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MNase Digestion for Nucleosome Mapping in Neurospora
Authors:
Cigdem Sancar
Cigdem Sancar
Affiliation:
Center for Circadian Biology, California Center for Algal Biotechnology, Division of Biological Sciences, University of California, San Diego, USA
Bio-protocol author page:
a3166
,
Gencer Sancar
Gencer Sancar
Affiliation:
Gene Expression Laboratory, Salk Institute, San Diego, USA
Bio-protocol author page:
a3167
and
Michael Brunner
Michael Brunner
Affiliation:
Biochemistry Center, University of Heidelberg, Heidelberg, Germany
For correspondence:
michael.brunner@bzh.uni-heidelberg.de
Bio-protocol author page:
a3168
,
date: 6/5/2016, 1463 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1825
.
[Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus
Neurospora crassa
chromatin ...
1
MNase Digestion for Nucleosome Mapping in Neurospora
Authors:
Cigdem Sancar
Cigdem Sancar
Affiliation:
Center for Circadian Biology, California Center for Algal Biotechnology, Division of Biological Sciences, University of California, San Diego, USA
Bio-protocol author page:
a3166
,
Gencer Sancar
Gencer Sancar
Affiliation:
Gene Expression Laboratory, Salk Institute, San Diego, USA
Bio-protocol author page:
a3167
and
Michael Brunner
Michael Brunner
Affiliation:
Biochemistry Center, University of Heidelberg, Heidelberg, Germany
For correspondence:
michael.brunner@bzh.uni-heidelberg.de
Bio-protocol author page:
a3168
,
date: 6/5/2016, 1463 views,
0 Q&A
DOI:
https://doi.org/10.21769/BioProtoc.1825
.
[Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus
Neurospora crassa
chromatin ...
1
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