Mammalia

Measurement of Dipeptidylpeptidase Activity in vitro and in vivo

Authors: Rosa Barreira da Silva
Rosa Barreira da SilvaAffiliation: Cancer Immunology, Genentech, South San Francisco, CA, USA
Bio-protocol author page: a4161
, Molly A. Ingersoll
Molly A. IngersollAffiliation 1: Laboratory of Dendritic Cell Immunobiology, Institut Pasteur, Paris, France
Affiliation 2: INSERM U1223, Paris, France
Bio-protocol author page: a4162
and Matthew L. Albert
Matthew L. AlbertAffiliation 1: Cancer Immunology, Genentech, South San Francisco, CA, USA
Affiliation 2: Laboratory of Dendritic Cell Immunobiology, Institut Pasteur, Paris, France
Affiliation 3: INSERM U1223, Paris, France
For correspondence: albertm7@gene.com
Bio-protocol author page: a4163
, date: 3/20/2017, 561 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.2184.

[Abstract] Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is constitutively expressed in the extracellular space. DPP4 is found ...

Measurement of Transferrin- and Non-transferrin-bound Iron Uptake by Mouse Tissues

Authors: Supak Jenkitkasemwong
Supak JenkitkasemwongAffiliation: Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida, USA
Bio-protocol author page: a3485
, Chia-Yu Wang
Chia-Yu WangAffiliation: Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida, USA
Bio-protocol author page: a3486
and Mitchell D. Knutson
Mitchell D. KnutsonAffiliation: Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida, USA
For correspondence: mknutson@ufl.edu
Bio-protocol author page: a3487
, date: 9/5/2016, 1238 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1922.

[Abstract] Iron in blood plasma is bound to its transport protein transferrin, which delivers iron to most tissues. In iron overload and certain pathological conditions, the carrying capacity of transferrin can become exceeded, giving rise to non-transferrin-bound iron, which is taken up preferentially by the ...

Measurement of 2-methylthio Modifications in Mitochondrial Transfer RNAs by Reverse-transcription Quantitative PCR

Authors: Fan-Yan Wei
Fan-Yan WeiAffiliation: Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan
For correspondence: fywei@kumamoto-u.ac.jp
Bio-protocol author page: a2824
and Kazuhito Tomizawa
Kazuhito TomizawaAffiliation: Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan
Bio-protocol author page: a2825
, date: 1/5/2016, 1498 views, 0 Q&A
DOI: https://doi.org/10.21769/BioProtoc.1695.

[Abstract] 2-Methylthio-N⁶-isopentenyladenosine (ms²i⁶A) is an evolutionally conserved posttranscriptional modification found at position 37 of four mammalian mitochondrial tRNAs, mt-tRNA^Ser(UCN), mt-tRNA^Trp, mt-tRNA^Phe and mt-tRNA^Tyr. The ms² modification in ms²i⁶A strengthens codon-anticodon interaction and ...

	In vitro Culture of Human PBMCs
	Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
	Analysis of Protein Stability by the Cycloheximide Chase Assay
	Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
	Dimethylmethylene Blue Assay (DMMB)
	Isolation of Human PBMCs
	BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
	Analysis of Intestinal Permeability in Mice
	Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
	Subcellular Fractionation of Cultured Human Cell Lines