Bacteria

Preparation of Genomic Overexpression Library

Author: Wei-Yun (Winnie) Wholey
Wei-Yun (Winnie) WholeyAffiliation: Pediatrics and Communicable Diseases, University of Michigan, Medical School, Ann Arbor, MI, USA
For correspondence: weiyun@umich.edu
Bio-protocol author page: a126
, date: 10/5/2012, 4619 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.271.

[Abstract] This protocol is used to identify/select for Escherichia coli genes that, when overexpressed in Vibrio cholerae Hsp33 (hslO gene) deletion mutant, protect against oxidative heat stress and, by extension, against HOCl-mediated protein damage. In the referenced publication, I found that V. cholerae mutant ...

	Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
	Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins
	Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
	Pyocyanin Extraction and Quantitative Analysis in Swarming Pseudomonas aeruginosa
	Gentamicin Protection Assay to Determine Bacterial Survival within Macrophages
	RNA Isolation from Synechocystis
	Isolation of Rhizosphere Bacterial Communities from Soil
	In vitro RNA-protein Binding Assay by UV Crosslinking
	Protein-peptide Interaction by Surface Plasmon Resonance
	Heterologous Expression and Purification of the Magnesium Transporter A (MgtA) in Escherichia coli