Immunology

Isolation and Infection of Drosophila Primary Hemocytes

Authors: Charles Tracy
Charles TracyAffiliation: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Bio-protocol author page: a4450
and Helmut Krämer
Helmut KrämerAffiliation 1: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Affiliation 2: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Affiliation 3: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
For correspondence: helmut.kramer@utsouthwestern.edu
Bio-protocol author page: a4451
, date: 6/5/2017, 317 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2300.

[Abstract] Phagocytosis of invading pathogens and their subsequent clearance in lysosomes is important for organismal fitness. We have devised the following protocol to extract phagocytic hemocytes from wild-type and mutant Drosophila larvae and infect the isolated hemocytes with GFP-labeled E. coli to measure the rate of phagocytosis and degradation within individual ...

Escherichia coli Infection of Drosophila

Authors: Charles Tracy
Charles TracyAffiliation: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, USA
Bio-protocol author page: a4450
and Helmut Krämer
Helmut KrämerAffiliation 1: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, USA
Affiliation 2: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, USA
For correspondence: helmut.kramer@utsouthwestern.edu
Bio-protocol author page: a4451
, date: 5/5/2017, 429 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2256.

[Abstract] Following septic insults, healthy insects, just like vertebrates, mount a complex immune response to contain and destroy pathogens. The failure to efficiently clear bacterial infections in immuno-compromised fly mutants leads to higher mortality rates which provide a powerful indicator for genes with important roles in innate immunity. The following ...

Escherichia coli Infection of Drosophila

Authors: Charles Tracy
Charles TracyAffiliation: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, USA
Bio-protocol author page: a4450
and Helmut Krämer
Helmut KrämerAffiliation 1: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, USA
Affiliation 2: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, USA
For correspondence: helmut.kramer@utsouthwestern.edu
Bio-protocol author page: a4451
, date: 5/5/2017, 429 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2256.

Isolation and Infection of Drosophila Primary Hemocytes

Authors: Charles Tracy
Charles TracyAffiliation: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Bio-protocol author page: a4450
and Helmut Krämer
Helmut KrämerAffiliation 1: Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Affiliation 2: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Affiliation 3: Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
For correspondence: helmut.kramer@utsouthwestern.edu
Bio-protocol author page: a4451
, date: 6/5/2017, 317 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2300.

Immunology

Animal model

Drosophila (Fruit fly)

Immune cell differentiation

Immune cell function

Immune cell imaging

Immune cell isolation

Immune cell staining

Inflammatory disorder

Mucosal immunology

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