
Bio-protocol author page: a2462
,  date: 8/20/2015, 1884 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1557.

[Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when (subcutaneously) ...

Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)

Authors: Damya Laoui
Damya LaouiAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1803
, Eva Van Overmeire
Eva Van OvermeireAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1804
, Jiri Keirsse
Jiri KeirsseAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1805
, Kiavash Movahedi
Kiavash MovahediAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1806
 and Jo A Van Ginderachter
Jo A Van GinderachterAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
For correspondence: jvangind@vub.ac.be
Bio-protocol author page: a1807
,  date: 11/20/2014, 7733 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1294.

[Abstract] Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored by clinical studies showing a correlation ...

Natural Killer Cell Transfer Assay

Authors: Lee-Hwa Tai
Lee-Hwa TaiAffiliation: Centre for Innovative Cancer Research, Ottawa Hospital Research Institute, Ottawa, Canada
Bio-protocol author page: a764
, Christiano Tanese de Souza
Christiano Tanese de SouzaAffiliation: Centre for Innovative Cancer Research, Ottawa Hospital Research Institute, Ottawa, Canada
Bio-protocol author page: a765
, Andrew P. Makrigiannis
Andrew P. MakrigiannisAffiliation: Biochemistry, Microbiology and Immunology Department, University of Ottawa, Ottawa, Canada
Bio-protocol author page: a766
 and Rebecca Ann C. Auer
Rebecca Ann C. AuerAffiliation: Department of Surgery, University of Ottawa, Ottawa, Canada
For correspondence: rauer@ottawahospital.on.ca
Bio-protocol author page: a767
,  date: 8/20/2013, 3877 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.864.

[Abstract] Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol will describe the adoptive transfer of purified ...

Lung Clearance Assay

Authors: Pinchas Tsukerman
Pinchas TsukermanAffiliation: Lautenberg Center for General and Tumor Immunology, Hebrew University, Jerusalem, Israel
Bio-protocol author page: a244
 and Ofer Mandelboim
Ofer MandelboimAffiliation: Lautenberg Center for General and Tumor Immunology, Hebrew University, Jerusalem, Israel
For correspondence: oferm@ekmd.huji.ac.il
Bio-protocol author page: a245
,  date: 3/5/2013, 3933 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.336.

[Abstract] Lung clearance assay tests the ability of innate immune cells (mainly NK cells) to eradicate in vivo cells injected via the tail vein of the mice. This assay helps to elucidate the role played by NK cells and their receptors (if the mice are genetically modified) against various human and mouse targets in an in vivo setting (Stern-Ginossar et al., ...
1 

Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)

Authors: Damya Laoui
Damya LaouiAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1803
, Eva Van Overmeire
Eva Van OvermeireAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1804
, Jiri Keirsse
Jiri KeirsseAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1805
, Kiavash Movahedi
Kiavash MovahediAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
Bio-protocol author page: a1806
 and Jo A Van Ginderachter
Jo A Van GinderachterAffiliation 1: Myeloid Cell Immunology Laboratory, Brussels, Belgium
Affiliation 2: Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium
For correspondence: jvangind@vub.ac.be
Bio-protocol author page: a1807
,  date: 11/20/2014, 7734 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1294.

[Abstract] Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored ...

Lung Clearance Assay

Authors: Pinchas Tsukerman
Pinchas TsukermanAffiliation: Lautenberg Center for General and Tumor Immunology, Hebrew University, Jerusalem, Israel
Bio-protocol author page: a244
 and Ofer Mandelboim
Ofer MandelboimAffiliation: Lautenberg Center for General and Tumor Immunology, Hebrew University, Jerusalem, Israel
For correspondence: oferm@ekmd.huji.ac.il
Bio-protocol author page: a245
,  date: 3/5/2013, 3933 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.336.

[Abstract] Lung clearance assay tests the ability of innate immune cells (mainly NK cells) to eradicate in vivo cells injected via the tail vein of the mice. This assay helps to elucidate the role played by NK cells and their receptors (if the mice are genetically modified) against various human and mouse targets ...

Natural Killer Cell Transfer Assay

Authors: Lee-Hwa Tai
Lee-Hwa TaiAffiliation: Centre for Innovative Cancer Research, Ottawa Hospital Research Institute, Ottawa, Canada
Bio-protocol author page: a764
, Christiano Tanese de Souza
Christiano Tanese de SouzaAffiliation: Centre for Innovative Cancer Research, Ottawa Hospital Research Institute, Ottawa, Canada
Bio-protocol author page: a765
, Andrew P. Makrigiannis
Andrew P. MakrigiannisAffiliation: Biochemistry, Microbiology and Immunology Department, University of Ottawa, Ottawa, Canada
Bio-protocol author page: a766
 and Rebecca Ann C. Auer
Rebecca Ann C. AuerAffiliation: Department of Surgery, University of Ottawa, Ottawa, Canada
For correspondence: rauer@ottawahospital.on.ca
Bio-protocol author page: a767
,  date: 8/20/2013, 3877 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.864.

[Abstract] Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol ...

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model

Authors: Ruth A. Franklin
Ruth A. FranklinAffiliation 1: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
Affiliation 2: Graduate Program in Immunology and Microbial Pathogenesis, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, USA
Present address: , Yale University School of Medicine, New Haven, USA
For correspondence: ruth.franklin@yale.edu
Bio-protocol author page: a2488
 and Ming O. Li
Ming O. LiAffiliation: Immunology Program, Memorial Sloan Kettering Cancer Center, New York, USA
For correspondence: lim@mskcc.org
Bio-protocol author page: a1463
,  date: 8/20/2015, 2435 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1567.

[Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore ...

Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model

Authors: Libo Yan
Libo YanAffiliation: The institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China
Bio-protocol author page: a2461
 and Yan Xu
Yan XuAffiliation: Department of Obstetrics and Gynecology, Indiana University School of Medicine, Indianapolis, USA
For correspondence: xu2@iupui.edu
Bio-protocol author page: a2462
,  date: 8/20/2015, 1884 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1557.

[Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP ...
1 
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