Welcome guest, Sign in

Home

Immunology

Qualitative and Quantitative Assay for Detection of Circulating Autoantibodies against Human Aortic Antigen

Authors: Brent Veerman
Brent VeermanAffiliation: Department of Surgery, University of Toledo College of Medicine and Life Sciences, Toledo, USA
Bio-protocol author page: a4752
 and Ritu Chakravarti
Ritu ChakravartiAffiliation: Department of Surgery, University of Toledo College of Medicine and Life Sciences, Toledo, USA
For correspondence: ritu.chakravarti@utoledo.edu
Bio-protocol author page: a3737
,  date: 7/5/2017, 135 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2367.

[Abstract] Increased amount of autoantibodies in human sera are the hallmark of autoimmune diseases (Wang et al., 2015). In case of known antigen, detection of autoantibodies is done using laboratory based methods. However, in most autoimmune diseases, knowledge of self-antigen is still vague. We have developed an ELISA-based quantitative assay to detect the ...

Coupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays

Authors: Jidnyasa Ingale
Jidnyasa IngaleAffiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, USA
Bio-protocol author page: a2626
 and Richard T Wyatt
Richard T WyattAffiliation 1: Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, USA
Affiliation 2: , IAVI Neutralizing Antibody Center at TSRI, La Jolla, USA
For correspondence: wyatt@scripps.edu
Bio-protocol author page: a2627
,  date: 10/5/2015, 1536 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1614.

[Abstract] Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process. Fine mapping of epitope specific antibodies is difficult when they are elicited at relatively low levels. In our recent study focused at developing an HIV-1 vaccine, we immunized rabbits ...

Pulse Chase of Suspension Cells

Authors: Lai-Yee Wong
Lai-Yee WongAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
For correspondence: laiyee.wong@usc.edu
Bio-protocol author page: a1453
, QiMing Liang
QiMing LiangAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
Bio-protocol author page: a1454
, Kevin Brulois
Kevin BruloisAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
Bio-protocol author page: a1455
 and Jae Jung
Jae JungAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
Bio-protocol author page: a1456
,  date: 7/5/2014, 3746 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1170.

[Abstract] Pulse-chase method is a powerful technique used to follow the dynamics of proteins over a period of time. The expression level, processing, transport, secretion or half-life of proteins can be tracked by metabolically labeling the cells, such as with radiolabeled amino acids (pulse step). This protocol describes the condition used to study the folding ...

Monoclonal Antibody Purification (Nicotiana benthamiana Plants)

Authors: Adam Husk
Adam HuskAffiliation: Owensboro Cancer Research Program at James Graham Brown Cancer Center, University of Louisville School of Medicine, Owensboro, USA
Bio-protocol author page: a1126
, Krystal Teasley Hamorsky
Krystal Teasley Hamorsky Affiliation: Owensboro Cancer Research Program at James Graham Brown Cancer Center, University of Louisville School of Medicine, Owensboro, USA
Bio-protocol author page: a1127
 and Nobuyuki Matoba
Nobuyuki MatobaAffiliation: Department of Pharmacology and Toxicology, and Owensboro Cancer Research Program at James Graham Brown Cancer Center, University of Louisville School of Medicine, Owensboro, USA
For correspondence: n.matoba@louisville.edu
Bio-protocol author page: a1128
,  date: 1/20/2014, 4998 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1034.

[Abstract] Plant-based expression systems provide an alternative biomanufacturing platform for recombinant proteins (Matoba et al., 2011). In particular, plant virus-based vectors can overexpress proteins within days in the leaf tissue of Nicotiana benthamiana (N. benthamiana). To overcome the issues of genetic instability and limited infectivity of recombinant ...

[Bio101] Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies

Author: Zheng Liu
Zheng LiuAffiliation: The Feinstein Institute for Medical Research, Manhasset, NY, USA
For correspondence: zl2119@caa.columbia.edu
Bio-protocol author page: a12
,  date: 6/20/2012, 5921 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.228.

[Abstract] Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol describes a reliable method to determine the relative serum titers of anti-histone antibodies in these ...

Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-cardiolipin Antibodies

Author: Zheng Liu
Zheng LiuAffiliation: The Feinstein Institute for Medical Research, Manhasset, NY, USA
For correspondence: zl2119@caa.columbia.edu
Bio-protocol author page: a12
,  date: 6/20/2012, 6449 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.227.

[Abstract] The circulating anticardiolipin antibody is a hallmark of antiphospholipid syndrome. It also appears in a number of autoimmune mouse models and is indicative of the break of tolerance against self antigens. This protocol describes a reliable method to determine the relative serum titer of anticardiolipin in autoimmune mouse models....

