Mammalia

Stereotaxic Surgery for Suprachiasmatic Nucleus Lesions in Mice

Authors: Kimiko Shimizu
Kimiko ShimizuAffiliation: Department of Biological Sciences, School of Science, the University of Tokyo, Tokyo, 113-0033 Japan
For correspondence: shimizuk@bs.s.u-tokyo.ac.jp
Bio-protocol author page: a4698
and Yoshitaka Fukada
Yoshitaka FukadaAffiliation: Department of Biological Sciences, School of Science, the University of Tokyo, Tokyo, 113-0033 Japan
Bio-protocol author page: a4699
, date: 6/20/2017, 198 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2346.

[Abstract] Site-specific lesions are invaluable methods for investigating the function of brain regions within the central nervous system and can be used to study neural mechanisms of behaviors. Precise stereotaxic surgery is required to lesion small regions of the brain such as the suprachiasmatic nucleus (SCN), ...

Olfactory Cued Learning Paradigm

Authors: Gary Liu
Gary LiuAffiliation 1: Program in Developmental Biology, Baylor College of Medicine, Houston, USA
Affiliation 2: Medical Scientist Training Program, Baylor College of Medicine, Houston, USA
For correspondence: garyliu87@gmail.com
Bio-protocol author page: a4435
, Cynthia K. McClard*
Cynthia K. McClardAffiliation 1: Medical Scientist Training Program, Baylor College of Medicine, Houston, USA
Affiliation 2: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4436
, Burak Tepe*
Burak TepeAffiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4437
, Jessica Swanson
Jessica SwansonAffiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4438
, Brandon Pekarek
Brandon PekarekAffiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4439
, Sugi Panneerselvam
Sugi PanneerselvamAffiliation: Rice University, Houston, USA
Bio-protocol author page: a4440
and Benjamin R. Arenkiel
Benjamin R. ArenkielAffiliation 1: Program in Developmental Biology, Baylor College of Medicine, Houston, USA
Affiliation 2: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Affiliation 3: Department of Neuroscience, Baylor College of Medicine, Houston, USA
Affiliation 4: Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, USA
Bio-protocol author page: a4441
(*contributed equally to this work), date: 5/5/2017, 342 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2251.

[Abstract] Sensory stimulation leads to structural changes within the CNS (Central Nervous System), thus providing the fundamental mechanism for learning and memory. The olfactory circuit offers a unique model for studying experience-dependent plasticity, partly due to a continuous supply of integrating adult ...

The Object Context-place-location Paradigm for Testing Spatial Memory in Mice

Authors: Edith Lesburguères
Edith LesburguèresAffiliation: Center for Neural Science, New York University, New York, USA
Bio-protocol author page: a4246
, Panayiotis Tsokas
Panayiotis TsokasAffiliation 1: Department of Physiology and Pharmacology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Affiliation 2: Department of Anesthesiology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Bio-protocol author page: a4243
, Todd Charlton Sacktor
Todd Charlton SacktorAffiliation 1: Department of Physiology and Pharmacology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Affiliation 2: Department of Neurology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Bio-protocol author page: a4244
and André Antonio Fenton
André Antonio FentonAffiliation 1: Center for Neural Science, New York University, New York, USA
Affiliation 2: Department of Physiology and Pharmacology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
For correspondence: afenton@nyu.edu
Bio-protocol author page: a4245
, date: 4/20/2017, 525 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2231.

[Abstract] This protocol was originally designed to examine long-term spatial memory in PKMζ knockout (i.e., PKMζ-null) mice (Tsokas et al., 2016). Our main goal was to test whether the ability of these animals to maintain previously acquired spatial information was sensitive to the type and complexity of the ...

Delayed Spatial Win-shift Test on Radial Arm Maze

Authors: Simone N. De Luca
Simone N. De LucaAffiliation: School of Health and Biomedical Sciences, RMIT University, Melbourne, Vic, Australia
Bio-protocol author page: a3841
, Luba Sominsky
Luba Sominsky Affiliation: School of Health and Biomedical Sciences, RMIT University, Melbourne, Vic, Australia
Bio-protocol author page: a3842
and Sarah J. Spencer
Sarah J. SpencerAffiliation: School of Health and Biomedical Sciences, RMIT University, Melbourne, Vic, Australia
For correspondence: Sarah.Spencer@rmit.edu.au
Bio-protocol author page: a3843
, date: 12/5/2016, 1145 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2053.

[Abstract] The radial arm maze (RAM) is used to assess reference and working memory in rodents. This task relies on the rodent’s ability to orientate itself in the maze using extra-maze visual cues. This test can be used to investigate whether a rodent’s cognition is improved or impaired under a variety of experimental ...

