Welcome guest, Sign in

Home

Biochemistry

Chromatographic Separation of the Codonocarpine Type Alkaloids from the Root Bark of Capparis decidua

Authors: Yvonne Forster
Yvonne ForsterAffiliation: Department of Chemistry, University of Zurich, Zurich, Switzerland
Bio-protocol author page: a4136
, Abdul Ghaffar
Abdul GhaffarAffiliation: Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Bio-protocol author page: a4135
 and Stefan Bienz
Stefan BienzAffiliation: Department of Chemistry, University of Zurich, Zurich, Switzerland
For correspondence: stefan.bienz@chem.uzh.ch
Bio-protocol author page: a4137
,  date: 2/20/2017, 731 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2144.

[Abstract] Various parts of the caper tree Capparis decidua have found application in traditional medicine. The isolation and structural elucidation of the codonocarpine type alkaloids contained in the root bark, however, is not trivial and has probably led to misinterpretation in the past. This protocol describes the extraction and chromatographic separation ...

Isolation and Characterization Procedure for Indole Alkaloids from the Marquesan Plant Rauvolfia Nukuhivensis

Authors: Nicolas J. Martin
Nicolas J. MartinAffiliation 1: Institut de Pharmacognosie et Phytochimie, Université de Lausanne, Lausanne, Switzerland
Affiliation 2: Laboratoire EIMS UMR 241 EIO, Université de Polynésie française, Faa’a, Tahiti, French Polynesia
For correspondence: nicolas.martin@unige.ch
Bio-protocol author page: a2647
, Maël Nicolas
Maël NicolasAffiliation: Laboratoire EIMS UMR 241 EIO, Université de Polynésie française, Faa’a, Tahiti, French Polynesia
Bio-protocol author page: a2648
, Gaël Lecellier
Gaël LecellierAffiliation: Laboratoire d’Excellence ‘‘CORAIL’’-Centre de Recherches Insulaires et Observatoire de l’Environnement (CRIOBE), Papetoai, Moorea, French Polynesia
Bio-protocol author page: a2649
 and Phila Raharivelomanana
Phila RaharivelomananaAffiliation: Laboratoire EIMS UMR 241 EIO, Université de Polynésie française, Faa’a, Tahiti, French Polynesia
For correspondence: phila.raharivelomanana@upf.pf
Bio-protocol author page: a2650
,  date: 10/20/2015, 2220 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1625.

[Abstract] A plethora of natural products, mostly secondary metabolites, are isolated and purified from many different organisms, like plants, fungi, algae, marine invertebrates, etc. The extraction procedure is specific to each organism, but some guidelines are usually followed for any purification procedure regarding targeted metabolites, such as alkaloids. ...

Paper Chromatography as Exemplified by Separation of Urocanic Acid and Deaminohistidine

Author: Alexander V. Bogachev
Alexander V. BogachevAffiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
For correspondence: bogachev@belozersky.msu.ru
Bio-protocol author page: a487
,  date: 6/20/2013, 3228 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.797.

[Abstract] Paper chromatography is an ancient technique to separate low molecular mass compounds based on their distribution between mobile phase (solvent) and stationary phase (cellulose and cellulose-bound water). Paper chromatography has been largely replaced by thin layer chromatography and high performance liquid chromatography as the latter methods have ...

Determination of Nectar Nicotine Concentration in N. attenuata

Authors: Eva Rothe
Eva RotheAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page: a578
, Matthias Schöttner
Matthias SchöttnerAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page: a579
, Danny Kessler
Danny KesslerAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page: a580
 and Ian T. Baldwin
Ian T. BaldwinAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
For correspondence: baldwin@ice.mpg.de
Bio-protocol author page: a581
,  date: 4/20/2013, 3997 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.451.

[Abstract] In this protocol, the determination of the nicotine concentration in nectar of Nicotiana attenuata is described. This method is applicable for the investigation of small amounts of nectar (above 1 μl). It is a high-throughput protocol optimized and streamlined for one skilled person to process approximately 100 nectar samples per day....
1 

Determination of Nectar Nicotine Concentration in N. attenuata

Authors: Eva Rothe
Eva RotheAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page: a578
, Matthias Schöttner
Matthias SchöttnerAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page: a579
, Danny Kessler
Danny KesslerAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
Bio-protocol author page: a580
 and Ian T. Baldwin
Ian T. BaldwinAffiliation: Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena, Germany
For correspondence: baldwin@ice.mpg.de
Bio-protocol author page: a581
,  date: 4/20/2013, 3997 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.451.