Isolation of Circulating Immune Complexes from TB Patient Serum for Serodiagnosis

Authors: Uma Devi Ranganathan
Uma Devi RanganathanAffiliation: Department of Pediatrics (Infectious Diseases), Albert Einstein College of Medicine, Bronx, USA
For correspondence: krumadevi@gmail.com
Bio-protocol author page: a44
, Ramalingam Bethunaickan
Ramalingam BethunaickanAffiliation: Department of Autoimmune and Musculoskeletal Diseases, Feinstein Institute for Medical Research, Manhasset, USA
Bio-protocol author page: a24
 and Alamelu Raja
Alamelu RajaAffiliation: Department of Immunology, Tuberculosis Research Center (ICMR), Chennai, India
Bio-protocol author page: a223
,  date: 6/5/2012, 6657 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.188.

[Abstract] Estimation of circulating immune complex in tuberculosis patients has shown better insight to the infection. Isolating circulating immune complex helps quantifying both antigen and antibody in the serum. It’s a simple procedure to improve the sensitivity and specificity in serodiagnosis of tuberculosis. This protocol may be modified to detect antigen/antibody ...

Antibody Purification from Western Blotting

Author: Lin Fang
Lin FangAffiliation: Department of Pediatrics, School of Medicine, Stanford University, Stanford, USA
For correspondence: cheerfulfang@hotmail.com
Bio-protocol author page: a20
,  date: 3/20/2012, 9417 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.133.

[Abstract] This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case antigen protein solubility is limited. Disadvantage is that possible antibodies that recognize certain ...

INF-gamma Release ELISpot Assay

Author: Huagang Zhang
Huagang ZhangAffiliation: Albert Einstein College of Medicine, Yeshiva University, New York City, USA
For correspondence: huagangzhang@gmail.com
Bio-protocol author page: a21
,  date: 3/20/2012, 9504 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.120.

[Abstract] IFN-γ (Interferon-gamma) is produced mainly by activated T cells and NK cells. Production of IFN-γ (Interferon-gamma) by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ (Interferon-gamma) is typically associated with effective host defense against intracellular pathogens. The ...

[Bio101] ELISA Measurement of Mouse IL-2

Author: Guo N. Huang
Guo N. HuangAffiliation: Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, USA
For correspondence: guo.n.huang@gmail.com
Bio-protocol author page: a30
,  date: 1/5/2012, 5595 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.169.

[Abstract] Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and co-culturing conditions. Upon slightly modification and optimization, this protocol can also be adapted ...
1 2 

INF-gamma Release ELISpot Assay

Author: Huagang Zhang
Huagang ZhangAffiliation: Albert Einstein College of Medicine, Yeshiva University, New York City, USA
For correspondence: huagangzhang@gmail.com
Bio-protocol author page: a21
,  date: 3/20/2012, 9504 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.120.

[Abstract] IFN-γ (Interferon-gamma) is produced mainly by activated T cells and NK cells. Production of IFN-γ (Interferon-gamma) by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ (Interferon-gamma) is typically associated with effective ...

Antibody Purification from Western Blotting

Author: Lin Fang
Lin FangAffiliation: Department of Pediatrics, School of Medicine, Stanford University, Stanford, USA
For correspondence: cheerfulfang@hotmail.com
Bio-protocol author page: a20
,  date: 3/20/2012, 9417 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.133.

[Abstract] This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case antigen protein solubility is limited. Disadvantage ...

[Bio101] Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-dsDNA Antibodies

Author: Zheng Liu
Zheng LiuAffiliation: The Feinstein Institute for Medical Research, Manhasset, NY, USA
For correspondence: zl2119@caa.columbia.edu
Bio-protocol author page: a12
,  Anne Davidson Lab, date: 2/20/2011, 8297 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.32.

[Abstract] ELISA is an easy and relatively sensitive way to measure protein concentration. This protocol describes how to measure serum levels of murine anti-dsDNA antibodies. Circulating anti-dsDNA antibody is a hallmark of SLE both in human patients and in many SLE mouse models. This protocol was developed or ...

[Bio101] Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Total Murine Immunoglobulin

Author: Zheng Liu
Zheng LiuAffiliation: The Feinstein Institute for Medical Research, Manhasset, NY, USA
For correspondence: zl2119@caa.columbia.edu
Bio-protocol author page: a12
,  Anne Davidson Lab, date: 2/5/2011, 8234 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.33.

[Abstract] ELISA is an easy and relatively sensitive way to measure protein concentration. This protocol describes how to measure serum levels of murine total immunoglobulin of various isotypes. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research. ...

Isolation of Circulating Immune Complexes from TB Patient Serum for Serodiagnosis

Authors: Uma Devi Ranganathan
Uma Devi RanganathanAffiliation: Department of Pediatrics (Infectious Diseases), Albert Einstein College of Medicine, Bronx, USA
For correspondence: krumadevi@gmail.com
Bio-protocol author page: a44
, Ramalingam Bethunaickan
Ramalingam BethunaickanAffiliation: Department of Autoimmune and Musculoskeletal Diseases, Feinstein Institute for Medical Research, Manhasset, USA
Bio-protocol author page: a24
 and Alamelu Raja
Alamelu RajaAffiliation: Department of Immunology, Tuberculosis Research Center (ICMR), Chennai, India
Bio-protocol author page: a223
,  date: 6/5/2012, 6657 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.188.

