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Biochemistry

Determination of Intra- and Extracellular Glucose in Mycelium of Fusarium oxysporum

Authors: Carmen Ruiz-Roldan
Carmen Ruiz-RoldanAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
For correspondence: ge2rurom@uco.es
Bio-protocol author page: a3318
 and M. Isabel G. Roncero
M. Isabel G. RonceroAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
Bio-protocol author page: a3319
,  date: 7/20/2016, 1509 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1868.

[Abstract] To study alterations in the metabolism and/or in the transport of glucose during Fusarium oxysporum vegetative growth, we determined intracellular glucose levels in different fungal strains, as well as the amount of glucose remaining in the supernatants after growth in synthetic medium (SM) supplemented with either 0.05 or 2.5% glucose. We used the ...

13C Tracer Studies of Metabolism in Mouse Tumor Xenografts

Authors: Andrew N. Lane
Andrew N. LaneAffiliation: Center for Environmental Systems Biochemistry, Dept. Toxicology and Cancer Biology and Markey Cancer Center, Lexington, USA
For correspondence: Andrew.lane@uky.edu
Bio-protocol author page: a2708
, Jun Yan
Jun YanAffiliation: JG Brown Cancer Center, University of Louisville, Clinical & Translational Research Building, University of Louisville, Louisville, USA
Bio-protocol author page: a2709
 and Teresa W-M. Fan
Teresa W-M. FanAffiliation: Center for Environmental Systems Biochemistry, Dept. Toxicology and Cancer Biology and Markey Cancer Center, Lexington, USA
Bio-protocol author page: a2710
,  date: 11/20/2015, 2316 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1650.

[Abstract] Mice are widely used for human tumor xenograft studies of cancer development and drug efficacy and toxicity. Stable isotope tracing coupled with metabolomic analysis is an emerging approach for assaying metabolic network activity. In mouse models there are several routes of tracer introduction, which have particular advantages and disadvantages that ...

Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling

Authors: Zhijin Zhang
Zhijin ZhangAffiliation: Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, China
Bio-protocol author page: a684
 and Rongfeng Huang
Rongfeng HuangAffiliation: Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, China
For correspondence: rfhuang@caas.cn
Bio-protocol author page: a362
,  date: 7/20/2013, 11118 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.817.

[Abstract] The protocol has four sub-protocols, which are about the measurement of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content, respectively, in Arabidopsis seedling by using spectrophotometer. These methods are simple, effective and reproducible, which will help the researchers who are not familiar with these approaches, quickly ...

Glucose Production Assay in Primary Mouse Hepatocytes

Authors: Michihiro Matsumoto
Michihiro MatsumotoAffiliation: Department of Molecular Metabolic Regulation, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
For correspondence: mmatsumoto@ri.ncgm.go.jp
Bio-protocol author page: a145
 and Mashito Sakai
Mashito SakaiAffiliation: Department of Molecular Metabolic Regulation, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
Bio-protocol author page: a146
,  date: 11/5/2012, 10001 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.284.

[Abstract] Hepatic glucose production is a primary determinant of fasting hyperglycemia in type 2 diabetic patients. Glucagon-cAMP-PKA pathway increases, but insulin-PI3 kinase-Akt pathway suppresses glucose production. This assay aims to evaluate the ability of isolated mouse hepatocytes to release newly synthesized glucose mainly from lactate and pyruvate as ...

5-Hydroxymethylcytosine (5-hmC) Specific Enrichment

Authors: Keith E Szulwach
Keith E SzulwachAffiliation: Department of Human Genetics, Emory University School of Medicine, Atlanta, USA
Bio-protocol author page: a69
, Chun-Xiao Song
Chun-Xiao SongAffiliation: Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago, Chicago, USA
Bio-protocol author page: a70
, Chuan He
Chuan HeAffiliation: Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago, Chicago, USA
Bio-protocol author page: a71
 and Peng Jin
Peng JinAffiliation: Department of Human Genetics, Emory University School of Medicine, Atlanta, USA
For correspondence: peng.jin@emory.edu
Bio-protocol author page: a72
,  date: 8/5/2012, 4379 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.242.

[Abstract] 5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via next-generation ...

[Bio101] Glucose Tolerance Test in Mice

Author: Peichuan Zhang
Peichuan ZhangAffiliation 1: Department of Biology, The Pennsylvania State University, University Park, PA, USA
Affiliation 2: Department of Biochemistry and Biophysics, University of California, San Francisco, USA
For correspondence: peichuan.zhang@ucsf.edu
Bio-protocol author page: a11
,  date: 10/5/2011, 31970 views, 4 Q&A
DOI: https://doi.org/10.21769/BioProtoc.159.

