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In vitro Cell Wall Stress Assay for Fusarium oxysporum

Authors: Elena Pérez-Nadales
Elena Pérez-NadalesAffiliation: Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Reina Sofia University Hospital, University of Cordoba, Spain
For correspondence: elena.pereznadales@imibic.org
Bio-protocol author page: a3465
 and Antonio Di Pietro
Antonio Di PietroAffiliation: Departamento de Genética, Campus de Excelencia Agroalimentario ceiA3, Universidad de Cordoba, Spain
Bio-protocol author page: a3466
date: 9/5/2016, 1427 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1915.

[Abstract] In this protocol we describe a cell wall stress assay for the fungal pathogen F. oxysporum, based on exposure to the two anionic dyes Calcofluor White (CFW) and Congo Red (CR). Both compounds have been used to exert stress upon the fungal cell wall in vitro (Perez-Nadales and Di Pietro, 2015; Perez-Nadales ...

Quantification of Chitinase Activity in Fusarium oxysporum

Authors: Carmen Ruiz-Roldan
Carmen Ruiz-RoldanAffiliation: Departamento de Genetica, Universidad de Cordoba, Spain; Campus de Excelencia Agroalimentario, Cordoba, Spain
For correspondence: ge2rurom@uco.es
Bio-protocol author page: a3318
 and M. Isabel G. Roncero
M. Isabel G. RonceroAffiliation: Departamento de Genetica, Universidad de Cordoba, Spain; Campus de Excelencia Agroalimentario, Cordoba, Spain
Bio-protocol author page: a3319
date: 8/5/2016, 1616 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1882.

[Abstract] Fungal morphogenetic development requires modification and plasticity of the cell wall, which implies synthesis and remodelling of its components, including chitin and glucan. Thus chitinase and glucanase activities are crucial for cell-wall biogenesis and cell division. Quantification of chitinase ...

Determination of Intracellular ATP Levels in Mycelium of Fusarium oxysporum

Authors: Carmen Ruiz-Roldan
Carmen Ruiz-RoldanAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
For correspondence: ge2rurom@uco.es
Bio-protocol author page: a3318
 and M. Isabel G. Roncero
M. Isabel G. RonceroAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
Bio-protocol author page: a3319
date: 7/20/2016, 1396 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1869.

[Abstract] Glycolysis provides metabolites for energy production via oxidative phosphorylation during vegetative growth of Fusarium oxysporum. Therefore, determination of intracellular ATP levels might be of valuable help to analyze regulation of glycolysis/gluconeogenesis pathways. The protocol described here ...
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Quantification of Chitinase Activity in Fusarium oxysporum

Authors: Carmen Ruiz-Roldan
Carmen Ruiz-RoldanAffiliation: Departamento de Genetica, Universidad de Cordoba, Spain; Campus de Excelencia Agroalimentario, Cordoba, Spain
For correspondence: ge2rurom@uco.es
Bio-protocol author page: a3318
 and M. Isabel G. Roncero
M. Isabel G. RonceroAffiliation: Departamento de Genetica, Universidad de Cordoba, Spain; Campus de Excelencia Agroalimentario, Cordoba, Spain
Bio-protocol author page: a3319
date: 8/5/2016, 1616 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1882.

[Abstract] Fungal morphogenetic development requires modification and plasticity of the cell wall, which implies synthesis and remodelling of its components, including chitin and glucan. Thus chitinase and glucanase activities are crucial for cell-wall biogenesis and cell division. Quantification of chitinase ...

In vitro Cell Wall Stress Assay for Fusarium oxysporum

Authors: Elena Pérez-Nadales
Elena Pérez-NadalesAffiliation: Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Reina Sofia University Hospital, University of Cordoba, Spain
For correspondence: elena.pereznadales@imibic.org
Bio-protocol author page: a3465
 and Antonio Di Pietro
Antonio Di PietroAffiliation: Departamento de Genética, Campus de Excelencia Agroalimentario ceiA3, Universidad de Cordoba, Spain
Bio-protocol author page: a3466
date: 9/5/2016, 1427 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1915.

[Abstract] In this protocol we describe a cell wall stress assay for the fungal pathogen F. oxysporum, based on exposure to the two anionic dyes Calcofluor White (CFW) and Congo Red (CR). Both compounds have been used to exert stress upon the fungal cell wall in vitro (Perez-Nadales and Di Pietro, 2015; Perez-Nadales ...

Determination of Intracellular ATP Levels in Mycelium of Fusarium oxysporum

Authors: Carmen Ruiz-Roldan
Carmen Ruiz-RoldanAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
For correspondence: ge2rurom@uco.es
Bio-protocol author page: a3318
 and M. Isabel G. Roncero
M. Isabel G. RonceroAffiliation: Departamento de Genetica, Universidad de Cordoba, Campus de Excelencia Agroalimentario (ceiA3), Cordoba, Spain
Bio-protocol author page: a3319
date: 7/20/2016, 1396 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1869.

[Abstract] Glycolysis provides metabolites for energy production via oxidative phosphorylation during vegetative growth of Fusarium oxysporum. Therefore, determination of intracellular ATP levels might be of valuable help to analyze regulation of glycolysis/gluconeogenesis pathways. The protocol described here ...
1 
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