[Abstract] Estimation of circulating immune complex in tuberculosis patients has shown better insight to the infection. Isolating circulating immune complex helps quantifying both antigen and antibody in the serum. It’s a simple procedure to improve the sensitivity and specificity in serodiagnosis of tuberculosis. ...

Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-cardiolipin Antibodies

Author: Zheng Liu
Zheng LiuAffiliation: The Feinstein Institute for Medical Research, Manhasset, NY, USA
For correspondence: zl2119@caa.columbia.edu
Bio-protocol author page: a12
,  date: 6/20/2012, 6449 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.227.

[Abstract] The circulating anticardiolipin antibody is a hallmark of antiphospholipid syndrome. It also appears in a number of autoimmune mouse models and is indicative of the break of tolerance against self antigens. This protocol describes a reliable method to determine the relative serum titer of anticardiolipin ...

[Bio101] Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies

Author: Zheng Liu
Zheng LiuAffiliation: The Feinstein Institute for Medical Research, Manhasset, NY, USA
For correspondence: zl2119@caa.columbia.edu
Bio-protocol author page: a12
,  date: 6/20/2012, 5921 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.228.

[Abstract] Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol describes a reliable method to determine the relative ...

[Bio101] ELISA Measurement of Mouse IL-2

Author: Guo N. Huang
Guo N. HuangAffiliation: Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, USA
For correspondence: guo.n.huang@gmail.com
Bio-protocol author page: a30
,  date: 1/5/2012, 5595 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.169.

[Abstract] Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and co-culturing conditions. Upon slightly modification ...

Monoclonal Antibody Purification (Nicotiana benthamiana Plants)

Authors: Adam Husk
Adam HuskAffiliation: Owensboro Cancer Research Program at James Graham Brown Cancer Center, University of Louisville School of Medicine, Owensboro, USA
Bio-protocol author page: a1126
, Krystal Teasley Hamorsky
Krystal Teasley Hamorsky Affiliation: Owensboro Cancer Research Program at James Graham Brown Cancer Center, University of Louisville School of Medicine, Owensboro, USA
Bio-protocol author page: a1127
 and Nobuyuki Matoba
Nobuyuki MatobaAffiliation: Department of Pharmacology and Toxicology, and Owensboro Cancer Research Program at James Graham Brown Cancer Center, University of Louisville School of Medicine, Owensboro, USA
For correspondence: n.matoba@louisville.edu
Bio-protocol author page: a1128
,  date: 1/20/2014, 4998 views, 1 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1034.

[Abstract] Plant-based expression systems provide an alternative biomanufacturing platform for recombinant proteins (Matoba et al., 2011). In particular, plant virus-based vectors can overexpress proteins within days in the leaf tissue of Nicotiana benthamiana (N. benthamiana). To overcome the issues of genetic ...

Pulse Chase of Suspension Cells

Authors: Lai-Yee Wong
Lai-Yee WongAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
For correspondence: laiyee.wong@usc.edu
Bio-protocol author page: a1453
, QiMing Liang
QiMing LiangAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
Bio-protocol author page: a1454
, Kevin Brulois
Kevin BruloisAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
Bio-protocol author page: a1455
 and Jae Jung
Jae JungAffiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, USA
Bio-protocol author page: a1456
,  date: 7/5/2014, 3746 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1170.

[Abstract] Pulse-chase method is a powerful technique used to follow the dynamics of proteins over a period of time. The expression level, processing, transport, secretion or half-life of proteins can be tracked by metabolically labeling the cells, such as with radiolabeled amino acids (pulse step). This protocol ...
1 2 
Immunology
Skip Navigation Links.

Hot protocols of the week
In vitro Culture of Human PBMCs
Isolation of Human PBMCs
Macrophage Inflammatory Assay
Bronchoalveolar Lavage and Lung Tissue Digestion
Protocol-In vitro T Cell Proliferation and Treg Suppression Assay with Celltrace Violet
Isolation of PBMCs Using Vacutainer® Cellular Preparation Tubes (CPTTM)
Murine Liver Myeloid Cell Isolation Protocol
ADCC Assay Protocol
Immune Cell Isolation from Mouse Femur Bone Marrow
Estimation of Wound Tissue Neutrophil and Macrophage Accumulation by Measuring Myeloperoxidase (MPO) and N-Acetyl-β-D-glucosaminidase (NAG) Activities

About Us | What's New | Author Map | Terms of Service | Copyright & IP Policy | Contact Us

© 2017 Bio-protocol LLC. ISSN: 2331-8325