[Abstract] In this protocol, the determination of the nicotine concentration in nectar of Nicotiana attenuata is described. This method is applicable for the investigation of small amounts of nectar (above 1 μl). It is a high-throughput protocol optimized and streamlined for one skilled person to process approximately ...

Paper Chromatography as Exemplified by Separation of Urocanic Acid and Deaminohistidine

Author: Alexander V. Bogachev
Alexander V. BogachevAffiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
For correspondence: bogachev@belozersky.msu.ru
Bio-protocol author page: a487
,  date: 6/20/2013, 3228 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.797.

[Abstract] Paper chromatography is an ancient technique to separate low molecular mass compounds based on their distribution between mobile phase (solvent) and stationary phase (cellulose and cellulose-bound water). Paper chromatography has been largely replaced by thin layer chromatography and high performance ...

Isolation and Characterization Procedure for Indole Alkaloids from the Marquesan Plant Rauvolfia Nukuhivensis

Authors: Nicolas J. Martin
Nicolas J. MartinAffiliation 1: Institut de Pharmacognosie et Phytochimie, Université de Lausanne, Lausanne, Switzerland
Affiliation 2: Laboratoire EIMS UMR 241 EIO, Université de Polynésie française, Faa’a, Tahiti, French Polynesia
For correspondence: nicolas.martin@unige.ch
Bio-protocol author page: a2647
, Maël Nicolas
Maël NicolasAffiliation: Laboratoire EIMS UMR 241 EIO, Université de Polynésie française, Faa’a, Tahiti, French Polynesia
Bio-protocol author page: a2648
, Gaël Lecellier
Gaël LecellierAffiliation: Laboratoire d’Excellence ‘‘CORAIL’’-Centre de Recherches Insulaires et Observatoire de l’Environnement (CRIOBE), Papetoai, Moorea, French Polynesia
Bio-protocol author page: a2649
 and Phila Raharivelomanana
Phila RaharivelomananaAffiliation: Laboratoire EIMS UMR 241 EIO, Université de Polynésie française, Faa’a, Tahiti, French Polynesia
For correspondence: phila.raharivelomanana@upf.pf
Bio-protocol author page: a2650
,  date: 10/20/2015, 2220 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1625.

[Abstract] A plethora of natural products, mostly secondary metabolites, are isolated and purified from many different organisms, like plants, fungi, algae, marine invertebrates, etc. The extraction procedure is specific to each organism, but some guidelines are usually followed for any purification procedure ...

Chromatographic Separation of the Codonocarpine Type Alkaloids from the Root Bark of Capparis decidua

Authors: Yvonne Forster
Yvonne ForsterAffiliation: Department of Chemistry, University of Zurich, Zurich, Switzerland
Bio-protocol author page: a4136
, Abdul Ghaffar
Abdul GhaffarAffiliation: Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Bio-protocol author page: a4135
 and Stefan Bienz
Stefan BienzAffiliation: Department of Chemistry, University of Zurich, Zurich, Switzerland
For correspondence: stefan.bienz@chem.uzh.ch
Bio-protocol author page: a4137
,  date: 2/20/2017, 731 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2144.

[Abstract] Various parts of the caper tree Capparis decidua have found application in traditional medicine. The isolation and structural elucidation of the codonocarpine type alkaloids contained in the root bark, however, is not trivial and has probably led to misinterpretation in the past. This protocol describes ...
1 
Biochemistry
Skip Navigation Links.

Hot protocols of the week
Dimethylmethylene Blue Assay (DMMB)
ImmunoPrecipitation of Nuclear Protein with Antibody Affinity Columns
Blood AST, ALT and UREA/BUN Level Analysis
Ubiquitination Assay for Mammalian Cells
In vitro RNA-protein Binding Assay by UV Crosslinking
Protein-peptide Interaction by Surface Plasmon Resonance
Measuring Oxidative Stress in Caenorhabditis elegans: Paraquat and Juglone Sensitivity Assays
Glucose Production Assay in Primary Mouse Hepatocytes
Cross-linked RNA Immunoprecipitation
Lipid Extraction from Mouse Feces

About Us | What's New | Author Map | Terms of Service | Copyright & IP Policy | Contact Us

© 2017 Bio-protocol LLC. ISSN: 2331-8325