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Permanent Occlusion of the Left Anterior Coronary Artery in the Rat
Authors:
Mélanie Bousquenaud
Mélanie Bousquenaud
Affiliation:
Laboratory of Pathology, Dept of Medicine, University of Fribourg, Fribourg, Switzerland
For correspondence:
melanie.bousquenaud@unifr.ch
Bio-protocol author page:
a2737
,
Pierre-Yves Marie
Pierre-Yves Marie
Affiliation:
Nancyclotep, Experimental Imaging Platform, Nancy, France
Bio-protocol author page:
a2738
and
Yvan Devaux
Yvan Devaux
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institut of Health, Luxembourg, Luxembourg
Bio-protocol author page:
a2642
,
date: 11/20/2015, 1366 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1663
.
[Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological process is the main cause of cardiac evolution toward ...
Rat Aortic Ring Model to Assay Angiogenesis
ex vivo
Authors:
Isabelle Ernens
Isabelle Ernens
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
For correspondence:
isabelle.ernens@lih.lu
Bio-protocol author page:
a2640
,
Bénédicte Lenoir
Bénédicte Lenoir
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page:
a2641
,
Yvan Devaux
Yvan Devaux
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page:
a2642
and
Daniel R. Wagner
Daniel R. Wagner
Affiliation:
Division of Cardiology, Centre Hospitalier, Luxembourg, Luxembourg
Bio-protocol author page:
a2643
,
date: 10/20/2015, 2151 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1622
.
[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using
in vitro
assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of endothelial cells ...
Gastric Aspiration Models
Authors:
Bruce A. Davidson
Bruce A. Davidson
Affiliation:
Department of Anesthesiology, and Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, USA
For correspondence:
bdavidso@buffalo.edu
Bio-protocol author page:
a1019
and
Ravi Alluri
Ravi Alluri
Affiliation:
Department of Anesthesiology, and Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, USA
Bio-protocol author page:
a1020
,
date: 11/20/2013, 7440 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.968
.
[Abstract] The procedures described below are for producing gastric aspiration pneumonitis in mice with alterations for rats and rabbits described parenthetically. We use 4 different injury vehicles delivered intratracheally to investigate the inflammatory responses to gastric aspiration:
1) Normal saline (NS) as the injury vehicle control
2) NS + HCl, pH = 1.25 ...
Evaluation of Caspase-1 Activation and IL-1β Production in A Kainic Acid Microdyalisis Brain Injury Model
Authors:
Antonio S. Herranz
Antonio S. Herranz
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
For correspondence:
antonio.sanchez@hrc.es
Bio-protocol author page:
a583
,
Eulalia Bazán
Eulalia Bazán
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a584
,
Diana Reimers
Diana Reimers
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a585
,
María T. Montero-Vega
María T. Montero-Vega
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a586
,
Adriano Jménez-Escrig
Adriano Jménez-Escrig
Affiliation:
Medicina y Especialidades Médicas, Universidad de Alcalá, Madrid, Spain
Bio-protocol author page:
a587
and
Pablo Pelegrín
Pablo Pelegrín
Affiliation:
Inflammation and Experimental Surgery Unit, University Clinical Hospital Virgen Arrixaca, Murcia, Spain
For correspondence:
pablo.pelegrin@ffis.es
Bio-protocol author page:
a326
,
date: 4/20/2013, 3672 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.433
.
[Abstract] Intracerebral infusion of kainic acid (KA) by a microdialysis probe induces a focal swelling in the brain-perfused area which promotes inflammation (Compan
et al
., 2012; Oprica
et al
., 2003). The microdialysis technique allows the local
in vivo
perfusion of KA and the simultaneous collection of inflammatory mediators, and other neuroactive substances, ...
1
Gastric Aspiration Models
Authors:
Bruce A. Davidson
Bruce A. Davidson
Affiliation:
Department of Anesthesiology, and Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, USA
For correspondence:
bdavidso@buffalo.edu
Bio-protocol author page:
a1019
and
Ravi Alluri
Ravi Alluri
Affiliation:
Department of Anesthesiology, and Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, USA
Bio-protocol author page:
a1020
,
date: 11/20/2013, 7440 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.968
.
[Abstract] The procedures described below are for producing gastric aspiration pneumonitis in mice with alterations for rats and rabbits described parenthetically. We use 4 different injury vehicles delivered intratracheally to investigate the inflammatory responses to gastric aspiration:
1) Normal saline (NS) ...
Evaluation of Caspase-1 Activation and IL-1β Production in A Kainic Acid Microdyalisis Brain Injury Model
Authors:
Antonio S. Herranz
Antonio S. Herranz
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
For correspondence:
antonio.sanchez@hrc.es
Bio-protocol author page:
a583
,
Eulalia Bazán
Eulalia Bazán
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a584
,
Diana Reimers
Diana Reimers
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a585
,
María T. Montero-Vega
María T. Montero-Vega
Affiliation:
Neurobiology Service, Hospital Ramón y Cajal, Madrid, Spain
Bio-protocol author page:
a586
,
Adriano Jménez-Escrig
Adriano Jménez-Escrig
Affiliation:
Medicina y Especialidades Médicas, Universidad de Alcalá, Madrid, Spain
Bio-protocol author page:
a587
and
Pablo Pelegrín
Pablo Pelegrín
Affiliation:
Inflammation and Experimental Surgery Unit, University Clinical Hospital Virgen Arrixaca, Murcia, Spain
For correspondence:
pablo.pelegrin@ffis.es
Bio-protocol author page:
a326
,
date: 4/20/2013, 3672 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.433
.
[Abstract] Intracerebral infusion of kainic acid (KA) by a microdialysis probe induces a focal swelling in the brain-perfused area which promotes inflammation (Compan
et al
., 2012; Oprica
et al
., 2003). The microdialysis technique allows the local
in vivo
perfusion of KA and the simultaneous collection of inflammatory ...
Rat Aortic Ring Model to Assay Angiogenesis
ex vivo
Authors:
Isabelle Ernens
Isabelle Ernens
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
For correspondence:
isabelle.ernens@lih.lu
Bio-protocol author page:
a2640
,
Bénédicte Lenoir
Bénédicte Lenoir
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page:
a2641
,
Yvan Devaux
Yvan Devaux
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institute of Health (L.I.H.), Luxembourg, Luxembourg
Bio-protocol author page:
a2642
and
Daniel R. Wagner
Daniel R. Wagner
Affiliation:
Division of Cardiology, Centre Hospitalier, Luxembourg, Luxembourg
Bio-protocol author page:
a2643
,
date: 10/20/2015, 2151 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1622
.
[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using
in vitro
assays such as Boyden chamber assay, wound healing assay and tube formation assay. These ...
Permanent Occlusion of the Left Anterior Coronary Artery in the Rat
Authors:
Mélanie Bousquenaud
Mélanie Bousquenaud
Affiliation:
Laboratory of Pathology, Dept of Medicine, University of Fribourg, Fribourg, Switzerland
For correspondence:
melanie.bousquenaud@unifr.ch
Bio-protocol author page:
a2737
,
Pierre-Yves Marie
Pierre-Yves Marie
Affiliation:
Nancyclotep, Experimental Imaging Platform, Nancy, France
Bio-protocol author page:
a2738
and
Yvan Devaux
Yvan Devaux
Affiliation:
Laboratory of Cardiovascular Research, Luxembourg Institut of Health, Luxembourg, Luxembourg
Bio-protocol author page:
a2642
,
date: 11/20/2015, 1366 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1663
.
[Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological ...
1
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