Mammalia

Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta

Authors: Callie S. Kwartler
Callie S. KwartlerAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3821
, Ping Zhou
Ping ZhouAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3822
, Shao-Qing Kuang
Shao-Qing KuangAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3823
, Xue-Yan Duan
Xue-Yan DuanAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3824
, Limin Gong
Limin GongAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
Bio-protocol author page: a3825
and Dianna M. Milewicz
Dianna M. MilewiczAffiliation: Department of Internal Medicine, Division of Medical Genetics, University of Texas Health Science Center, Houston, Texas, USA
For correspondence: Dianna.M.Milewicz@uth.tmc.edu
Bio-protocol author page: a3827
, date: 12/5/2016, 1685 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2045.

[Abstract] Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed ...

Preparation of Single Cell Suspensions from Mouse Aorta

Authors: Desheng Hu
Desheng HuAffiliation 1: Institute for Immunology, Ludwig-Maximilians-University Munich, Munich, Germany
Affiliation 2: Institute of Molecular Immunology, Helmholtz Zentrum Munich, Munich, Germany
For correspondence: desheng.hu@med.uni-muenchen.de
Bio-protocol author page: a3210
, Changjun Yin
Changjun YinAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3208
, Sarajo Mohanta
Sarajo MohantaAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3207
, Christian Weber
Christian WeberAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3209
and Andreas J. R. Habenicht
Andreas J. R. HabenichtAffiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University Munich, Munich, Germany
Bio-protocol author page: a3211
, date: 6/5/2016, 2206 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1832.

[Abstract] Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery ...

	In vitro Culture of Human PBMCs
	Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
	Analysis of Protein Stability by the Cycloheximide Chase Assay
	Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
	Dimethylmethylene Blue Assay (DMMB)
	Isolation of Human PBMCs
	BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
	Analysis of Intestinal Permeability in Mice
	Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
	Subcellular Fractionation of Cultured Human Cell Lines