[Abstract] Glucose tolerance test is a standard procedure that addresses how quickly exogenous glucose can be cleared from blood. Specifically, uptake of glucose from the blood by cells is regulated by insulin. Impairment of glucose tolerance (i.e, longer time to clear given amount of glucose) indicates problems with maintenance of glucose homeostasis (insulin ...
1 

[Bio101] Glucose Tolerance Test in Mice

Author: Peichuan Zhang
Peichuan ZhangAffiliation 1: Department of Biology, The Pennsylvania State University, University Park, PA, USA
Affiliation 2: Department of Biochemistry and Biophysics, University of California, San Francisco, USA
For correspondence: peichuan.zhang@ucsf.edu
Bio-protocol author page: a11
,  date: 10/5/2011, 31970 views, 4 Q&A
DOI: https://doi.org/10.21769/BioProtoc.159.

[Abstract] Glucose tolerance test is a standard procedure that addresses how quickly exogenous glucose can be cleared from blood. Specifically, uptake of glucose from the blood by cells is regulated by insulin. Impairment of glucose tolerance (i.e, longer time to clear given amount of glucose) indicates problems ...

Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling

Authors: Zhijin Zhang
Zhijin ZhangAffiliation: Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, China
Bio-protocol author page: a684
 and Rongfeng Huang
Rongfeng HuangAffiliation: Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, China
For correspondence: rfhuang@caas.cn
Bio-protocol author page: a362
,  date: 7/20/2013, 11118 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.817.

[Abstract] The protocol has four sub-protocols, which are about the measurement of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content, respectively, in Arabidopsis seedling by using spectrophotometer. These methods are simple, effective and reproducible, which will help the researchers ...

Glucose Production Assay in Primary Mouse Hepatocytes

Authors: Michihiro Matsumoto
Michihiro MatsumotoAffiliation: Department of Molecular Metabolic Regulation, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
For correspondence: mmatsumoto@ri.ncgm.go.jp
Bio-protocol author page: a145
 and Mashito Sakai
Mashito SakaiAffiliation: Department of Molecular Metabolic Regulation, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
Bio-protocol author page: a146
,  date: 11/5/2012, 10001 views, 2 Q&A
DOI: https://doi.org/10.21769/BioProtoc.284.

[Abstract] Hepatic glucose production is a primary determinant of fasting hyperglycemia in type 2 diabetic patients. Glucagon-cAMP-PKA pathway increases, but insulin-PI3 kinase-Akt pathway suppresses glucose production. This assay aims to evaluate the ability of isolated mouse hepatocytes to release newly synthesized ...

5-Hydroxymethylcytosine (5-hmC) Specific Enrichment

Authors: Keith E Szulwach
Keith E SzulwachAffiliation: Department of Human Genetics, Emory University School of Medicine, Atlanta, USA
Bio-protocol author page: a69
, Chun-Xiao Song
Chun-Xiao SongAffiliation: Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago, Chicago, USA
Bio-protocol author page: a70
, Chuan He
Chuan HeAffiliation: Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago, Chicago, USA
Bio-protocol author page: a71
 and Peng Jin
Peng JinAffiliation: Department of Human Genetics, Emory University School of Medicine, Atlanta, USA
For correspondence: peng.jin@emory.edu
Bio-protocol author page: a72
,  date: 8/5/2012, 4379 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.242.

[Abstract] 5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could ...

13C Tracer Studies of Metabolism in Mouse Tumor Xenografts

Authors: Andrew N. Lane
Andrew N. LaneAffiliation: Center for Environmental Systems Biochemistry, Dept. Toxicology and Cancer Biology and Markey Cancer Center, Lexington, USA
For correspondence: Andrew.lane@uky.edu
Bio-protocol author page: a2708
, Jun Yan
Jun YanAffiliation: JG Brown Cancer Center, University of Louisville, Clinical & Translational Research Building, University of Louisville, Louisville, USA
Bio-protocol author page: a2709
 and Teresa W-M. Fan
Teresa W-M. FanAffiliation: Center for Environmental Systems Biochemistry, Dept. Toxicology and Cancer Biology and Markey Cancer Center, Lexington, USA
Bio-protocol author page: a2710
,  date: 11/20/2015, 2316 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1650.

[Abstract] Mice are widely used for human tumor xenograft studies of cancer development and drug efficacy and toxicity. Stable isotope tracing coupled with metabolomic analysis is an emerging approach for assaying metabolic network activity. In mouse models there are several routes of tracer introduction, which ...

Determination of Intra- and Extracellular Glucose in Mycelium of Fusarium oxysporum

Authors: Carmen Ruiz-Roldan
Carmen Ruiz-RoldanAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
For correspondence: ge2rurom@uco.es
Bio-protocol author page: a3318
 and M. Isabel G. Roncero
M. Isabel G. RonceroAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
Bio-protocol author page: a3319
,  date: 7/20/2016, 1509 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1868.

[Abstract] To study alterations in the metabolism and/or in the transport of glucose during Fusarium oxysporum vegetative growth, we determined intracellular glucose levels in different fungal strains, as well as the amount of glucose remaining in the supernatants after growth in synthetic medium (SM) supplemented ...
1 
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