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Functional ex-vivo Imaging of Arterial Cellular Recruitment and Lipid Extravasation

Authors: Emiel P.C. van der Vorst
Emiel P.C. van der VorstAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
For correspondence: Emiel.Van_der_Vorst@med.uni-muenchen.de
Bio-protocol author page: a4681
, Sanne L. Maas
Sanne L. MaasAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4682
, Almudena Ortega-Gomez
Almudena Ortega-GomezAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4683
, Jeroen M.M. Hameleers
Jeroen M.M. HameleersAffiliation: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Bio-protocol author page: a4684
, Mariaelvy Bianchini
Mariaelvy BianchiniAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4685
, Yaw Asare
Yaw AsareAffiliation: Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4686
, Oliver Soehnlein
Oliver SoehnleinAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany
Affiliation 3: Department of Physiology and Pharmacology (FyFa), Karolinska Institutet, Stockholm, Sweden
Bio-protocol author page: a4687
, Yvonne Döring
Yvonne DöringAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4688
, Christian Weber
Christian WeberAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Affiliation 3: DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany
Bio-protocol author page: a3209
 and Remco T.A. Megens
Remco T.A. MegensAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
For correspondence: Remco.Megens@med.uni-muenchen.de
Bio-protocol author page: a4689
,  date: 6/20/2017, 269 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2344.

[Abstract] The main purpose of this sophisticated and highly versatile method is to visualize and quantify structural vessel wall properties, cellular recruitment, and lipid/dextran extravasation under physiological conditions in living arteries. This will be of interest for a broad range of researchers within ...

Aorta Atherosclerosis Lesion Analysis in Hyperlipidemic Mice

Authors: Sarajo Mohanta
Sarajo MohantaAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
For correspondence: Sarajo.Mohanta@med.uni-muenchen.de
Bio-protocol author page: a3207
, Changjun Yin
Changjun YinAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3208
, Christian Weber
Christian WeberAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3209
, Desheng Hu
Desheng HuAffiliation 1: Institute of Molecular Immunology, Helmholtz Zentrum München, Marchioninistrasse, Munich, Germany
Affiliation 2: Institute for Immunology, Ludwig-Maximilians-University, Großhadernerstrasse, Munich, Germany
Bio-protocol author page: a3210
 and Andreas J. R. Habenicht
Andreas J. R. HabenichtAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3211
,  date: 6/5/2016, 2638 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1833.

[Abstract] Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE-/-) mice are used as experimental models to study human atherosclerosis. ApoE-/- mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various ...
1 

Aorta Atherosclerosis Lesion Analysis in Hyperlipidemic Mice

Authors: Sarajo Mohanta
Sarajo MohantaAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
For correspondence: Sarajo.Mohanta@med.uni-muenchen.de
Bio-protocol author page: a3207
, Changjun Yin
Changjun YinAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3208
, Christian Weber
Christian WeberAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3209
, Desheng Hu
Desheng HuAffiliation 1: Institute of Molecular Immunology, Helmholtz Zentrum München, Marchioninistrasse, Munich, Germany
Affiliation 2: Institute for Immunology, Ludwig-Maximilians-University, Großhadernerstrasse, Munich, Germany
Bio-protocol author page: a3210
 and Andreas J. R. Habenicht
Andreas J. R. HabenichtAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Pettenkoferstrasse, Munich, Germany
Bio-protocol author page: a3211
,  date: 6/5/2016, 2638 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1833.

[Abstract] Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE-/-) mice are used as experimental models to study human atherosclerosis. ApoE-/- mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various ...

Functional ex-vivo Imaging of Arterial Cellular Recruitment and Lipid Extravasation

Authors: Emiel P.C. van der Vorst
Emiel P.C. van der VorstAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
For correspondence: Emiel.Van_der_Vorst@med.uni-muenchen.de
Bio-protocol author page: a4681
, Sanne L. Maas
Sanne L. MaasAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4682
, Almudena Ortega-Gomez
Almudena Ortega-GomezAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4683
, Jeroen M.M. Hameleers
Jeroen M.M. HameleersAffiliation: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Bio-protocol author page: a4684
, Mariaelvy Bianchini
Mariaelvy BianchiniAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4685
, Yaw Asare
Yaw AsareAffiliation: Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4686
, Oliver Soehnlein
Oliver SoehnleinAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany
Affiliation 3: Department of Physiology and Pharmacology (FyFa), Karolinska Institutet, Stockholm, Sweden
Bio-protocol author page: a4687
, Yvonne Döring
Yvonne DöringAffiliation: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Bio-protocol author page: a4688
, Christian Weber
Christian WeberAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
Affiliation 3: DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany
Bio-protocol author page: a3209
 and Remco T.A. Megens
Remco T.A. MegensAffiliation 1: Institute for Cardiovascular Prevention (IPEK), Klinikum der Universität München, Ludwig-Maximilians-University, Munich, Germany
Affiliation 2: Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, the Netherlands
For correspondence: Remco.Megens@med.uni-muenchen.de
Bio-protocol author page: a4689
,  date: 6/20/2017, 269 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2344.

[Abstract] The main purpose of this sophisticated and highly versatile method is to visualize and quantify structural vessel wall properties, cellular recruitment, and lipid/dextran extravasation under physiological conditions in living arteries. This will be of interest for a broad range of researchers within ...
1 
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