Assessment of Mechanical Allodynia in Rats Using the Electronic Von Frey Test

Authors: Jeremy Ferrier*
Jeremy FerrierAffiliation 1: Centre national de la recherche scientifique (CNRS), Paris, France
Affiliation 2: Brain Plasticity Unit, ESPCI-ParisTech, PSL Research University, Paris, France
Bio-protocol author page: a3513
, Fabien Marchand*
Fabien MarchandAffiliation 1: Institut national de la santé et de la recherche médicale (INSERM), Clermont-Ferrand, France
Affiliation 2: Clermont Université, Université d’Auvergne, Clermont-Ferrand, France
Bio-protocol author page: a3514
and David Balayssac
David BalayssacAffiliation 1: Institut national de la santé et de la recherche médicale (INSERM), Clermont-Ferrand, France
Affiliation 2: Clermont Université, Université d’Auvergne, Clermont-Ferrand, France
Affiliation 3: Centre Hospitalier Universitaire (CHU), Clermont-Ferrand, France
For correspondence: dbalayssac@chu-clermontferrand.fr
Bio-protocol author page: a3515
(*contributed equally to this work), date: 9/20/2016, 2084 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1933.

[Abstract] Chronic pain is one of the most debilitating conditions, affecting one out of five people worldwide. Preclinical models in rodents represent a valuable tool for the study of pathophysiological mechanisms and the discovery of new analgesic drugs. However, pain evaluation in rodents is rather challenging. ...

Locomotion Activity Measurement in an Open Field for Mice

Authors: Levente Gellért
Levente GellértAffiliation 1: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Affiliation 2: MTA-SZTE Neuroscience Research Group, Szeged, Hungary
For correspondence: gellert.levente1@gmail.com
Bio-protocol author page: a3274
and Dániel Varga
Dániel VargaAffiliation: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Bio-protocol author page: a3275
, date: 7/5/2016, 1990 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1857.

[Abstract] The Open Field (OF) paradigm is one of the most known primary behavioral tests to evaluate locomotion and exploration. Altered OF behaviour is relatively simple to observe, but understanding and explaining the reasons for the observed changes is a complex task. Generally, there are two factors, which ...

Running Reward Conditioned Place Preference Task

Authors: Maria Fernanda A. Fernandes
Maria Fernanda A. FernandesAffiliation 1: Centre de Recherche du CHUM and Montreal Diabetes Research Center, Universite de Montreal, Montreal, QC, Canada
Affiliation 2: Department of Physiology, CHUM Research Center, Universite de Montreal, Montreal, QC, Canada
For correspondence: mariafer@uoguelph.ca
Bio-protocol author page: a3149
and Stephanie Fulton
Stephanie FultonAffiliation 1: Centre de Recherche du CHUM and Montreal Diabetes Research Center, Universite de Montreal, Montreal, QC, Canada
Affiliation 2: Department of Nutrition, Universite de Montreal, Montreal, QC, Canada
Affiliation 3: Center for Studies in Behavioral Neurobiology, Concordia University, Montreal, Montreal, QC, Canada
Bio-protocol author page: a3150
, date: 5/20/2016, 1938 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1816.

[Abstract] The conditioned place preference (CPP) test is a standard pre-clinical behavioral tool used to study the motivational effects of drugs and non-drug treatments in experimental animals. The basic characteristic of this task involves the association of a particular environment and contextual cues with ...

Assessment of Mechanical Allodynia in Rats Using the Electronic Von Frey Test

Authors: Jeremy Ferrier*
Jeremy FerrierAffiliation 1: Centre national de la recherche scientifique (CNRS), Paris, France
Affiliation 2: Brain Plasticity Unit, ESPCI-ParisTech, PSL Research University, Paris, France
Bio-protocol author page: a3513
, Fabien Marchand*
Fabien MarchandAffiliation 1: Institut national de la santé et de la recherche médicale (INSERM), Clermont-Ferrand, France
Affiliation 2: Clermont Université, Université d’Auvergne, Clermont-Ferrand, France
Bio-protocol author page: a3514
and David Balayssac
David BalayssacAffiliation 1: Institut national de la santé et de la recherche médicale (INSERM), Clermont-Ferrand, France
Affiliation 2: Clermont Université, Université d’Auvergne, Clermont-Ferrand, France
Affiliation 3: Centre Hospitalier Universitaire (CHU), Clermont-Ferrand, France
For correspondence: dbalayssac@chu-clermontferrand.fr
Bio-protocol author page: a3515
(*contributed equally to this work), date: 9/20/2016, 2084 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1933.

Locomotion Activity Measurement in an Open Field for Mice

Authors: Levente Gellért
Levente GellértAffiliation 1: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Affiliation 2: MTA-SZTE Neuroscience Research Group, Szeged, Hungary
For correspondence: gellert.levente1@gmail.com
Bio-protocol author page: a3274
and Dániel Varga
Dániel VargaAffiliation: Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Bio-protocol author page: a3275
, date: 7/5/2016, 1990 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1857.

Running Reward Conditioned Place Preference Task

Authors: Maria Fernanda A. Fernandes
Maria Fernanda A. FernandesAffiliation 1: Centre de Recherche du CHUM and Montreal Diabetes Research Center, Universite de Montreal, Montreal, QC, Canada
Affiliation 2: Department of Physiology, CHUM Research Center, Universite de Montreal, Montreal, QC, Canada
For correspondence: mariafer@uoguelph.ca
Bio-protocol author page: a3149
and Stephanie Fulton
Stephanie FultonAffiliation 1: Centre de Recherche du CHUM and Montreal Diabetes Research Center, Universite de Montreal, Montreal, QC, Canada
Affiliation 2: Department of Nutrition, Universite de Montreal, Montreal, QC, Canada
Affiliation 3: Center for Studies in Behavioral Neurobiology, Concordia University, Montreal, Montreal, QC, Canada
Bio-protocol author page: a3150
, date: 5/20/2016, 1938 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1816.

The Object Context-place-location Paradigm for Testing Spatial Memory in Mice

Authors: Edith Lesburguères
Edith LesburguèresAffiliation: Center for Neural Science, New York University, New York, USA
Bio-protocol author page: a4246
, Panayiotis Tsokas
Panayiotis TsokasAffiliation 1: Department of Physiology and Pharmacology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Affiliation 2: Department of Anesthesiology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Bio-protocol author page: a4243
, Todd Charlton Sacktor
Todd Charlton SacktorAffiliation 1: Department of Physiology and Pharmacology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Affiliation 2: Department of Neurology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
Bio-protocol author page: a4244
and André Antonio Fenton
André Antonio FentonAffiliation 1: Center for Neural Science, New York University, New York, USA
Affiliation 2: Department of Physiology and Pharmacology, the Robert F. Furchgott Center for Neural and Behavioral Science, Downstate Medical Center, State University of New York, New York, USA
For correspondence: afenton@nyu.edu
Bio-protocol author page: a4245
, date: 4/20/2017, 525 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2231.

Olfactory Cued Learning Paradigm

Authors: Gary Liu
Gary LiuAffiliation 1: Program in Developmental Biology, Baylor College of Medicine, Houston, USA
Affiliation 2: Medical Scientist Training Program, Baylor College of Medicine, Houston, USA
For correspondence: garyliu87@gmail.com
Bio-protocol author page: a4435
, Cynthia K. McClard*
Cynthia K. McClardAffiliation 1: Medical Scientist Training Program, Baylor College of Medicine, Houston, USA
Affiliation 2: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4436
, Burak Tepe*
Burak TepeAffiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4437
, Jessica Swanson
Jessica SwansonAffiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4438
, Brandon Pekarek
Brandon PekarekAffiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Bio-protocol author page: a4439
, Sugi Panneerselvam
Sugi PanneerselvamAffiliation: Rice University, Houston, USA
Bio-protocol author page: a4440
and Benjamin R. Arenkiel
Benjamin R. ArenkielAffiliation 1: Program in Developmental Biology, Baylor College of Medicine, Houston, USA
Affiliation 2: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, USA
Affiliation 3: Department of Neuroscience, Baylor College of Medicine, Houston, USA
Affiliation 4: Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, USA
Bio-protocol author page: a4441
(*contributed equally to this work), date: 5/5/2017, 342 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2251.

Stereotaxic Surgery for Suprachiasmatic Nucleus Lesions in Mice

Authors: Kimiko Shimizu
Kimiko ShimizuAffiliation: Department of Biological Sciences, School of Science, the University of Tokyo, Tokyo, 113-0033 Japan
For correspondence: shimizuk@bs.s.u-tokyo.ac.jp
Bio-protocol author page: a4698
and Yoshitaka Fukada
Yoshitaka FukadaAffiliation: Department of Biological Sciences, School of Science, the University of Tokyo, Tokyo, 113-0033 Japan
Bio-protocol author page: a4699
, date: 6/20/2017, 198 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2346.

Mammalia

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